?url_ver=Z39.88-2004&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Adc&rft.title=Super-Beacons%3A+open-source+probes+with+spontaneous+tuneable+blinking+compatible+with+live-cell+super-resolution+microscopy&rft.creator=Pereira%2C+P&rft.creator=Gustafsson%2C+N&rft.creator=Marsh%2C+M&rft.creator=Mhlanga%2C+M&rft.creator=Henriques%2C+R&rft.description=Localization+based+super-resolution+microscopy+relies+on+the+detection+of+individual+molecules+cycling+between+fluorescent+and+non-fluorescent+states.+These+transitions+are+commonly+regulated+by+high-intensity+illumination%2C+imposing+constrains+to+imaging+hardware+and+producing+sample+photodamage.+Here%2C+we+propose+single-molecule+self-quenching+as+a+mechanism+to+generate+spontaneous+photoswitching+independent+of+illumination.+To+demonstrate+this+principle%2C+we+developed+a+new+class+of+DNA-based+open-source+Super-Resolution+probes+named+Super-Beacons%2C+with+photoswitching+kinetics+that+can+be+tuned+structurally%2C+thermally+and+chemically.+The+potential+of+these+probes+for+live-cell+friendly+Super-Resolution+Microscopy+without+high-illumination+or+toxic+imaging+buffers+is+revealed+by+imaging+Interferon+Inducible+Transmembrane+proteins+(IFITMs)+at+sub-100nm+resolutions.&rft.publisher=BioRxiv&rft.date=2020-02-04&rft.type=Working+%2F+discussion+paper&rft.language=eng&rft.source=++++BioRxiv%3A+Cold+Spring+Harbor%2C+NY%2C+USA.+(2020)+++++&rft.format=text&rft.identifier=https%3A%2F%2Fdiscovery.ucl.ac.uk%2Fid%2Feprint%2F10090448%2F1%2F2020.01.20.912311v1.full.pdf&rft.identifier=https%3A%2F%2Fdiscovery.ucl.ac.uk%2Fid%2Feprint%2F10090448%2F&rft.rights=open