<> <http://www.w3.org/2000/01/rdf-schema#comment> "The repository administrator has not yet configured an RDF license."^^<http://www.w3.org/2001/XMLSchema#string> .
<> <http://xmlns.com/foaf/0.1/primaryTopic> <https://discovery.ucl.ac.uk/id/eprint/10055684> .
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<https://discovery.ucl.ac.uk/id/eprint/10055684> <http://www.w3.org/1999/02/22-rdf-syntax-ns#type> <http://purl.org/ontology/bibo/Article> .
<https://discovery.ucl.ac.uk/id/eprint/10055684> <http://purl.org/dc/terms/title> "Lentivector Producer Cell Lines with Stably Expressed Vesiculovirus Envelopes"^^<http://www.w3.org/2001/XMLSchema#string> .
<https://discovery.ucl.ac.uk/id/eprint/10055684> <http://purl.org/ontology/bibo/abstract> "Retroviral and lentiviral vectors often use the envelope G protein from the vesicular stomatitis virus Indiana strain (VSVind.G). However, lentivector producer cell lines that stably express VSVind.G have not been reported, presumably because of its cytotoxicity, preventing simple scale-up of vector production. Interestingly, we showed that VSVind.G and other vesiculovirus G from the VSV New Jersey strain (VSVnj), Cocal virus (COCV), and Piry virus (PIRYV) could be constitutively expressed and supported lentivector production for up to 10 weeks. All G-enveloped particles were robust, allowing concentration and freeze-thawing. COCV.G and PIRYV.G were resistant to complement inactivation, and, using chimeras between VSVind.G and COCV.G, the determinant for complement inactivation of VSVind.G was mapped to amino acid residues 136-370. Clonal packaging cell lines using COCV.G could be generated; however, during attempts to establish LV producer cells, vector superinfection was observed following the introduction of a lentivector genome. This could be prevented by culturing the cells with the antiviral drug nevirapine. As an alternative countermeasure, we demonstrated that functional lentivectors could be reconstituted by admixing supernatant from stable cells producing unenveloped virus with supernatant containing envelopes harvested from cells stably expressing VSVind.G, COCV.G, or PIRYV.G."^^<http://www.w3.org/2001/XMLSchema#string> .
<https://discovery.ucl.ac.uk/id/eprint/10055684> <http://purl.org/dc/terms/date> "2018-09-21" .
<https://discovery.ucl.ac.uk/id/document/738078> <http://www.w3.org/1999/02/22-rdf-syntax-ns#type> <http://purl.org/ontology/bibo/Document> .
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<https://discovery.ucl.ac.uk/id/publication/ext-23290501> <http://xmlns.com/foaf/0.1/name> "Molecular Therapy - Methods & Clinical Development"^^<http://www.w3.org/2001/XMLSchema#string> .
<https://discovery.ucl.ac.uk/id/eprint/10055684> <http://purl.org/dc/terms/isPartOf> <https://discovery.ucl.ac.uk/id/publication/ext-23290501> .
<https://discovery.ucl.ac.uk/id/publication/ext-23290501> <http://www.w3.org/2002/07/owl#sameAs> <urn:issn:23290501> .
<https://discovery.ucl.ac.uk/id/publication/ext-23290501> <http://purl.org/ontology/bibo/issn> "23290501" .
<https://discovery.ucl.ac.uk/id/eprint/10055684> <http://purl.org/ontology/bibo/status> <http://purl.org/ontology/bibo/status/published> .
<https://discovery.ucl.ac.uk/id/eprint/10055684> <http://purl.org/dc/terms/creator> <https://discovery.ucl.ac.uk/id/person/ext-6ed3bce9f7ceab5708ad3474672f5a73> .
<https://discovery.ucl.ac.uk/id/eprint/10055684> <http://purl.org/ontology/bibo/authorList> <https://discovery.ucl.ac.uk/id/eprint/10055684#authors> .
