TY  - JOUR
N1  - This article is distributed under the terms of an Attribution?Noncommercial?Share Alike?No Mirror Sites license for the first six months after the
publication date. After six months it is available under a Creative Commons License (Attribution?Noncommercial?Share Alike 4.0
International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/).
SP  - 4171
TI  - Two S. pombe septation phases differ in ingression rate, septum structure, and response to F-actin loss
Y1  - 2019/10/09/
UR  - https://doi.org/10.1083/jcb.201808163
VL  - 218
A1  - Ramos, M
A1  - Cortés, JCG
A1  - Sato, M
A1  - Rincón, SA
A1  - Moreno, MB
A1  - Clemente-Ramos, JÁ
A1  - Osumi, M
A1  - Pérez, P
A1  - Ribas, JC
EP  - 4194
AV  - public
N2  - In fission yeast, cytokinesis requires a contractile actomyosin ring (CR) coupled to membrane and septum ingression. Septation proceeds in two phases. In anaphase B, the septum ingresses slowly. During telophase, the ingression rate increases, and the CR becomes dispensable. Here, we explore the relationship between the CR and septation by analyzing septum ultrastructure, ingression, and septation proteins in cells lacking F-actin. We show that the two phases of septation correlate with septum maturation and the response of cells to F-actin removal. During the first phase, the septum is immature and, following F-actin removal, rapidly loses the Bgs1 glucan synthase from the membrane edge and fails to ingress. During the second phase, the rapidly ingressing mature septum can maintain a Bgs1 ring and septum ingression without F-actin, but ingression becomes Cdc42 and exocyst dependent. Our results provide new insights into fungal cytokinesis and reveal the dual function of CR as an essential landmark for the concentration of Bgs1 and a contractile structure that maintains septum shape and synthesis.
JF  - Journal of Cell Biology
ID  - discovery10083878
IS  - 12
ER  -