eprintid: 10056832
rev_number: 21
eprint_status: archive
userid: 608
dir: disk0/10/05/68/32
datestamp: 2018-09-24 09:04:13
lastmod: 2021-12-06 00:44:54
status_changed: 2018-09-24 09:04:13
type: article
metadata_visibility: show
creators_name: Urbančič, V
creators_name: Butler, R
creators_name: Richier, B
creators_name: Peter, M
creators_name: Mason, J
creators_name: Livesey, FJ
creators_name: Holt, CE
creators_name: Gallop, JL
title: Filopodyan: An open-source pipeline for the analysis of filopodia
ispublished: pub
divisions: UCL
divisions: B02
divisions: D13
divisions: G22
keywords: Animals, Cell Line, Drosophila melanogaster, Embryo, Nonmammalian, Humans, Image Processing, Computer-Assisted, Microscopy, Fluorescence, Pseudopodia, User-Computer Interface, Xenopus laevis
note: © 2017 Urbančič et al.
https://creativecommons.org/licenses/by/4.0/
This article is available under a Creative Commons License (Attribution 4.0 International, as described at https://creativecommons.org/licenses/by/4.0/).
abstract: Filopodia have important sensory and mechanical roles in motile cells. The recruitment of actin regulators, such as ENA/VASP proteins, to sites of protrusion underlies diverse molecular mechanisms of filopodia formation and extension. We developed Filopodyan (filopodia dynamics analysis) in Fiji and R to measure fluorescence in filopodia and at their tips and bases concurrently with their morphological and dynamic properties. Filopodyan supports high-throughput phenotype characterization as well as detailed interactive editing of filopodia reconstructions through an intuitive graphical user interface. Our highly customizable pipeline is widely applicable, capable of detecting filopodia in four different cell types in vitro and in vivo. We use Filopodyan to quantify the recruitment of ENA and VASP preceding filopodia formation in neuronal growth cones, and uncover a molecular heterogeneity whereby different filopodia display markedly different responses to changes in the accumulation of ENA and VASP fluorescence in their tips over time.
date: 2017-10-02
date_type: published
official_url: http://dx.doi.org/10.1083/jcb.201705113
oa_status: green
full_text_type: pub
pmcid: PMC5626553
language: eng
primo: open
primo_central: open_green
article_type_text: Journal Article
verified: verified_manual
elements_id: 1585445
doi: 10.1083/jcb.201705113
pii: jcb.201705113
lyricists_name: Livesey, Frederick
lyricists_id: FLIVE31
actors_name: Bracey, Alan
actors_id: ABBRA90
actors_role: owner
full_text_status: public
publication: Journal of Cell Biology
volume: 216
number: 10
pagerange: 3405-3422
event_location: United States
issn: 1540-8140
citation:        Urbančič, V;    Butler, R;    Richier, B;    Peter, M;    Mason, J;    Livesey, FJ;    Holt, CE;           Urbančič, V;  Butler, R;  Richier, B;  Peter, M;  Mason, J;  Livesey, FJ;  Holt, CE;  Gallop, JL;   - view fewer <#>    (2017)    Filopodyan: An open-source pipeline for the analysis of filopodia.                   Journal of Cell Biology , 216  (10)   pp. 3405-3422.    10.1083/jcb.201705113 <https://doi.org/10.1083/jcb.201705113>.       Green open access   
 
document_url: https://discovery.ucl.ac.uk/id/eprint/10056832/1/3405.full.pdf