eprintid: 10083415 rev_number: 21 eprint_status: archive userid: 608 dir: disk0/10/08/34/15 datestamp: 2019-10-16 16:37:43 lastmod: 2021-09-26 23:13:22 status_changed: 2019-10-16 16:37:43 type: article metadata_visibility: show creators_name: Bayram, C creators_name: Jiang, X creators_name: Gultekinoglu, M creators_name: Ozturk, S creators_name: Ulubayram, K creators_name: Edirisinghe, M title: Biofabrication of Gelatin Tissue Scaffolds with Uniform Pore Size via Microbubble Assembly ispublished: pub divisions: UCL divisions: B04 divisions: C05 divisions: F45 keywords: biofabrication, gelatin, microbubbles, tissue scaffold note: This version is the author accepted manuscript. For information on re-use, please refer to the publisher’s terms and conditions. abstract: The control of pore size and uniform porosity remains as an important challenge in gelatin scaffolds. The precise control in building blocks of tissue scaffolds without any additional porogen is possible with costly equipment and techniques, though some pre‐requirements for polymeric material, such as photo‐polymerizability or sintering ability, may be needed prior to construction. Herein, a method for the fabrication of gelatin scaffolds with homogenous porosity using simple T‐junction microfluidics is described. The size of the microbubbles is precisely controlled with 5% deviation from the average. Porous gelatin scaffolds are obtained by building‐up the monodispersed microbubbles in dilute cross‐linker solutions. The effect of cross‐linker density on pore diameter is also investigated. After cross‐linking, pore size of the resultant five scaffold groups are precisely controlled as 135 ± 11, 193 ± 11, 216 ± 9, 231 ± 5, and 250 ± 12 µm. Porosity ratios above 65% are achieved in every sample group. According to the cell culture experiments, structures support high cell adhesion, viability, and migration through the porous network via interconnectivity. This study offers a practical and economical approach for the preparation of porous gelatin scaffolds with homogenous porosity which can be utilized in diverse tissue engineering applications. date: 2019-11 date_type: published publisher: WILEY-V C H VERLAG GMBH official_url: https://doi.org/10.1002/mame.201900394 oa_status: green full_text_type: other language: eng primo: open primo_central: open_green verified: verified_manual elements_id: 1686506 doi: 10.1002/mame.201900394 lyricists_name: Edirisinghe, Mohan lyricists_id: MJEDE48 actors_name: Edirisinghe, Mohan actors_id: MJEDE48 actors_role: owner full_text_status: public publication: Macromolecular Materials and Engineering volume: 304 number: 11 article_number: 1900394 pages: 9 issn: 1439-2054 citation: Bayram, C; Jiang, X; Gultekinoglu, M; Ozturk, S; Ulubayram, K; Edirisinghe, M; (2019) Biofabrication of Gelatin Tissue Scaffolds with Uniform Pore Size via Microbubble Assembly. Macromolecular Materials and Engineering , 304 (11) , Article 1900394. 10.1002/mame.201900394 <https://doi.org/10.1002/mame.201900394>. Green open access document_url: https://discovery.ucl.ac.uk/id/eprint/10083415/1/mame.201900394_R1.pdf