TY  - JOUR
IS  - 121
N1  - Copyright © 2017 Creative Commons Attribution 3.0 License
TI  - Defined and Scalable Generation of Hepatocyte-like Cells from Human Pluripotent Stem Cells
AV  - public
Y1  - 2017/03/01/
EP  - 8
JF  - JOVE-Journal of Visualized Experiments
A1  - Wang, Y
A1  - Alhaque, S
A1  - Cameron, K
A1  - Meseguer-Ripolles, J
A1  - Lucendo-Villarin, B
A1  - Rashidi, H
A1  - Hay, DC
N2  - Human pluripotent stem cells (hPSCs) possess great value for biomedical research. hPSCs can be scaled and differentiated to all cell types
found in the human body. The differentiation of hPSCs to human hepatocyte-like cells (HLCs) has been extensively studied, and efficient
differentiation protocols have been established. The combination of extracellular matrix and biological stimuli, including growth factors, cytokines,
and small molecules, have made it possible to generate HLCs that resemble primary human hepatocytes. However, the majority of procedures
still employ undefined components, giving rise to batch-to-batch variation. This serves as a significant barrier to the application of the technology.
To tackle this issue, we developed a defined system for hepatocyte differentiation using human recombinant laminins as extracellular matrices in
combination with a serum-free differentiation process. Highly efficient hepatocyte specification was achieved, with demonstrated improvements in
both HLC function and phenotype. Importantly, this system is easy to scale up using research and GMP-grade hPSC lines promising advances in
cell-based modelling and therapies.
ID  - discovery10044565
UR  - http://doi.org/10.3791/55355
PB  - JOURNAL OF VISUALIZED EXPERIMENTS
SN  - 1940-087X
ER  -