TY  - JOUR
SP  - 3
EP  - 15
SN  - 1940-6029
A1  - Mirsky, R
A1  - Jessen, KR
N2  - Schwann cell precursors are the first defined stage in the generation of Schwann cells from the neural crest and represent the glial cell of embryonic nerves. Highly pure cultures of these cells can be obtained by enzymatic dissociation of nerves dissected from the limbs of 14- or 12-day-old rat and mouse embryos, respectively. Since Schwann cell precursors, unlike Schwann cells, are acutely dependent on axonal signals for survival, they require addition of trophic factors, typically ?-neuregulin-1, for maintenance in cell culture. Under these conditions they convert to Schwann cells on schedule, within about 4 days.
KW  - Nerve development
KW  -  Neural crest
KW  -  Neuregulin
KW  -  Precursors
KW  -  Schwann cells
AV  - public
TI  - Isolation of Schwann Cell Precursors from Rodents.
N1  - This version is the author accepted manuscript. For information on re-use, please refer to the publisher?s terms and conditions.
ID  - discovery10048371
Y1  - 2018///
UR  - https://doi.org/10.1007/978-1-4939-7649-2_1
JF  - Methods in Molecular Biology
VL  - 1739
ER  -