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<https://discovery.ucl.ac.uk/id/eprint/10055684> <http://purl.org/dc/terms/creator> <https://discovery.ucl.ac.uk/id/person/ext-9c25bedfd68a512f101b6248a57afcbd> .
<https://discovery.ucl.ac.uk/id/eprint/10055684> <http://purl.org/ontology/bibo/authorList> <https://discovery.ucl.ac.uk/id/eprint/10055684#authors> .
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<https://discovery.ucl.ac.uk/id/eprint/10055684> <http://purl.org/ontology/bibo/authorList> <https://discovery.ucl.ac.uk/id/eprint/10055684#authors> .
<https://discovery.ucl.ac.uk/id/eprint/10055684#authors> <http://www.w3.org/1999/02/22-rdf-syntax-ns#_3> <https://discovery.ucl.ac.uk/id/person/ext-3604ebc60c2f17839a741ae1dc98e4e3> .
<https://discovery.ucl.ac.uk/id/eprint/10055684> <http://purl.org/dc/terms/creator> <https://discovery.ucl.ac.uk/id/person/ext-c348c9dc12a09754fe4405744df5222e> .
<https://discovery.ucl.ac.uk/id/eprint/10055684> <http://purl.org/ontology/bibo/authorList> <https://discovery.ucl.ac.uk/id/eprint/10055684#authors> .
<https://discovery.ucl.ac.uk/id/eprint/10055684#authors> <http://www.w3.org/1999/02/22-rdf-syntax-ns#_4> <https://discovery.ucl.ac.uk/id/person/ext-c348c9dc12a09754fe4405744df5222e> .
<https://discovery.ucl.ac.uk/id/eprint/10055684> <http://purl.org/dc/terms/creator> <https://discovery.ucl.ac.uk/id/person/ext-3e009fddb391c936cfd711d51f531f3c> .
<https://discovery.ucl.ac.uk/id/eprint/10055684> <http://purl.org/ontology/bibo/authorList> <https://discovery.ucl.ac.uk/id/eprint/10055684#authors> .
<https://discovery.ucl.ac.uk/id/eprint/10055684#authors> <http://www.w3.org/1999/02/22-rdf-syntax-ns#_5> <https://discovery.ucl.ac.uk/id/person/ext-3e009fddb391c936cfd711d51f531f3c> .
<https://discovery.ucl.ac.uk/id/eprint/10055684> <http://purl.org/dc/terms/creator> <https://discovery.ucl.ac.uk/id/person/ext-970a0cfbb5c90c8219838e6530df4591> .
<https://discovery.ucl.ac.uk/id/eprint/10055684> <http://purl.org/ontology/bibo/authorList> <https://discovery.ucl.ac.uk/id/eprint/10055684#authors> .
<https://discovery.ucl.ac.uk/id/eprint/10055684#authors> <http://www.w3.org/1999/02/22-rdf-syntax-ns#_6> <https://discovery.ucl.ac.uk/id/person/ext-970a0cfbb5c90c8219838e6530df4591> .
<https://discovery.ucl.ac.uk/id/eprint/10055684> <http://purl.org/dc/terms/creator> <https://discovery.ucl.ac.uk/id/person/ext-3b30830f9baaa55bb942f7ea2876ef44> .
<https://discovery.ucl.ac.uk/id/eprint/10055684> <http://purl.org/ontology/bibo/authorList> <https://discovery.ucl.ac.uk/id/eprint/10055684#authors> .
<https://discovery.ucl.ac.uk/id/eprint/10055684#authors> <http://www.w3.org/1999/02/22-rdf-syntax-ns#_7> <https://discovery.ucl.ac.uk/id/person/ext-3b30830f9baaa55bb942f7ea2876ef44> .
<https://discovery.ucl.ac.uk/id/eprint/10055684> <http://purl.org/dc/terms/creator> <https://discovery.ucl.ac.uk/id/person/ext-40c5d8e7bc6891e5d6242846fef9042c> .
