<> <http://www.w3.org/2000/01/rdf-schema#comment> "The repository administrator has not yet configured an RDF license."^^<http://www.w3.org/2001/XMLSchema#string> . <> <http://xmlns.com/foaf/0.1/primaryTopic> <https://discovery.ucl.ac.uk/id/eprint/10052362> . <https://discovery.ucl.ac.uk/id/eprint/10052362> <http://www.w3.org/1999/02/22-rdf-syntax-ns#type> <http://purl.org/ontology/bibo/AcademicArticle> . <https://discovery.ucl.ac.uk/id/eprint/10052362> <http://www.w3.org/1999/02/22-rdf-syntax-ns#type> <http://purl.org/ontology/bibo/Article> . <https://discovery.ucl.ac.uk/id/eprint/10052362> <http://purl.org/dc/terms/title> "Genome-Wide Posttranscriptional Dysregulation by MicroRNAs in Human Asthma as Revealed by Frac-seq"^^<http://www.w3.org/2001/XMLSchema#string> . <https://discovery.ucl.ac.uk/id/eprint/10052362> <http://purl.org/ontology/bibo/abstract> "MicroRNAs are small noncoding RNAs that inhibit gene expression posttranscriptionally, implicated in virtually all biological processes. Although the effect of individual microRNAs is generally studied, the genome-wide role of multiple microRNAs is less investigated. We assessed paired genome-wide expression of microRNAs with total (cytoplasmic) and translational (polyribosome-bound) mRNA levels employing subcellular fractionation and RNA sequencing (Frac-seq) in human primary bronchoepithelium from healthy controls and severe asthmatics. Severe asthma is a chronic inflammatory disease of the airways characterized by poor response to therapy. We found genes (i.e., isoforms of a gene) and mRNA isoforms differentially expressed in asthma, with novel inflammatory and structural pathophysiological mechanisms related to bronchoepithelium disclosed solely by polyribosome-bound mRNAs (e.g., IL1A and LTB genes or ITGA6 and ITGA2 alternatively spliced isoforms). Gene expression (i.e., isoforms of a gene) and mRNA expression analysis revealed different molecular candidates and biological pathways, with differentially expressed polyribosome-bound and total mRNAs also showing little overlap. We reveal a hub of six dysregulated microRNAs accounting for ∼90% of all microRNA targeting, displaying preference for polyribosome-bound mRNAs. Transfection of this hub in bronchial epithelial cells from healthy donors mimicked asthma characteristics. Our work demonstrates extensive posttranscriptional gene dysregulation in human asthma, in which microRNAs play a central role, illustrating the feasibility and importance of assessing posttranscriptional gene expression when investigating human disease."^^<http://www.w3.org/2001/XMLSchema#string> . <https://discovery.ucl.ac.uk/id/eprint/10052362> <http://purl.org/dc/terms/date> "2018-07-01" . <https://discovery.ucl.ac.uk/id/document/700569> <http://www.w3.org/1999/02/22-rdf-syntax-ns#type> <http://purl.org/ontology/bibo/Document> . <https://discovery.ucl.ac.uk/id/eprint/10052362> <http://purl.org/ontology/bibo/volume> "201" . <https://discovery.ucl.ac.uk/id/eprint/10052362> <http://purl.org/ontology/bibo/issue> "1" . <https://discovery.ucl.ac.uk/id/publication/ext-15506606> <http://www.w3.org/1999/02/22-rdf-syntax-ns#type> <http://purl.org/ontology/bibo/Collection> . <https://discovery.ucl.ac.uk/id/publication/ext-15506606> <http://xmlns.com/foaf/0.1/name> "The Journal of 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<https://discovery.ucl.ac.uk/id/person/ext-da356cd4174b7162e8f21f494f42b4f9> . <https://discovery.ucl.ac.uk/id/eprint/10052362> <http://purl.org/dc/terms/creator> <https://discovery.ucl.ac.uk/id/person/ext-a64f74ec1a8dc7be24ed174997c54c69> . <https://discovery.ucl.ac.uk/id/eprint/10052362> <http://purl.org/ontology/bibo/authorList> <https://discovery.ucl.ac.uk/id/eprint/10052362#authors> . <https://discovery.ucl.ac.uk/id/eprint/10052362#authors> <http://www.w3.org/1999/02/22-rdf-syntax-ns#_4> <https://discovery.ucl.ac.uk/id/person/ext-a64f74ec1a8dc7be24ed174997c54c69> . <https://discovery.ucl.ac.uk/id/eprint/10052362> <http://purl.org/dc/terms/creator> <https://discovery.ucl.ac.uk/id/person/ext-bbde8770135f01cfe1d68f632e074a6b> . <https://discovery.ucl.ac.uk/id/eprint/10052362> <http://purl.org/ontology/bibo/authorList> <https://discovery.ucl.ac.uk/id/eprint/10052362#authors> . <https://discovery.ucl.ac.uk/id/eprint/10052362#authors> <http://www.w3.org/1999/02/22-rdf-syntax-ns#_5> 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<https://discovery.ucl.ac.uk/id/person/ext-a64f74ec1a8dc7be24ed174997c54c69> <http://xmlns.com/foaf/0.1/name> "M Niranjan"^^<http://www.w3.org/2001/XMLSchema#string> . <https://discovery.ucl.ac.uk/id/person/ext-097a46ce63db96a2657b69ed57172737> <http://www.w3.org/1999/02/22-rdf-syntax-ns#type> <http://xmlns.com/foaf/0.1/Person> . <https://discovery.ucl.ac.uk/id/person/ext-097a46ce63db96a2657b69ed57172737> <http://xmlns.com/foaf/0.1/givenName> "RT"^^<http://www.w3.org/2001/XMLSchema#string> . <https://discovery.ucl.ac.uk/id/person/ext-097a46ce63db96a2657b69ed57172737> <http://xmlns.com/foaf/0.1/familyName> "Martinez-Nunez"^^<http://www.w3.org/2001/XMLSchema#string> . <https://discovery.ucl.ac.uk/id/person/ext-097a46ce63db96a2657b69ed57172737> <http://xmlns.com/foaf/0.1/name> "RT Martinez-Nunez"^^<http://www.w3.org/2001/XMLSchema#string> . <https://discovery.ucl.ac.uk/id/person/ext-5f022e13b959716f828f13d292447e9b> <http://www.w3.org/1999/02/22-rdf-syntax-ns#type> 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