@article{discovery10045518, month = {March}, journal = {Veterinary Ophthalmology}, pages = {206--212}, note = {This version is the author accepted manuscript. For information on re-use, please refer to the publisher's terms and conditions.}, year = {2019}, volume = {22}, number = {2}, title = {Immunohistochemical characterization of feline lymphoplasmacytic anterior uveitis}, abstract = {OBJECTIVE: To characterize the immune cells present in different forms of feline anterior uveitis. SAMPLES: Eyes were obtained from 49 cats diagnosed with chronic idiopathic lymphoplasmacytic anterior uveitis, 7 cats with feline infectious peritonitis (FIP), and 9 cats euthanized for nonocular disease. METHODS: H\&E sections were scored on the level of infiltrate in the anterior uvea. Immunohistochemistry was performed for FoxP3, CD3, and IL-17A, and positive cells were quantified in multiple images of each sample. A generalized estimating equation�tested for an association between the level of inflammation and the prevalence of these cell types. RESULTS: Cells stained positive for IL-17A in idiopathic uveitis but not in FIP samples. We found significantly fewer FoxP3+and CD3+cells in low-grade compared with high-grade inflammation in idiopathic uveitis or FIP samples (P values all {\ensuremath{<}}.005), but no difference between FIP and high-grade samples. CONCLUSIONS: Idiopathic, but not FIP-associated, uveitis appears to have Th17 cell involvement. The numbers of FoxP3+and CD3+T-cells present appear directly correlated; thus, the severity of disease does not appear directly determined by the numbers of regulatory cells.}, url = {https://doi.org/10.1111/vop.12569}, author = {Crossfield, L and Fortuna, L and Carling, R and Scurrell, E and Matas Riera, M and Chang, Y-M and Dawson, C and Pittaway, R and Yeung, IY and Thaung, C and Priestnall, SL and Garden, OA}, keywords = {CD3, FoxP3, IL17, feline, immunohistochemistry, uveitis}, issn = {1463-5224} }