eprintid: 10070866 rev_number: 17 eprint_status: archive userid: 608 dir: disk0/10/07/08/66 datestamp: 2019-05-02 12:02:47 lastmod: 2021-09-20 00:17:02 status_changed: 2019-05-02 12:02:47 type: article metadata_visibility: show creators_name: Laha, D creators_name: Johnen, P creators_name: Azevedo, C creators_name: Dynowski, M creators_name: Weiss, M creators_name: Capolicchio, S creators_name: Mao, H creators_name: Iven, T creators_name: Steenbergen, M creators_name: Freyer, M creators_name: Gaugler, P creators_name: De Campos, MKF creators_name: Zheng, N creators_name: Feussner, I creators_name: Jessen, HJ creators_name: Van Wees, SCM creators_name: Saiardi, A creators_name: Schaaf, G title: VIH2 Regulates the Synthesis of Inositol Pyrophosphate InsP₈ and Jasmonate-Dependent Defenses in Arabidopsis ispublished: pub divisions: UCL divisions: B02 divisions: C08 divisions: D77 note: This version is the version of record. For information on re-use, please refer to the publisher’s terms and conditions. abstract: Diphosphorylated inositol polyphosphates, also referred to as inositol pyrophosphates, are important signaling molecules that regulate critical cellular activities in many eukaryotic organisms, such as membrane trafficking, telomere maintenance, ribosome biogenesis, and apoptosis. In mammals and fungi, two distinct classes of inositol phosphate kinases mediate biosynthesis of inositol pyrophosphates: Kcs1/IP6K- and Vip1/PPIP5K-like proteins. Here, we report that PPIP5K homologs are widely distributed in plants and that Arabidopsis thaliana VIH1 and VIH2 are functional PPIP5K enzymes. We show a specific induction of inositol pyrophosphate InsP8 by jasmonate and demonstrate that steady state and jasmonate-induced pools of InsP8 in Arabidopsis seedlings depend on VIH2. We identify a role of VIH2 in regulating jasmonate perception and plant defenses against herbivorous insects and necrotrophic fungi. In silico docking experiments and radioligand binding-based reconstitution assays show high-affinity binding of inositol pyrophosphates to the F-box protein COI1-JAZ jasmonate coreceptor complex and suggest that coincidence detection of jasmonate and InsP8 by COI1-JAZ is a critical component in jasmonate-regulated defenses. date: 2015-04-01 date_type: published publisher: AMER SOC PLANT BIOLOGISTS official_url: https://doi.org/10.1105/tpc.114.135160 oa_status: green full_text_type: pub language: eng primo: open primo_central: open_green verified: verified_manual elements_id: 1029970 lyricists_name: Azevedo, Cristina lyricists_name: Laha, Debabrata lyricists_name: Saiardi, Adolfo lyricists_id: AZEVE40 lyricists_id: DLAHA15 lyricists_id: ASAIA50 actors_name: Laha, Debabrata actors_id: DLAHA15 actors_role: owner full_text_status: public publication: The Plant Cell volume: 27 number: 4 pagerange: 1082-1097 pages: 16 issn: 1532-298X citation: Laha, D; Johnen, P; Azevedo, C; Dynowski, M; Weiss, M; Capolicchio, S; Mao, H; ... Schaaf, G; + view all <#> Laha, D; Johnen, P; Azevedo, C; Dynowski, M; Weiss, M; Capolicchio, S; Mao, H; Iven, T; Steenbergen, M; Freyer, M; Gaugler, P; De Campos, MKF; Zheng, N; Feussner, I; Jessen, HJ; Van Wees, SCM; Saiardi, A; Schaaf, G; - view fewer <#> (2015) VIH2 Regulates the Synthesis of Inositol Pyrophosphate InsP₈ and Jasmonate-Dependent Defenses in Arabidopsis. The Plant Cell , 27 (4) pp. 1082-1097. Green open access document_url: https://discovery.ucl.ac.uk/id/eprint/10070866/1/Laha%20et%20al_The%20Plant%20Cell%202015.pdf