TY - JOUR Y1 - 2019/07/22/ TI - Design and use of de novo cascades for new benzylisoquinoline alkaloid biosynthesis VL - 58 KW - Biocatalysis KW - benzylisoquinoline alkaloids KW - norcoclaurine synthase KW - enzyme cascades A1 - Hailes, H A1 - Ward, J A1 - Wang, Y A1 - Tappertzhofen, N A1 - Mendez-Sanchez, D A1 - Bawn, M A1 - Lyu, B EP - 10125 IS - 30 UR - https://doi.org/10.1002/anie.201902761 ID - discovery10074317 N1 - This version is the author accepted manuscript. For information on re-use, please refer to the publisher?s terms and conditions. JF - Angewandte Chemie PB - Wiley-VCH Verlag N2 - The benzylisoquinoline alkaloids (BIAs) are an important group of higher plant secondary metabolites, which have been reported to show significant pharmaceutical activities. The production of BIAs via synthetic biology approaches provides a higher yielding strategy compared to traditional synthetic methods and plant isolation methods. However, the reconstruction of BIAs pathways in microorganisms combining heterologous enzymes can also give access to BIAs via cascade reactions. Most importantly, non?natural BIAs can be generated through such artificial pathways. In the current study, we describe new tyrosinases and decarboxylases and combine these with a transaminase enzyme and norcoclaurine synthase for the efficient synthesis of several BIAs, including six non?natural alkaloids, in cascades from L?tyrosine and analogues. SN - 0044-8249 SP - 10120 AV - public ER -