<https://discovery.ucl.ac.uk/id/eprint/10055684> <http://purl.org/ontology/bibo/authorList> <https://discovery.ucl.ac.uk/id/eprint/10055684#authors> .
<https://discovery.ucl.ac.uk/id/eprint/10055684#authors> <http://www.w3.org/1999/02/22-rdf-syntax-ns#_8> <https://discovery.ucl.ac.uk/id/person/ext-40c5d8e7bc6891e5d6242846fef9042c> .
<https://discovery.ucl.ac.uk/id/eprint/10055684> <http://purl.org/dc/terms/creator> <https://discovery.ucl.ac.uk/id/person/ext-d4869992b210869b755779da1edd6a06> .
<https://discovery.ucl.ac.uk/id/eprint/10055684> <http://purl.org/ontology/bibo/authorList> <https://discovery.ucl.ac.uk/id/eprint/10055684#authors> .
<https://discovery.ucl.ac.uk/id/eprint/10055684#authors> <http://www.w3.org/1999/02/22-rdf-syntax-ns#_9> <https://discovery.ucl.ac.uk/id/person/ext-d4869992b210869b755779da1edd6a06> .
<https://discovery.ucl.ac.uk/id/eprint/10055684> <http://purl.org/dc/terms/creator> <https://discovery.ucl.ac.uk/id/person/ext-c46b3c67d69b7c2195b83643eb767aa8> .
<https://discovery.ucl.ac.uk/id/eprint/10055684> <http://purl.org/ontology/bibo/authorList> <https://discovery.ucl.ac.uk/id/eprint/10055684#authors> .
<https://discovery.ucl.ac.uk/id/eprint/10055684#authors> <http://www.w3.org/1999/02/22-rdf-syntax-ns#_10> <https://discovery.ucl.ac.uk/id/person/ext-c46b3c67d69b7c2195b83643eb767aa8> .
<https://discovery.ucl.ac.uk/id/eprint/10055684> <http://purl.org/dc/terms/creator> <https://discovery.ucl.ac.uk/id/person/ext-81de7c6e29e423e900f8f5d37c184d49> .
<https://discovery.ucl.ac.uk/id/eprint/10055684> <http://purl.org/ontology/bibo/authorList> <https://discovery.ucl.ac.uk/id/eprint/10055684#authors> .
<https://discovery.ucl.ac.uk/id/eprint/10055684#authors> <http://www.w3.org/1999/02/22-rdf-syntax-ns#_11> <https://discovery.ucl.ac.uk/id/person/ext-81de7c6e29e423e900f8f5d37c184d49> .
<https://discovery.ucl.ac.uk/id/person/ext-40c5d8e7bc6891e5d6242846fef9042c> <http://www.w3.org/1999/02/22-rdf-syntax-ns#type> <http://xmlns.com/foaf/0.1/Person> .
<https://discovery.ucl.ac.uk/id/person/ext-40c5d8e7bc6891e5d6242846fef9042c> <http://xmlns.com/foaf/0.1/givenName> "I"^^<http://www.w3.org/2001/XMLSchema#string> .
<https://discovery.ucl.ac.uk/id/person/ext-40c5d8e7bc6891e5d6242846fef9042c> <http://xmlns.com/foaf/0.1/familyName> "Nisoli"^^<http://www.w3.org/2001/XMLSchema#string> .
<https://discovery.ucl.ac.uk/id/person/ext-40c5d8e7bc6891e5d6242846fef9042c> <http://xmlns.com/foaf/0.1/name> "I Nisoli"^^<http://www.w3.org/2001/XMLSchema#string> .
<https://discovery.ucl.ac.uk/id/person/ext-c46b3c67d69b7c2195b83643eb767aa8> <http://www.w3.org/1999/02/22-rdf-syntax-ns#type> <http://xmlns.com/foaf/0.1/Person> .
