TY  - INPR
JF  - Lab on a Chip
A1  - Pritchard, RH
A1  - Zhukov, AA
A1  - Fullerton, JN
A1  - Want, AJ
A1  - Hussain, F
A1  - la Cour, MF
A1  - Bashtanov, ME
A1  - Gold, RD
A1  - Hailes, A
A1  - Banham-Hall, E
A1  - Rogers, SS
N2  - The sorting of specific cell populations is an established tool in biological research, with new applications demanding greater cell throughput, sterility and elimination of cross-contamination. Here we report ?vortex-actuated cell sorting? (VACS), a new technique that deflects cells individually, via the generation of a transient microfluidic vortex by a thermal vapour bubble: a novel mechanism, which is able to sort cells based on fluorescently-labelled molecular markers. Using in silico simulation and experiments on beads, an immortal cell line and human peripheral blood mononuclear cells (PBMCs), we demonstrate high-purity and high-recovery sorting with input rates up to 10^{4} cells per s and switching speeds comparable to existing techniques (>40 kHz). A tiny footprint (1 × 0.25 mm) affords miniaturization and the potential to achieve multiplexing: a crucial step in increasing processing rate. Simple construction using biocompatible materials potentially minimizes cost of fabrication and permits single-use sterile cartridges. We believe VACS potentially enables parallel sorting at throughputs relevant to cell therapy, liquid biopsy and phenotypic screening.
ID  - discovery10076717
UR  - http://dx.doi.org/10.1039/c9lc00120d
SN  - 1473-0189
N1  - © The Royal Society of Chemistry 2019. This article is licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported Licence (http://creativecommons.org/licenses/by-nc/3.0/).
TI  - Cell sorting actuated by a microfluidic inertial vortex
Y1  - 2019/06/18/
AV  - public
ER  -