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<https://discovery.ucl.ac.uk/id/eprint/10050639> <http://www.w3.org/1999/02/22-rdf-syntax-ns#type> <http://purl.org/ontology/bibo/Article> .
<https://discovery.ucl.ac.uk/id/eprint/10050639> <http://purl.org/dc/terms/title> "Genome-Wide Transcriptome Analysis of CD36 Overexpression in HepG2.2.15 Cells to Explore Its Regulatory Role in Metabolism and the Hepatitis B Virus Life Cycle"^^<http://www.w3.org/2001/XMLSchema#string> .
<https://discovery.ucl.ac.uk/id/eprint/10050639> <http://purl.org/ontology/bibo/abstract> "Hepatitis B virus (HBV) is a hepatocyte-specific DNA virus whose gene expression and replication\r\nare closely associated with hepatic metabolic processes. Thus, a potential anti-viral\r\nstrategy is to target the host metabolic factors necessary for HBV gene expression and replication.\r\nRecent studies revealed that fatty acid translocase CD36 is involved in the replication,\r\nassembly, storage, and secretion of certain viruses, such as hepatitis C virus (HCV)\r\nand human immunodeficiency virus (HIV). However, the relationship between CD36 and\r\nthe HBV life cycle remains unclear. Here, we showed, for the first time, that increased\r\nCD36 expression enhances HBV replication in HepG2.2.15 cells. To understand the underlying\r\nmolecular basis, we performed genome-wide sequencing of the mRNA from\r\nHepG2.2.15-CD36 overexpression (CD36OE) cells and HepG2.2.15-vector cells using\r\nRNA Sequencing (RNA-seq) technology to analyze the differential transcriptomic profile.\r\nOur results identified 141 differentially expressed genes (DEGs) related to CD36 overexpression,\r\nincluding 79 upregulated genes and 62 downregulated genes. Gene ontology\r\nand KEGG pathway analysis revealed that some of the DEGs were involved in various metabolic\r\nprocesses and the HBV life cycle. The reliability of the RNA-Seq data was confirmed\r\nby qPCR analysis. Our findings provide clues to build a link between CD36, host metabolism\r\nand the HBV life cycle and identified areas that require further investigation."^^<http://www.w3.org/2001/XMLSchema#string> .
<https://discovery.ucl.ac.uk/id/eprint/10050639> <http://purl.org/dc/terms/date> "2016-10-17" .
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<https://discovery.ucl.ac.uk/id/person/ext-439ec5e1633367628251ad3e7c24ce87> <http://xmlns.com/foaf/0.1/givenName> "J"^^<http://www.w3.org/2001/XMLSchema#string> .
<https://discovery.ucl.ac.uk/id/person/ext-439ec5e1633367628251ad3e7c24ce87> <http://xmlns.com/foaf/0.1/familyName> "Huang"^^<http://www.w3.org/2001/XMLSchema#string> .
<https://discovery.ucl.ac.uk/id/person/ext-439ec5e1633367628251ad3e7c24ce87> <http://xmlns.com/foaf/0.1/name> "J Huang"^^<http://www.w3.org/2001/XMLSchema#string> .
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<https://discovery.ucl.ac.uk/id/person/ext-fc909ab0e7afeb4df99cddd0619d091c> <http://xmlns.com/foaf/0.1/familyName> "Chen"^^<http://www.w3.org/2001/XMLSchema#string> .
<https://discovery.ucl.ac.uk/id/person/ext-fc909ab0e7afeb4df99cddd0619d091c> <http://xmlns.com/foaf/0.1/name> "Z Chen"^^<http://www.w3.org/2001/XMLSchema#string> .
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<https://discovery.ucl.ac.uk/id/person/ext-971febce56c43422db917d3a355ea80c> <http://xmlns.com/foaf/0.1/familyName> "Tang"^^<http://www.w3.org/2001/XMLSchema#string> .
<https://discovery.ucl.ac.uk/id/person/ext-971febce56c43422db917d3a355ea80c> <http://xmlns.com/foaf/0.1/name> "N Tang"^^<http://www.w3.org/2001/XMLSchema#string> .
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<https://discovery.ucl.ac.uk/id/person/ext-8e48c6d37be74596003fb305bd5760af> <http://xmlns.com/foaf/0.1/givenName> "P"^^<http://www.w3.org/2001/XMLSchema#string> .
<https://discovery.ucl.ac.uk/id/person/ext-8e48c6d37be74596003fb305bd5760af> <http://xmlns.com/foaf/0.1/familyName> "Yang"^^<http://www.w3.org/2001/XMLSchema#string> .
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<https://discovery.ucl.ac.uk/id/person/ext-4f4387d7b65c97d2ac38bd96fc2eb000> <http://xmlns.com/foaf/0.1/name> "L Zhao"^^<http://www.w3.org/2001/XMLSchema#string> .
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