TY - JOUR SP - 3 EP - 15 SN - 1940-6029 A1 - Mirsky, R A1 - Jessen, KR N2 - Schwann cell precursors are the first defined stage in the generation of Schwann cells from the neural crest and represent the glial cell of embryonic nerves. Highly pure cultures of these cells can be obtained by enzymatic dissociation of nerves dissected from the limbs of 14- or 12-day-old rat and mouse embryos, respectively. Since Schwann cell precursors, unlike Schwann cells, are acutely dependent on axonal signals for survival, they require addition of trophic factors, typically ?-neuregulin-1, for maintenance in cell culture. Under these conditions they convert to Schwann cells on schedule, within about 4 days. KW - Nerve development KW - Neural crest KW - Neuregulin KW - Precursors KW - Schwann cells AV - public TI - Isolation of Schwann Cell Precursors from Rodents. N1 - This version is the author accepted manuscript. For information on re-use, please refer to the publisher?s terms and conditions. ID - discovery10048371 Y1 - 2018/// UR - https://doi.org/10.1007/978-1-4939-7649-2_1 JF - Methods in Molecular Biology VL - 1739 ER -