<https://discovery.ucl.ac.uk/id/person/ext-c46b3c67d69b7c2195b83643eb767aa8> <http://xmlns.com/foaf/0.1/givenName> "MK"^^<http://www.w3.org/2001/XMLSchema#string> .
<https://discovery.ucl.ac.uk/id/person/ext-c46b3c67d69b7c2195b83643eb767aa8> <http://xmlns.com/foaf/0.1/familyName> "Collins"^^<http://www.w3.org/2001/XMLSchema#string> .
<https://discovery.ucl.ac.uk/id/person/ext-c46b3c67d69b7c2195b83643eb767aa8> <http://xmlns.com/foaf/0.1/name> "MK Collins"^^<http://www.w3.org/2001/XMLSchema#string> .
<https://discovery.ucl.ac.uk/id/person/ext-970a0cfbb5c90c8219838e6530df4591> <http://www.w3.org/1999/02/22-rdf-syntax-ns#type> <http://xmlns.com/foaf/0.1/Person> .
<https://discovery.ucl.ac.uk/id/person/ext-970a0cfbb5c90c8219838e6530df4591> <http://xmlns.com/foaf/0.1/givenName> "T"^^<http://www.w3.org/2001/XMLSchema#string> .
<https://discovery.ucl.ac.uk/id/person/ext-970a0cfbb5c90c8219838e6530df4591> <http://xmlns.com/foaf/0.1/familyName> "Mukhopadhyay"^^<http://www.w3.org/2001/XMLSchema#string> .
<https://discovery.ucl.ac.uk/id/person/ext-970a0cfbb5c90c8219838e6530df4591> <http://xmlns.com/foaf/0.1/name> "T Mukhopadhyay"^^<http://www.w3.org/2001/XMLSchema#string> .
<https://discovery.ucl.ac.uk/id/person/ext-3604ebc60c2f17839a741ae1dc98e4e3> <http://www.w3.org/1999/02/22-rdf-syntax-ns#type> <http://xmlns.com/foaf/0.1/Person> .
<https://discovery.ucl.ac.uk/id/person/ext-3604ebc60c2f17839a741ae1dc98e4e3> <http://xmlns.com/foaf/0.1/givenName> "C"^^<http://www.w3.org/2001/XMLSchema#string> .
<https://discovery.ucl.ac.uk/id/person/ext-3604ebc60c2f17839a741ae1dc98e4e3> <http://xmlns.com/foaf/0.1/familyName> "Perry"^^<http://www.w3.org/2001/XMLSchema#string> .
<https://discovery.ucl.ac.uk/id/person/ext-3604ebc60c2f17839a741ae1dc98e4e3> <http://xmlns.com/foaf/0.1/name> "C Perry"^^<http://www.w3.org/2001/XMLSchema#string> .
<https://discovery.ucl.ac.uk/id/person/ext-6ed3bce9f7ceab5708ad3474672f5a73> <http://www.w3.org/1999/02/22-rdf-syntax-ns#type> <http://xmlns.com/foaf/0.1/Person> .
<https://discovery.ucl.ac.uk/id/person/ext-6ed3bce9f7ceab5708ad3474672f5a73> <http://xmlns.com/foaf/0.1/givenName> "M"^^<http://www.w3.org/2001/XMLSchema#string> .
<https://discovery.ucl.ac.uk/id/person/ext-6ed3bce9f7ceab5708ad3474672f5a73> <http://xmlns.com/foaf/0.1/familyName> "Tijani"^^<http://www.w3.org/2001/XMLSchema#string> .
<https://discovery.ucl.ac.uk/id/person/ext-6ed3bce9f7ceab5708ad3474672f5a73> <http://xmlns.com/foaf/0.1/name> "M Tijani"^^<http://www.w3.org/2001/XMLSchema#string> .
