eprintid: 10062801 rev_number: 23 eprint_status: archive userid: 608 dir: disk0/10/06/28/01 datestamp: 2018-12-03 13:24:24 lastmod: 2021-09-19 22:19:22 status_changed: 2018-12-03 13:24:24 type: article metadata_visibility: show creators_name: Teunissen, CE creators_name: Chiu, M-J creators_name: Yang, C-C creators_name: Yang, S-Y creators_name: Scheltens, P creators_name: Zetterberg, H creators_name: Blennow, K title: Plasma Amyloid-beta (A beta(42)) Correlates with Cerebrospinal Fluid A beta(42) in Alzheimer's Disease ispublished: pub divisions: UCL divisions: B02 divisions: C07 divisions: D07 divisions: F86 keywords: Amyloid-β , cerebrospinal fluid, immunomagnetic reduction, plasma note: This version is the author accepted manuscript. For information on re-use, please refer to the publisher’s terms and conditions. abstract: The 42 amino acid form of amyloid-β (Aβ42) plays a key role in the pathogenesis of Alzheimer’s disease (AD) and is a core biomarker for the diagnosis of AD. Numerous studies have shown that cerebrospinal fluid (CSF) Aβ42 concentrations are decreased in AD, when measured by enzyme-linked immunosorbent assay (ELISA) and other conventional immunoassays. While most studies report no change in plasma Aβ42, independent studies using the immunomagnetic reduction (IMR) technique report an increase in plasma Aβ42 levels in AD. To confirm the opposite changes of Aβ42 levels in CSF and plasma for AD, we assayed the levels of Aβ42 in plasma of subjects with known CSF Aβ42 levels. In total 43 controls and 63 AD patients were selected at two sites: the VU University Medical Center (n = 55) and Sahlgrenska University Hospital (n = 51). IMR and ELISA were applied to assay Aβ42 in plasma and CSF, respectively. We found a moderately negative correlation between plasma and CSF Aβ42 levels in AD patients (r = –0.352), and a weakly positive correlation in controls (r = 0.186). These findings further corroborate that there are opposite changes of Aβ42 levels in CSF and plasma in AD. The possible causes for the negative correlation are discussed by taken assay technologies, Aβ42 transport from brain to peripheral blood, and sample matrix into account. date: 2018 date_type: published publisher: IOS PRESS official_url: https://doi.org/10.3233/JAD-170784 oa_status: green full_text_type: other language: eng primo: open primo_central: open_green verified: verified_manual elements_id: 1547328 doi: 10.3233/JAD-170784 lyricists_name: Zetterberg, Henrik lyricists_id: HZETT94 actors_name: Jayawardana, Anusha actors_id: AJAYA51 actors_role: owner full_text_status: public publication: Journal of Alzheimer's Disease volume: 62 number: 4 pagerange: 1857-1863 pages: 7 issn: 1875-8908 citation: Teunissen, CE; Chiu, M-J; Yang, C-C; Yang, S-Y; Scheltens, P; Zetterberg, H; Blennow, K; (2018) Plasma Amyloid-beta (A beta(42)) Correlates with Cerebrospinal Fluid A beta(42) in Alzheimer's Disease. Journal of Alzheimer's Disease , 62 (4) pp. 1857-1863. 10.3233/JAD-170784 <https://doi.org/10.3233/JAD-170784>. Green open access document_url: https://discovery.ucl.ac.uk/id/eprint/10062801/1/Zetterberg_Teunissen.pdf