eprintid: 10051550
rev_number: 37
eprint_status: archive
userid: 608
dir: disk0/10/05/15/50
datestamp: 2018-11-22 17:45:59
lastmod: 2021-10-04 00:52:14
status_changed: 2018-11-22 17:45:59
type: article
metadata_visibility: show
creators_name: Novakova, L
creators_name: Singh, AK
creators_name: Axelsson, M
creators_name: Ståhlman, M
creators_name: Adiels, M
creators_name: Malmeström, C
creators_name: Zetterberg, H
creators_name: Borén, J
creators_name: Lycke, J
creators_name: Cardell, SL
creators_name: Blomqvist, M
title: Sulfatide isoform pattern in cerebrospinal fluid discriminates progressive MS from relapsing‐remitting MS
ispublished: pub
divisions: UCL
divisions: B02
divisions: C07
divisions: D07
divisions: F86
keywords: axonal loss, biomarkers, cerebrospinal fluid, demyelination, inflammation, mass spectrometry, multiple sclerosis, sulfatide
note: This version is the author accepted manuscript. For information on re-use, please refer to the publisher’s terms and conditions.
abstract: Multiple sclerosis (MS) is an inflammatory demyelinating disease of the central nervous system (CNS). Several biomarkers including proteins and lipids have been reported in MS cerebrospinal fluid (CSF), reflecting different aspects of the pathophysiology particularly of relapsing‐remitting MS (RRMS). Sulfatide, abundant in the myelin sheath and a proposed target for autoimmune attack in MS, has been reported altered in MS CSF. Here, we investigated the potential of CSF sulfatide and its isoforms as biomarkers in MS. A highly sensitive and quantitative mass spectrometry method was employed to determine levels of sulfatide isoforms in CSF from RRMS and progressive MS (PMS) patients, and healthy donors (HD). We demonstrate that levels of total CSF sulfatide and C24:1, C26:1, and C26:1‐OH isoforms were significantly increased in PMS compared with RRMS patients and HD, while C23:0‐OH was significantly decreased in CSF from PMS patients compared to the other two groups. Multivariate discriminant analysis showed that CSF sulfatide isoform pattern in PMS patients was distinct and non‐overlapping with that of RRMS patients and HD. Sulfatide levels did not correlate with tested biomarkers or clinical parameters. The results suggest that CSF sulfatide isoform levels may be used to discriminate the phenotype of MS and might play a role in the progression of the disease.
date: 2018-08
date_type: published
publisher: WILEY
official_url: https://doi.org/10.1111/jnc.14452
oa_status: green
full_text_type: other
language: eng
primo: open
primo_central: open_green
verified: verified_manual
elements_id: 1553519
doi: 10.1111/jnc.14452
lyricists_name: Zetterberg, Henrik
lyricists_id: HZETT94
actors_name: Allington-Smith, Dominic
actors_id: DAALL44
actors_role: owner
full_text_status: public
publication: Journal of Neurochemistry
volume: 146
number: 3
pagerange: 322-332
pages: 11
issn: 1471-4159
citation:        Novakova, L;    Singh, AK;    Axelsson, M;    Ståhlman, M;    Adiels, M;    Malmeström, C;    Zetterberg, H;                 ... Blomqvist, M; + view all <#>        Novakova, L;  Singh, AK;  Axelsson, M;  Ståhlman, M;  Adiels, M;  Malmeström, C;  Zetterberg, H;  Borén, J;  Lycke, J;  Cardell, SL;  Blomqvist, M;   - view fewer <#>    (2018)    Sulfatide isoform pattern in cerebrospinal fluid discriminates progressive MS from relapsing‐remitting MS.                   Journal of Neurochemistry , 146  (3)   pp. 322-332.    10.1111/jnc.14452 <https://doi.org/10.1111/jnc.14452>.       Green open access   
 
document_url: https://discovery.ucl.ac.uk/id/eprint/10051550/1/Zetterberg_171222e%20Sulf%20CSF_HZ.pdf