eprintid: 10053413 rev_number: 23 eprint_status: archive userid: 608 dir: disk0/10/05/34/13 datestamp: 2018-08-02 11:51:11 lastmod: 2020-02-13 03:29:48 status_changed: 2018-08-02 11:51:11 type: article metadata_visibility: show creators_name: Ermine, CM creators_name: Wright, JL creators_name: Parish, CL creators_name: Stanic, D creators_name: Thompson, LH title: Combined immunohistochemical and retrograde tracing reveals little evidence of innervation of the rat dentate gyrus by midbrain dopamine neurons ispublished: pub divisions: UCL divisions: A01 divisions: B02 divisions: C10 divisions: D17 divisions: G95 keywords: noradrenaline, hippocampus, connectivity, DAT, neurogenesis note: This version is the author accepted manuscript. For information on re-use, please refer to the publisher’s terms and conditions. abstract: Although the functional neuroanatomy of the midbrain dopamine (mDA) system has been well characterized, the literature regarding its capacity to innervate the hippocampal formation has been inconsistent. The lack of expression of definitive markers for dopaminergic fibers, such as the dopamine transporter, in the hippocampus has complicated studies in this area. Here we have used immunohistochemical techniques to characterize the tyrosine hydroxylase expressing fiber network in the rat hippocampus, combined with retrograde tracing from the dentate gyrus to assess the capacity for afferent innervation by mDA neurons. The results indicate that virtually all tyrosine hydroxylase fibers throughout the hippocampus are of a noradrenergic phenotype, while the overlying cortex contains both dopaminergic and noradrenergic fiber networks. Furthermore, retrograde tracing from the dentate gyrus robustly labels tyrosine hydroxylase-immunoreactive noradrenergic neurons in the locus coeruleus but not mDA neurons. date: 2016-06 date_type: published official_url: http://dx.doi.org/10.1007/s11515-016-1404-4 oa_status: green full_text_type: other language: eng primo: open primo_central: open_green verified: verified_manual elements_id: 1535082 doi: 10.1007/s11515-016-1404-4 lyricists_name: Wright, Jordan lyricists_id: JWRIG46 actors_name: Allington-Smith, Dominic actors_id: DAALL44 actors_role: owner full_text_status: public publication: Frontiers in Biology volume: 11 number: 3 pagerange: 246-255 issn: 1674-7992 citation: Ermine, CM; Wright, JL; Parish, CL; Stanic, D; Thompson, LH; (2016) Combined immunohistochemical and retrograde tracing reveals little evidence of innervation of the rat dentate gyrus by midbrain dopamine neurons. Frontiers in Biology , 11 (3) pp. 246-255. 10.1007/s11515-016-1404-4 <https://doi.org/10.1007/s11515-016-1404-4>. Green open access document_url: https://discovery.ucl.ac.uk/id/eprint/10053413/1/Wright_Combined%20immunohistochemical%20and%20retrograde.pdf