<https://discovery.ucl.ac.uk/id/person/ext-81de7c6e29e423e900f8f5d37c184d49> <http://www.w3.org/1999/02/22-rdf-syntax-ns#type> <http://xmlns.com/foaf/0.1/Person> .
<https://discovery.ucl.ac.uk/id/person/ext-81de7c6e29e423e900f8f5d37c184d49> <http://xmlns.com/foaf/0.1/givenName> "Y"^^<http://www.w3.org/2001/XMLSchema#string> .
<https://discovery.ucl.ac.uk/id/person/ext-81de7c6e29e423e900f8f5d37c184d49> <http://xmlns.com/foaf/0.1/familyName> "Takeuchi"^^<http://www.w3.org/2001/XMLSchema#string> .
<https://discovery.ucl.ac.uk/id/person/ext-81de7c6e29e423e900f8f5d37c184d49> <http://xmlns.com/foaf/0.1/name> "Y Takeuchi"^^<http://www.w3.org/2001/XMLSchema#string> .
<https://discovery.ucl.ac.uk/id/person/ext-3b30830f9baaa55bb942f7ea2876ef44> <http://www.w3.org/1999/02/22-rdf-syntax-ns#type> <http://xmlns.com/foaf/0.1/Person> .
<https://discovery.ucl.ac.uk/id/person/ext-3b30830f9baaa55bb942f7ea2876ef44> <http://xmlns.com/foaf/0.1/givenName> "M"^^<http://www.w3.org/2001/XMLSchema#string> .
<https://discovery.ucl.ac.uk/id/person/ext-3b30830f9baaa55bb942f7ea2876ef44> <http://xmlns.com/foaf/0.1/familyName> "Themis"^^<http://www.w3.org/2001/XMLSchema#string> .
<https://discovery.ucl.ac.uk/id/person/ext-3b30830f9baaa55bb942f7ea2876ef44> <http://xmlns.com/foaf/0.1/name> "M Themis"^^<http://www.w3.org/2001/XMLSchema#string> .
<https://discovery.ucl.ac.uk/id/person/ext-d4869992b210869b755779da1edd6a06> <http://www.w3.org/1999/02/22-rdf-syntax-ns#type> <http://xmlns.com/foaf/0.1/Person> .
<https://discovery.ucl.ac.uk/id/person/ext-d4869992b210869b755779da1edd6a06> <http://xmlns.com/foaf/0.1/givenName> "G"^^<http://www.w3.org/2001/XMLSchema#string> .
<https://discovery.ucl.ac.uk/id/person/ext-d4869992b210869b755779da1edd6a06> <http://xmlns.com/foaf/0.1/familyName> "Mattiuzzo"^^<http://www.w3.org/2001/XMLSchema#string> .
<https://discovery.ucl.ac.uk/id/person/ext-d4869992b210869b755779da1edd6a06> <http://xmlns.com/foaf/0.1/name> "G Mattiuzzo"^^<http://www.w3.org/2001/XMLSchema#string> .
<https://discovery.ucl.ac.uk/id/person/ext-3e009fddb391c936cfd711d51f531f3c> <http://www.w3.org/1999/02/22-rdf-syntax-ns#type> <http://xmlns.com/foaf/0.1/Person> .
<https://discovery.ucl.ac.uk/id/person/ext-3e009fddb391c936cfd711d51f531f3c> <http://xmlns.com/foaf/0.1/givenName> "M"^^<http://www.w3.org/2001/XMLSchema#string> .
<https://discovery.ucl.ac.uk/id/person/ext-3e009fddb391c936cfd711d51f531f3c> <http://xmlns.com/foaf/0.1/familyName> "Ferraresso"^^<http://www.w3.org/2001/XMLSchema#string> .
<https://discovery.ucl.ac.uk/id/person/ext-3e009fddb391c936cfd711d51f531f3c> <http://xmlns.com/foaf/0.1/name> "M Ferraresso"^^<http://www.w3.org/2001/XMLSchema#string> .
