eprintid: 10085286
rev_number: 24
eprint_status: archive
userid: 608
dir: disk0/10/08/52/86
datestamp: 2019-11-08 12:37:54
lastmod: 2021-11-16 00:47:39
status_changed: 2019-11-08 12:37:54
type: article
metadata_visibility: show
creators_name: Eldrid, C
creators_name: O'Connor, E
creators_name: Thalassinos, K
title: Concentration‐dependent coulombic effects in travelling wave ion mobility spectrometry collision cross section calibration
ispublished: pub
divisions: UCL
divisions: B02
divisions: C08
divisions: D09
divisions: G03
note: This version is the author accepted manuscript. For information on re-use, please refer to the publisher’s terms and conditions.
abstract: RATIONALE: Travelling wave ion mobility spectrometry (TWIMS) is increasingly being used as a method for calculating the collision cross section (CCS) of protein ions. To calculate the CCS of unknown ions, however, the TWIMS device needs to be calibrated using calibrant proteins of known CCS values. The effect of calibrant protein concentration of the accuracy of the resulting calibration curve has not been explicitly studied so far. We hypothesised that at high protein concentrations the ion density within the TWIMS device will be such that ions will experience space charge effects resulting in deviations, as well as broadening, of ion arrival time distributions (ATDs). Calibration curves using these altered ATDs would therefore result in incorrect CCS values being calculated for the protein ions of interest. // METHODS: Three protein CCS calibrants, avidin, bovine serum albumin and β-lactgobulin, were prepared at different concentrations and used to calculate the CCS of a non-calibrant protein. Data were collected on a Synapt G1 ion mobility-mass spectrometer with a nano-electrospray ionisation (nESI) source using capillaries prepared in house. // RESULTS: Increasing the concentration of CCS calibrants caused ATD broadening and shifted the ATD peak tops, leading to a significant increase in calculated CCS values. // CONCLUSION: The concentration of protein calibrants can directly affect the quality of the CCS calibration in TWIMS experiments.
date: 2020-09
date_type: published
official_url: https://doi.org/10.1002/rcm.8613
oa_status: green
full_text_type: other
language: eng
primo: open
primo_central: open_green
verified: verified_manual
elements_id: 1714721
doi: 10.1002/rcm.8613
lyricists_name: Eldrid, Charles
lyricists_name: Thalassinos, Konstantinos
lyricists_id: CFSEL09
lyricists_id: KTHAL22
actors_name: Flynn, Bernadette
actors_id: BFFLY94
actors_role: owner
full_text_status: public
publication: Rapid Communications in Mass Spectrometry
volume: 34
number: S4
article_number: e8613
event_location: England
issn: 1097-0231
citation:        Eldrid, C;    O'Connor, E;    Thalassinos, K;      (2020)    Concentration‐dependent coulombic effects in travelling wave ion mobility spectrometry collision cross section calibration.                   Rapid Communications in Mass Spectrometry , 34  (S4)    , Article e8613.  10.1002/rcm.8613 <https://doi.org/10.1002/rcm.8613>.       Green open access   
 
document_url: https://discovery.ucl.ac.uk/id/eprint/10085286/1/Eldrid_et_al-2019-Rapid_Communications_in_Mass_Spectrometry.pdf