eprintid: 10051349
rev_number: 24
eprint_status: archive
userid: 608
dir: disk0/10/05/13/49
datestamp: 2018-07-02 13:26:23
lastmod: 2021-09-28 22:25:15
status_changed: 2018-07-02 13:26:23
type: article
metadata_visibility: show
creators_name: van Raaij, S
creators_name: van Swelm, R
creators_name: Bouman, K
creators_name: Cliteur, M
creators_name: van den Heuvel, M
creators_name: Pertijs, J
creators_name: Patel, D
creators_name: Bass, P
creators_name: van Goor, H
creators_name: Unwin, R
creators_name: Srai, SK
creators_name: Swinkels, D
title: Tubular iron deposition and iron handling proteins in human healthy kidney and chronic kidney disease
ispublished: pub
divisions: UCL
divisions: B02
divisions: C08
divisions: D09
divisions: G03
divisions: C10
divisions: D19
divisions: G97
divisions: D17
note: Copyright © The Author(s) 2018. Open Access: This article is licensed under a Creative Commons Attribution 4.0 International
License, which permits use, sharing, adaptation, distribution and reproduction in any medium or
format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative
Commons license, and indicate if changes were made. Te images or other third party material in this
article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the
material. If material is not included in the article’s Creative Commons license and your intended use is not permitted
by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the
copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
abstract: Iron is suggested to play a detrimental role in the progression of chronic kidney disease (CKD). The kidney recycles iron back into the circulation. However, the localization of proteins relevant for physiological tubular iron handling and their potential role in CKD remain unclear. We examined associations between iron deposition, expression of iron handling proteins and tubular injury in kidney biopsies from CKD patients and healthy controls using immunohistochemistry. Iron was deposited in proximal (PT) and distal tubules (DT) in 33% of CKD biopsies, predominantly in pathologies with glomerular dysfunction, but absent in controls. In healthy kidney, PT contained proteins required for iron recycling including putative iron importers ZIP8, ZIP14, DMT1, iron storage proteins L- and H-ferritin and iron exporter ferroportin, while DT only contained ZIP8, ZIP14, and DMT1. In CKD, iron deposition associated with increased intensity of iron importers (ZIP14, ZIP8), storage proteins (L-, H-ferritin), and/or decreased ferroportin abundance. This demonstrates that tubular iron accumulation may result from increased iron uptake and/or inadequate iron export. Iron deposition associated with oxidative injury as indicated by heme oxygenase-1 abundance. In conclusion, iron deposition is relatively common in CKD, and may result from altered molecular iron handling and may contribute to renal injury.
date: 2018-06
date_type: published
official_url: https://doi.org/10.1038/s41598-018-27107-8
oa_status: green
full_text_type: pub
language: eng
primo: open
primo_central: open_green
article_type_text: Journal Article
verified: verified_manual
elements_id: 1563815
doi: 10.1038/s41598-018-27107-8
pii: 10.1038/s41598-018-27107-8
lyricists_name: Bass, Paul
lyricists_name: Patel, Dominic
lyricists_name: Srai, Surjit
lyricists_name: Unwin, Robert
lyricists_id: PBASS04
lyricists_id: PATEL39
lyricists_id: SKSSR22
lyricists_id: RJUNW23
actors_name: Cuccu, Clara
actors_id: CCCUC40
actors_role: owner
full_text_status: public
publication: Scientific Reports
volume: 8
article_number: 9353
event_location: England
issn: 2045-2322
citation:        van Raaij, S;    van Swelm, R;    Bouman, K;    Cliteur, M;    van den Heuvel, M;    Pertijs, J;    Patel, D;                     ... Swinkels, D; + view all <#>        van Raaij, S;  van Swelm, R;  Bouman, K;  Cliteur, M;  van den Heuvel, M;  Pertijs, J;  Patel, D;  Bass, P;  van Goor, H;  Unwin, R;  Srai, SK;  Swinkels, D;   - view fewer <#>    (2018)    Tubular iron deposition and iron handling proteins in human healthy kidney and chronic kidney disease.                   Scientific Reports , 8     , Article 9353.  10.1038/s41598-018-27107-8 <https://doi.org/10.1038/s41598-018-27107-8>.       Green open access   
 
document_url: https://discovery.ucl.ac.uk/id/eprint/10051349/1/van%20Raaij1_Tubular.pdf
document_url: https://discovery.ucl.ac.uk/id/eprint/10051349/8/41598_2018_27107_MOESM1_ESM.pdf