TY  - GEN
A1  - Sudakou, A
A1  - Lange, F
A1  - Isler, H
A1  - Gerega, A
A1  - Ostojic, D
A1  - Sawosz, P
A1  - Tachtsidis, I
A1  - Wolf, M
A1  - Liebert, A
UR  - https://doi.org/10.1117/12.2527215
N2  - Absolute concentrations of oxyhaemoglobin and deoxyhaemoglobin obtained using multi-wavelength measurements
of time-resolved diffuse reflectance signals are presented. The aim was to test how accurately the concentration of
oxy- and deoxyhemoglobin can be measured simultaneously at multiple wavelengths. The optical signals were
collected using the system constructed by the author?s group
a
, which records the distribution in time of flight of
photons (DTOFs) simultaneously at 16 spectral channels ranging from 650 to 850 nm. The measurements were
carried out on liquid phantoms containing intralipid solution, human blood and yeast in varying amounts. The
oxygen saturation of blood covered a continuous range from 100 % to 0 % during 6 deoxygenation cycles. The
estimated values of total haemoglobin (20.9, 35.7, 57.3, 45.7 ?M) are close to the reference values obtained using a
blood gas analyzer (21.3, 37.0, 57.3, 48.0 ?M) and the estimated values of absolute concentrations of oxy-, deoxyand total haemoglobin are similar to the values obtained using a commercial frequency-domain NIRS system
(OxiplexTS
TM). The phantom measurements have shown the capability to measure the absolute concentrations of
chromophores in a studied media using multi-wavelength, time-resolved NIRS technique. The excess number of
spectral channels can potentially be used to resolve changes in oxidation state of cytochrome-c-oxidase enzyme.
AV  - public
ID  - discovery10081768
KW  - diffuse optics
KW  -  time-resolved NIRS
KW  -  blood phantom
KW  -  oxygen saturation
CY  - Munich, Germany
PB  - SPIE
N1  - This version is the version of record. For information on re-use, please refer to the publisher?s terms and conditions.
TI  - Multi-wavelength time-resolved NIRS measurements for estimation of absolute concentration of chromophores: blood phantom study
Y1  - 2019/07/11/
ER  -