TY - JOUR SP - 1141 SN - 1097-0134 AV - public JF - Proteins IS - 12 KW - CASP KW - Protein structure prediction KW - deep learning KW - machine learning N1 - Copyright © 2019 The Authors. Proteins: Structure, Function, and Bioinformatics published by Wiley Periodicals, Inc. This is an open access article under the terms of the Creative Commons Attribution?NonCommercial?NoDerivs License (http://creativecommons.org/licenses/by-nc-nd/4.0/), which permits use and distribution in any medium, provided the original work is properly cited, the use is non?commercial and no modifications or adaptations are made. Y1 - 2019/12// TI - Protein structure prediction using multiple deep neural networks in the 13th Critical Assessment of Protein Structure Prediction (CASP13) N2 - We describe AlphaFold, the protein structure prediction system that was entered by the group A7D in CASP13 Submissions were made by three free-modelling methods which combine the predictions of three neural networks. All three systems were guided by predictions of distances between pairs of residues produced by a neural network. Two systems assembled fragments produced by a generative neural network, one using scores from a network trained to regress GDT_TS. The third system shows that simple gradient descent on a properly constructed potential is able to perform on-par with more expensive traditional search techniques and without requiring domain segmentation. In the CASP13 free-modelling assessors' ranking by summed z-scores, this system scored highest with 68.3 vs 48.2 for the next closest group. (An average GDT_TS of 61.4.) The system produced high-accuracy structures (with GDT_TS scores of 70 or higher) for 11 out of 43 free-modelling domains. Despite not explicitly using template information, the results in the template category were comparable to the best performing template-based methods. EP - 1148 A1 - Senior, AW A1 - Evans, R A1 - Jumper, J A1 - Kirkpatrick, J A1 - Sifre, L A1 - Green, T A1 - Qin, C A1 - ?ídek, A A1 - Nelson, AWR A1 - Bridgland, A A1 - Penedones, H A1 - Petersen, S A1 - Simonyan, K A1 - Crossan, S A1 - Kohli, P A1 - Jones, DT A1 - Silver, D A1 - Kavukcuoglu, K A1 - Hassabis, D UR - https://doi.org/10.1002/prot.25834 VL - 87 ID - discovery10083488 ER -