TY - JOUR N2 - Positron emission tomography (PET) neuroimaging with the Pittsburgh Compound_B (PiB) is widely used to assess amyloid plaque burden. Standard quantification approaches normalize PiB-PET by mean cerebellar gray matter uptake. Previous studies suggested similar pons and white-matter uptake in Alzheimer's disease (AD) and healthy controls (HC), but lack exhaustive comparison of normalization across the three regions, with data-driven diagnostic classification. We aimed to compare the impact of distinct reference regions in normalization, measured by data-driven statistical analysis, and correlation with cerebrospinal fluid (CSF) amyloid ? (A?) species concentrations. 243 individuals with clinical diagnosis of AD, HC, mild cognitive impairment (MCI) and other dementias, from the Biomarkers for Alzheimer's/Parkinson's Disease (BIOMARKAPD) initiative were included. PiB-PET images and CSF concentrations of A?38, A?40 and A?42 were submitted to classification using support vector machines. Voxel-wise group differences and correlations between normalized PiB-PET images and CSF A? concentrations were calculated. Normalization by cerebellar gray matter and pons yielded identical classification accuracy of AD (accuracy-96%, sensitivity-96%, specificity-95%), and significantly higher than A? concentrations (best accuracy 91%). Normalization by the white-matter showed decreased extent of statistically significant multivoxel patterns and was the only method not outperforming CSF biomarkers, suggesting statistical inferiority. A?38 and A?40 correlated negatively with PiB-PET images normalized by the white-matter, corroborating previous observations of correlations with non-AD-specific subcortical changes in white-matter. In general, when using the pons as reference region, higher voxel-wise group differences and stronger correlation with A?42, the A?42/A?40 or A?42/A?38 ratios were found compared to normalization based on cerebellar gray matter. ID - discovery10056831 UR - http://dx.doi.org/10.1016/j.nicl.2018.08.023 SN - 2213-1582 JF - NeuroImage Clinical A1 - Oliveira, F A1 - Leuzy, A A1 - Castelhano, J A1 - Chiotis, K A1 - Hasselbalch, SG A1 - Rinne, J A1 - Mendonça, A A1 - Otto, M A1 - Lleó, A A1 - Santana, I A1 - Johansson, J A1 - Anderl-Straub, S A1 - Arnim, C A1 - Beer, A A1 - Blesa, R A1 - Fortea, J A1 - Sanna-Kaisa, H A1 - Portelius, E A1 - Pannee, J A1 - Zetterberg, H A1 - Blennow, K A1 - Moreira, AP A1 - Abrunhosa, A A1 - Nordberg, A A1 - Castelo-Branco, M TI - Data driven diagnostic classification in Alzheimer's disease based on different reference regions for normalization of PiB-PET images and correlation with CSF concentrations of A? species Y1 - 2018/// AV - public VL - 20 SP - 603 EP - 610 N1 - © 2018 The Authors. Published by Elsevier Inc. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/BY-NC-ND/4.0/). ER -