eprintid: 10045875
rev_number: 19
eprint_status: archive
userid: 608
dir: disk0/10/04/58/75
datestamp: 2018-03-28 13:45:39
lastmod: 2021-09-17 22:13:52
status_changed: 2018-03-28 13:45:39
type: article
metadata_visibility: show
creators_name: von Erlach, TC
creators_name: Bertazzo, S
creators_name: Wozniak, MA
creators_name: Horejs, C-M
creators_name: Maynard, SA
creators_name: Attwood, S
creators_name: Robinson, BK
creators_name: Autefage, H
creators_name: Kallepitis, C
creators_name: Hernandez, ADR
creators_name: Chen, CS
creators_name: Goldoni, S
creators_name: Stevens, MM
title: Cell-geometry-dependent changes in plasma membrane order direct stem cell signalling and fate
ispublished: pub
divisions: UCL
divisions: B04
divisions: C05
divisions: F42
keywords: Science & Technology, Physical Sciences, Technology, Chemistry, Physical, Materials Science, Multidisciplinary, Physics, Applied, Physics, Condensed Matter, Chemistry, Materials Science, Physics, LIPID RAFTS, CAVEOLAE, MICROTUBULES, MICRODOMAINS, GROWTH, SWITCH
note: This version is the author accepted manuscript. For information on re-use, please refer to the publisher’s terms and conditions.
abstract: Cell size and shape affect cellular processes such as cell survival, growth and differentiation1,2,3,4, thus establishing cell geometry as a fundamental regulator of cell physiology. The contributions of the cytoskeleton, specifically actomyosin tension, to these effects have been described, but the exact biophysical mechanisms that translate changes in cell geometry to changes in cell behaviour remain mostly unresolved. Using a variety of innovative materials techniques, we demonstrate that the nanostructure and lipid assembly within the cell plasma membrane are regulated by cell geometry in a ligand-independent manner. These biophysical changes trigger signalling events involving the serine/threonine kinase Akt/protein kinase B (PKB) that direct cell-geometry-dependent mesenchymal stem cell differentiation. Our study defines a central regulatory role by plasma membrane ordered lipid raft microdomains in modulating stem cell differentiation with potential translational applications.
date: 2018-02-12
date_type: published
publisher: NATURE PUBLISHING GROUP
official_url: https://doi.org/10.1038/s41563-017-0014-0
oa_status: green
full_text_type: other
language: eng
primo: open
primo_central: open_green
article_type_text: Article
verified: verified_manual
elements_id: 1537883
doi: 10.1038/s41563-017-0014-0
lyricists_name: Bertazzo, Sergio
lyricists_id: SBERT93
actors_name: Bertazzo, Sergio
actors_id: SBERT93
actors_role: owner
full_text_status: public
publication: Nature Materials
volume: 17
number: 3
pagerange: 237-242
pages: 8
issn: 1476-4660
citation:        von Erlach, TC;    Bertazzo, S;    Wozniak, MA;    Horejs, C-M;    Maynard, SA;    Attwood, S;    Robinson, BK;                         ... Stevens, MM; + view all <#>        von Erlach, TC;  Bertazzo, S;  Wozniak, MA;  Horejs, C-M;  Maynard, SA;  Attwood, S;  Robinson, BK;  Autefage, H;  Kallepitis, C;  Hernandez, ADR;  Chen, CS;  Goldoni, S;  Stevens, MM;   - view fewer <#>    (2018)    Cell-geometry-dependent changes in plasma membrane order direct stem cell signalling and fate.                   Nature Materials , 17  (3)   pp. 237-242.    10.1038/s41563-017-0014-0 <https://doi.org/10.1038/s41563-017-0014-0>.       Green open access   
 
document_url: https://discovery.ucl.ac.uk/id/eprint/10045875/1/von%20erlach%20et%20al%20manuscript%20nature%20materials%20revised%20final.pdf