eprintid: 10076717
rev_number: 20
eprint_status: archive
userid: 608
dir: disk0/10/07/67/17
datestamp: 2019-06-26 12:09:07
lastmod: 2021-09-26 22:29:04
status_changed: 2019-06-26 12:09:07
type: article
metadata_visibility: show
creators_name: Pritchard, RH
creators_name: Zhukov, AA
creators_name: Fullerton, JN
creators_name: Want, AJ
creators_name: Hussain, F
creators_name: la Cour, MF
creators_name: Bashtanov, ME
creators_name: Gold, RD
creators_name: Hailes, A
creators_name: Banham-Hall, E
creators_name: Rogers, SS
title: Cell sorting actuated by a microfluidic inertial vortex
ispublished: inpress
divisions: UCL
divisions: B02
divisions: C10
divisions: D17
note: © The Royal Society of Chemistry 2019. This article is licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported Licence (http://creativecommons.org/licenses/by-nc/3.0/).
abstract: The sorting of specific cell populations is an established tool in biological research, with new applications demanding greater cell throughput, sterility and elimination of cross-contamination. Here we report ‘vortex-actuated cell sorting’ (VACS), a new technique that deflects cells individually, via the generation of a transient microfluidic vortex by a thermal vapour bubble: a novel mechanism, which is able to sort cells based on fluorescently-labelled molecular markers. Using in silico simulation and experiments on beads, an immortal cell line and human peripheral blood mononuclear cells (PBMCs), we demonstrate high-purity and high-recovery sorting with input rates up to 10^{4} cells per s and switching speeds comparable to existing techniques (>40 kHz). A tiny footprint (1 × 0.25 mm) affords miniaturization and the potential to achieve multiplexing: a crucial step in increasing processing rate. Simple construction using biocompatible materials potentially minimizes cost of fabrication and permits single-use sterile cartridges. We believe VACS potentially enables parallel sorting at throughputs relevant to cell therapy, liquid biopsy and phenotypic screening.
date: 2019-06-18
date_type: published
official_url: http://dx.doi.org/10.1039/c9lc00120d
oa_status: green
full_text_type: pub
language: eng
primo: open
primo_central: open_green
article_type_text: Journal Article
verified: verified_manual
elements_id: 1666663
doi: 10.1039/c9lc00120d
language_elements: English
lyricists_name: Fullerton, James
lyricists_id: JNFUL59
actors_name: Flynn, Bernadette
actors_id: BFFLY94
actors_role: owner
full_text_status: public
publication: Lab on a Chip
event_location: England
issn: 1473-0189
citation:        Pritchard, RH;    Zhukov, AA;    Fullerton, JN;    Want, AJ;    Hussain, F;    la Cour, MF;    Bashtanov, ME;                 ... Rogers, SS; + view all <#>        Pritchard, RH;  Zhukov, AA;  Fullerton, JN;  Want, AJ;  Hussain, F;  la Cour, MF;  Bashtanov, ME;  Gold, RD;  Hailes, A;  Banham-Hall, E;  Rogers, SS;   - view fewer <#>    (2019)    Cell sorting actuated by a microfluidic inertial vortex.                   Lab on a Chip        10.1039/c9lc00120d <https://doi.org/10.1039/c9lc00120d>.    (In press).    Green open access   
 
document_url: https://discovery.ucl.ac.uk/id/eprint/10076717/7/Fullerton_Cell%20sorting%20actuated%20by%20a%20microfluidic%20inertial%20vortex_AOP.pdf