eprintid: 10060068
rev_number: 36
eprint_status: archive
userid: 608
dir: disk0/10/06/00/68
datestamp: 2018-10-30 13:13:39
lastmod: 2021-09-23 22:48:10
status_changed: 2018-10-30 13:13:39
type: article
metadata_visibility: show
creators_name: Janus, J
creators_name: Kanber, B
creators_name: Mahbuba, W
creators_name: Beynon, C
creators_name: Ramnarine, KV
creators_name: Lambert, DG
creators_name: Samani, NJ
creators_name: Stringer, EJ
creators_name: Kelly, ME
title: A Preclinical Ultrasound Method for the Assessment of Vascular Disease Progression in Murine Models
ispublished: pub
divisions: UCL
divisions: B04
divisions: C05
divisions: F42
keywords: Vessel wall-motion, animal models, diagnostic imaging, in vivo, high-frequency ultrasound
note: This version is the author accepted manuscript. For information on re-use, please refer to the publisher’s terms and conditions.
abstract: INTRODUCTION: The efficacy of preclinical ultrasound at providing a quantitative assessment of mouse models of vascular disease is relatively unknown. In this study, preclinical ultrasound was used in combination with a semi-automatic image processing method to track arterial distension alterations in mouse models of abdominal aortic aneurysm and atherosclerosis. METHODS: Longitudinal B-mode ultrasound images of the abdominal aorta were acquired using a preclinical ultrasound scanner. Arterial distension was assessed using a semi-automatic image processing algorithm to track vessel wall motion over the cardiac cycle. A standard, manual analysis method was applied for comparison. RESULTS: Mean arterial distension was significantly lower in abdominal aortic aneurysm mice between day 0 and day 7 post-onset of disease (p < 0.01) and between day 0 and day 14 (p < 0.001), while no difference was observed in sham control mice. Manual analysis detected a significant decrease (p < 0.05) between day 0 and day 14 only. Atherosclerotic mice showed alterations in arterial distension relating to genetic modification and diet. Arterial distension was significantly lower (p < 0.05) in Ldlr−/−(++/−−) mice fed high-fat western diet when compared with both wild type (++/++) mice and Ldlr−/−(++/−−) mice fed chow diet. The manual method did not detect a significant difference between these groups. CONCLUSIONS: Arterial distension can be used as an early marker for the detection of arterial disease in murine models. The semi-automatic analysis method provided increased sensitivity to differences between experimental groups when compared to the manual analysis method.
date: 2019
date_type: published
official_url: https://doi.org/10.1177/1742271X18793919
oa_status: green
full_text_type: other
language: eng
primo: open
primo_central: open_green
verified: verified_manual
elements_id: 1586216
doi: 10.1177/1742271X18793919
lyricists_name: Kanber, Baris
lyricists_id: BKANB73
actors_name: Jayawardana, Anusha
actors_id: AJAYA51
actors_role: owner
full_text_status: public
publication: Ultrasound
volume: 27
number: 2
pagerange: 85-93
issn: 1743-1344
citation:        Janus, J;    Kanber, B;    Mahbuba, W;    Beynon, C;    Ramnarine, KV;    Lambert, DG;    Samani, NJ;         ... Kelly, ME; + view all <#>        Janus, J;  Kanber, B;  Mahbuba, W;  Beynon, C;  Ramnarine, KV;  Lambert, DG;  Samani, NJ;  Stringer, EJ;  Kelly, ME;   - view fewer <#>    (2019)    A Preclinical Ultrasound Method for the Assessment of Vascular Disease Progression in Murine Models.                   Ultrasound , 27  (2)   pp. 85-93.    10.1177/1742271X18793919 <https://doi.org/10.1177/1742271X18793919>.       Green open access   
 
document_url: https://discovery.ucl.ac.uk/id/eprint/10060068/1/Kanber_PDFsam_merge.pdf