eprintid: 10043459 rev_number: 27 eprint_status: archive userid: 608 dir: disk0/10/04/34/59 datestamp: 2018-02-15 11:08:39 lastmod: 2021-10-03 23:52:20 status_changed: 2018-02-15 11:08:39 type: article metadata_visibility: show creators_name: Rusbridge, C creators_name: Salguero, FJ creators_name: David, MA creators_name: Faller, KME creators_name: Bras, JT creators_name: Guerreiro, RJ creators_name: Richard-Londt, AC creators_name: Grainger, D creators_name: Head, E creators_name: Brandner, SGP creators_name: Summers, B creators_name: Hardy, J creators_name: Tayebi, M title: An Aged Canid with Behavioral Deficits Exhibits Blood and Cerebrospinal Fluid Amyloid Beta Oligomers ispublished: pub divisions: UCL divisions: B02 divisions: C07 divisions: D07 divisions: F86 keywords: Science & Technology, Life Sciences & Biomedicine, Geriatrics & Gerontology, Neurosciences, Neurosciences & Neurology, canine cognitive dysfunction, Alzheimer, canine, A beta oligomers, neuropathology, aggregation, neurotoxicity, ALZHEIMERS-DISEASE, BRAIN, DOGS, ANTIBODIES, PROTEINS, PATHWAY, MODEL note: © 2018 Rusbridge, Salguero, David, Faller, Bras, Guerreiro, Richard-Londt, Grainger, Head, Brandner, Summers, Hardy and Tayebi. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. abstract: Many of the molecular and pathological features associated with human Alzheimer disease (AD) are mirrored in the naturally occurring age-associated neuropathology in the canine species. In aged dogs with declining learned behavior and memory the severity of cognitive dysfunction parallels the progressive build up and location of Aβ in the brain. The main aim of this work was to study the biological behavior of soluble oligomers isolated from an aged dog with cognitive dysfunction through investigating their interaction with a human cell line and synthetic Aβ peptides. We report that soluble oligomers were specifically detected in the dog's blood and cerebrospinal fluid (CSF) via anti-oligomer- and anti-Aβ specific binders. Importantly, our results reveal the potent neurotoxic effects of the dog's CSF on cell viability and the seeding efficiency of the CSF-borne soluble oligomers on the thermodynamic activity and the aggregation kinetics of synthetic human Aβ. The value of further characterizing the naturally occurring Alzheimer-like neuropathology in dogs using genetic and molecular tools is discussed. date: 2018-01-30 date_type: published publisher: FRONTIERS MEDIA SA official_url: http://dx.doi.org/10.3389/fnagi.2018.00007 oa_status: green full_text_type: pub language: eng primo: open primo_central: open_green article_type_text: Article verified: verified_manual elements_id: 1534205 doi: 10.3389/fnagi.2018.00007 language_elements: English lyricists_name: Brandner, Sebastian lyricists_name: Hardy, John lyricists_name: Louro Guerreiro, Rita lyricists_name: Smalley, June lyricists_name: Tomas Bras, Jose lyricists_id: SBRAN30 lyricists_id: JHARD28 lyricists_id: RJLOU51 lyricists_id: JASMA87 lyricists_id: JMTOM86 actors_name: Bracey, Alan actors_id: ABBRA90 actors_role: owner full_text_status: public publication: Frontiers in Aging Neuroscience volume: 10 article_number: 7 pages: 8 issn: 1663-4365 citation: Rusbridge, C; Salguero, FJ; David, MA; Faller, KME; Bras, JT; Guerreiro, RJ; Richard-Londt, AC; ... Tayebi, M; + view all <#> Rusbridge, C; Salguero, FJ; David, MA; Faller, KME; Bras, JT; Guerreiro, RJ; Richard-Londt, AC; Grainger, D; Head, E; Brandner, SGP; Summers, B; Hardy, J; Tayebi, M; - view fewer <#> (2018) An Aged Canid with Behavioral Deficits Exhibits Blood and Cerebrospinal Fluid Amyloid Beta Oligomers. Frontiers in Aging Neuroscience , 10 , Article 7. 10.3389/fnagi.2018.00007 <https://doi.org/10.3389/fnagi.2018.00007>. Green open access document_url: https://discovery.ucl.ac.uk/id/eprint/10043459/1/fnagi-10-00007.pdf