TY  - JOUR
TI  - Y-linked iDmrt1 paralogue (iDMY) in the Eastern spiny lobster, Sagmariasus verreauxi: The first invertebrate sex-linked Dmrt
A1  - Chandler, JC
A1  - Fitzgibbon, QP
A1  - Smith, G
A1  - Elizur, A
A1  - Ventura, T
Y1  - 2017/10/15/
UR  - https://doi.org/10.1016/j.ydbio.2017.08.031
N2  - Sex determination pathways are extensively diverse across species, with the master sex-determinants being the most variable element. Despite this, there is a family of DM-domain transcription factors (Dmrts), which hold a highly conserved function in sexual development. This work is the first to describe a heterogametic sex-linked Dmrt in an invertebrate species, the Eastern spiny lobster, Sagmariasus verreauxi. We have termed the Y-linked, truncated paralogue of the autosomal iDmrt1, Sv-iDMY. Considering the master sex-determining function of both DMY in medaka and DM-W in frog, we hypothesised a similar function of Sv-iDMY. By conducting temporal expression analyses during embryogenesis we have identified a putative male sex-determining period during which iDMY>iDmrt1. Employing a GAL4-transactivation assay we then demonstrate the dominant negative suppression of iDMY over its autosomal iDmrt1 paralogue, suggesting the mechanism with which iDMY determines sex. Comparative analyses of Sv-iDMY, DM-W and medaka DMY, highlight the C?-mediated features of oligomerisation and transactivation as central to the mechanism that each exerts. Indeed, these features may underpin the plasticity facilitating the convergent emergence of these three sporadic sex-linked master-Dmrts.
SP  - 337
IS  - 2
VL  - 430
PB  - ACADEMIC PRESS INC ELSEVIER SCIENCE
SN  - 1095-564X
JF  - Developmental Biology
N1  - This version is the author accepted manuscript. For information on re-use, please refer to the publisher?s terms and conditions.
EP  - 345
ID  - discovery10068273
AV  - public
KW  - Master sex-determinant
KW  -  Dmrt
KW  -  DMY
KW  -  Transactivation domain
KW  -  Crustacea
ER  -