eprintid: 10044565
rev_number: 20
eprint_status: archive
userid: 608
dir: disk0/10/04/45/65
datestamp: 2018-03-06 14:20:26
lastmod: 2021-12-13 23:56:31
status_changed: 2018-03-06 14:20:26
type: article
metadata_visibility: show
creators_name: Wang, Y
creators_name: Alhaque, S
creators_name: Cameron, K
creators_name: Meseguer-Ripolles, J
creators_name: Lucendo-Villarin, B
creators_name: Rashidi, H
creators_name: Hay, DC
title: Defined and Scalable Generation of Hepatocyte-like Cells from Human Pluripotent Stem Cells
ispublished: pub
divisions: UCL
divisions: B02
divisions: D13
divisions: G22
note: Copyright © 2017 Creative Commons Attribution 3.0 License
abstract: Human pluripotent stem cells (hPSCs) possess great value for biomedical research. hPSCs can be scaled and differentiated to all cell types
found in the human body. The differentiation of hPSCs to human hepatocyte-like cells (HLCs) has been extensively studied, and efficient
differentiation protocols have been established. The combination of extracellular matrix and biological stimuli, including growth factors, cytokines,
and small molecules, have made it possible to generate HLCs that resemble primary human hepatocytes. However, the majority of procedures
still employ undefined components, giving rise to batch-to-batch variation. This serves as a significant barrier to the application of the technology.
To tackle this issue, we developed a defined system for hepatocyte differentiation using human recombinant laminins as extracellular matrices in
combination with a serum-free differentiation process. Highly efficient hepatocyte specification was achieved, with demonstrated improvements in
both HLC function and phenotype. Importantly, this system is easy to scale up using research and GMP-grade hPSC lines promising advances in
cell-based modelling and therapies.
date: 2017-03-01
date_type: published
publisher: JOURNAL OF VISUALIZED EXPERIMENTS
official_url: http://doi.org/10.3791/55355
oa_status: green
full_text_type: pub
language: eng
primo: open
primo_central: open_green
verified: verified_manual
elements_id: 1539269
doi: 10.3791/55355
lyricists_name: Rashidi, Hassan
lyricists_id: HRASH80
actors_name: Waragoda Vitharana, Nimal
actors_id: NWARR44
actors_role: owner
full_text_status: public
publication: JOVE-Journal of Visualized Experiments
number: 121
pages: 8
issn: 1940-087X
citation:        Wang, Y;    Alhaque, S;    Cameron, K;    Meseguer-Ripolles, J;    Lucendo-Villarin, B;    Rashidi, H;    Hay, DC;      (2017)    Defined and Scalable Generation of Hepatocyte-like Cells from Human Pluripotent Stem Cells.                   JOVE-Journal of Visualized Experiments   (121)      10.3791/55355 <https://doi.org/10.3791/55355>.       Green open access   
 
document_url: https://discovery.ucl.ac.uk/id/eprint/10044565/1/jove-121-55355.pdf