TY  - JOUR
SP  - 237
VL  - 17
IS  - 3
N1  - This version is the author accepted manuscript. For information on re-use, please refer to the publisher?s terms and conditions.
UR  - https://doi.org/10.1038/s41563-017-0014-0
SN  - 1476-4660
A1  - von Erlach, TC
A1  - Bertazzo, S
A1  - Wozniak, MA
A1  - Horejs, C-M
A1  - Maynard, SA
A1  - Attwood, S
A1  - Robinson, BK
A1  - Autefage, H
A1  - Kallepitis, C
A1  - Hernandez, ADR
A1  - Chen, CS
A1  - Goldoni, S
A1  - Stevens, MM
JF  - Nature Materials
AV  - public
Y1  - 2018/02/12/
EP  - 242
TI  - Cell-geometry-dependent changes in plasma membrane order direct stem cell signalling and fate
PB  - NATURE PUBLISHING GROUP
N2  - Cell size and shape affect cellular processes such as cell survival, growth and differentiation1,2,3,4, thus establishing cell geometry as a fundamental regulator of cell physiology. The contributions of the cytoskeleton, specifically actomyosin tension, to these effects have been described, but the exact biophysical mechanisms that translate changes in cell geometry to changes in cell behaviour remain mostly unresolved. Using a variety of innovative materials techniques, we demonstrate that the nanostructure and lipid assembly within the cell plasma membrane are regulated by cell geometry in a ligand-independent manner. These biophysical changes trigger signalling events involving the serine/threonine kinase Akt/protein kinase B (PKB) that direct cell-geometry-dependent mesenchymal stem cell differentiation. Our study defines a central regulatory role by plasma membrane ordered lipid raft microdomains in modulating stem cell differentiation with potential translational applications.
ID  - discovery10045875
KW  - Science & Technology
KW  -  Physical Sciences
KW  -  Technology
KW  -  Chemistry
KW  -  Physical
KW  -  Materials Science
KW  -  Multidisciplinary
KW  -  Physics
KW  -  Applied
KW  -  Physics
KW  -  Condensed Matter
KW  -  Chemistry
KW  -  Materials Science
KW  -  Physics
KW  -  LIPID RAFTS
KW  -  CAVEOLAE
KW  -  MICROTUBULES
KW  -  MICRODOMAINS
KW  -  GROWTH
KW  -  SWITCH
ER  -