<https://discovery.ucl.ac.uk/id/person/ext-c348c9dc12a09754fe4405744df5222e> <http://www.w3.org/1999/02/22-rdf-syntax-ns#type> <http://xmlns.com/foaf/0.1/Person> .
<https://discovery.ucl.ac.uk/id/person/ext-c348c9dc12a09754fe4405744df5222e> <http://xmlns.com/foaf/0.1/givenName> "K"^^<http://www.w3.org/2001/XMLSchema#string> .
<https://discovery.ucl.ac.uk/id/person/ext-c348c9dc12a09754fe4405744df5222e> <http://xmlns.com/foaf/0.1/familyName> "Sanber"^^<http://www.w3.org/2001/XMLSchema#string> .
<https://discovery.ucl.ac.uk/id/person/ext-c348c9dc12a09754fe4405744df5222e> <http://xmlns.com/foaf/0.1/name> "K Sanber"^^<http://www.w3.org/2001/XMLSchema#string> .
<https://discovery.ucl.ac.uk/id/person/ext-9c25bedfd68a512f101b6248a57afcbd> <http://www.w3.org/1999/02/22-rdf-syntax-ns#type> <http://xmlns.com/foaf/0.1/Person> .
<https://discovery.ucl.ac.uk/id/person/ext-9c25bedfd68a512f101b6248a57afcbd> <http://xmlns.com/foaf/0.1/givenName> "AM"^^<http://www.w3.org/2001/XMLSchema#string> .
<https://discovery.ucl.ac.uk/id/person/ext-9c25bedfd68a512f101b6248a57afcbd> <http://xmlns.com/foaf/0.1/familyName> "Munis"^^<http://www.w3.org/2001/XMLSchema#string> .
<https://discovery.ucl.ac.uk/id/person/ext-9c25bedfd68a512f101b6248a57afcbd> <http://xmlns.com/foaf/0.1/name> "AM Munis"^^<http://www.w3.org/2001/XMLSchema#string> .
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<https://discovery.ucl.ac.uk/id/eprint/10055684> <http://www.w3.org/1999/02/22-rdf-syntax-ns#type> <http://eprints.org/ontology/ArticleEPrint> .
<https://discovery.ucl.ac.uk/id/eprint/10055684> <http://purl.org/dc/terms/isPartOf> <https://discovery.ucl.ac.uk/id/repository> .
<https://discovery.ucl.ac.uk/id/eprint/10055684> <http://eprints.org/ontology/hasDocument> <https://discovery.ucl.ac.uk/id/document/738078> .
<https://discovery.ucl.ac.uk/id/document/738078> <http://www.w3.org/1999/02/22-rdf-syntax-ns#type> <http://eprints.org/ontology/Document> .
<https://discovery.ucl.ac.uk/id/document/738078> <http://www.w3.org/2000/01/rdf-schema#label> "Lentivector Producer Cell Lines with Stably Expressed Vesiculovirus Envelopes (Text)"^^<http://www.w3.org/2001/XMLSchema#string> .
<https://discovery.ucl.ac.uk/id/eprint/10055684> <http://purl.org/dc/elements/1.1/hasVersion> <https://discovery.ucl.ac.uk/id/document/738078> .
<https://discovery.ucl.ac.uk/id/eprint/10055684> <http://eprints.org/ontology/hasPublished> <https://discovery.ucl.ac.uk/id/document/738078> .
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<https://discovery.ucl.ac.uk/id/document/738078> <http://purl.org/dc/terms/hasPart> <https://discovery.ucl.ac.uk/id/eprint/10055684/1/Tijani_Lentivector.pdf> .
<https://discovery.ucl.ac.uk/id/eprint/10055684/1/Tijani_Lentivector.pdf> <http://www.w3.org/2000/01/rdf-schema#label> "Tijani_Lentivector.pdf"^^<http://www.w3.org/2001/XMLSchema#string> .
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