eprintid: 10084671
rev_number: 20
eprint_status: archive
userid: 608
dir: disk0/10/08/46/71
datestamp: 2019-11-01 13:53:00
lastmod: 2021-09-27 22:47:17
status_changed: 2019-11-01 13:53:00
type: article
metadata_visibility: show
creators_name: Dragojević, T
creators_name: Vidal Rosas, EE
creators_name: Hollmann, JL
creators_name: Culver, JP
creators_name: Justicia, C
creators_name: Durduran, T
title: High-density speckle contrast optical tomography of cerebral blood flow response to functional stimuli in the rodent brain
ispublished: pub
divisions: UCL
divisions: B04
divisions: C05
divisions: F42
keywords: blood or tissue constituent monitoring, functional monitoring and imaging, medical and biological imaging, speckle imaging
note: Copyright © The Authors. Published by SPIE under a Creative Commons Attribution 4.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.
abstract: Noninvasive, three-dimensional, and longitudinal imaging of cerebral blood flow (CBF) in small animal models and ultimately in humans has implications for fundamental research and clinical applications. It enables the study of phenomena such as brain development and learning and the effects of pathologies, with a clear vision for translation to humans. Speckle contrast optical tomography (SCOT) is an emerging optical method that aims to achieve this goal by directly measuring three-dimensional blood flow maps in deep tissue with a relatively inexpensive and simple system. High-density SCOT is developed to follow CBF changes in response to somatosensory cortex stimulation. Measurements are carried out through the intact skull on the rat brain. SCOT is able to follow individual trials in each brain hemisphere, where signal averaging resulted in comparable, cortical images to those of functional magnetic resonance images in spatial extent, location, and depth. Sham stimuli are utilized to demonstrate that the observed response is indeed due to local changes in the brain induced by forepaw stimulation. In developing and demonstrating the method, algorithms and analysis methods are developed. The results pave the way for longitudinal, nondestructive imaging in preclinical rodent models that can readily be translated to the human brain.
date: 2019-10
date_type: published
official_url: https://doi.org/10.1117/1.NPh.6.4.045001
oa_status: green
full_text_type: pub
pmcid: PMC6782685
language: eng
primo: open
primo_central: open_green
verified: verified_manual
elements_id: 1712560
doi: 10.1117/1.NPh.6.4.045001
pii: 19070R
lyricists_name: Vidal Rosas, Ernesto
lyricists_id: EEVID97
actors_name: Allington-Smith, Dominic
actors_id: DAALL44
actors_role: owner
full_text_status: public
publication: Neurophotonics
volume: 6
number: 4
article_number: 045001
event_location: United States
citation:        Dragojević, T;    Vidal Rosas, EE;    Hollmann, JL;    Culver, JP;    Justicia, C;    Durduran, T;      (2019)    High-density speckle contrast optical tomography of cerebral blood flow response to functional stimuli in the rodent brain.                   Neurophotonics , 6  (4)    , Article 045001.  10.1117/1.NPh.6.4.045001 <https://doi.org/10.1117/1.NPh.6.4.045001>.       Green open access   
 
document_url: https://discovery.ucl.ac.uk/id/eprint/10084671/7/Vidal%20Rosas_045001_1.pdf