TY  - JOUR
N1  - © 2017 Urban?i? et al.
https://creativecommons.org/licenses/by/4.0/
This article is available under a Creative Commons License (Attribution 4.0 International, as described at https://creativecommons.org/licenses/by/4.0/).
IS  - 10
SP  - 3405
VL  - 216
JF  - Journal of Cell Biology
A1  - Urban?i?, V
A1  - Butler, R
A1  - Richier, B
A1  - Peter, M
A1  - Mason, J
A1  - Livesey, FJ
A1  - Holt, CE
A1  - Gallop, JL
SN  - 1540-8140
UR  - http://dx.doi.org/10.1083/jcb.201705113
TI  - Filopodyan: An open-source pipeline for the analysis of filopodia
EP  - 3422
AV  - public
Y1  - 2017/10/02/
KW  - Animals
KW  -  Cell Line
KW  -  Drosophila melanogaster
KW  -  Embryo
KW  -  Nonmammalian
KW  -  Humans
KW  -  Image Processing
KW  -  Computer-Assisted
KW  -  Microscopy
KW  -  Fluorescence
KW  -  Pseudopodia
KW  -  User-Computer Interface
KW  -  Xenopus laevis
ID  - discovery10056832
N2  - Filopodia have important sensory and mechanical roles in motile cells. The recruitment of actin regulators, such as ENA/VASP proteins, to sites of protrusion underlies diverse molecular mechanisms of filopodia formation and extension. We developed Filopodyan (filopodia dynamics analysis) in Fiji and R to measure fluorescence in filopodia and at their tips and bases concurrently with their morphological and dynamic properties. Filopodyan supports high-throughput phenotype characterization as well as detailed interactive editing of filopodia reconstructions through an intuitive graphical user interface. Our highly customizable pipeline is widely applicable, capable of detecting filopodia in four different cell types in vitro and in vivo. We use Filopodyan to quantify the recruitment of ENA and VASP preceding filopodia formation in neuronal growth cones, and uncover a molecular heterogeneity whereby different filopodia display markedly different responses to changes in the accumulation of ENA and VASP fluorescence in their tips over time.
ER  -