eprintid: 10042361 rev_number: 25 eprint_status: archive userid: 608 dir: disk0/10/04/23/61 datestamp: 2018-02-02 10:51:06 lastmod: 2022-01-24 01:57:44 status_changed: 2018-02-02 10:51:06 type: article metadata_visibility: show creators_name: Saracino, A creators_name: Cozzi-Lepri, A creators_name: Shanyinde, M creators_name: Ceccherini Silberstein, F creators_name: Nozza, S creators_name: Di Biagio, A creators_name: Cassola, G creators_name: Bruno, G creators_name: Capobianchi, M creators_name: Puoti, M creators_name: Monno, L creators_name: d'Arminio Monforte, A creators_name: ICONA Foundation Study, title: HIV-1 co-receptor tropism and liver fibrosis in HIV-infected patients ispublished: pub divisions: UCL divisions: B02 divisions: D01 divisions: G16 note: : © 2018 Saracino et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. abstract: BACKGROUND: In vitro, gp120 of both X4 and R5 HIV-1 strains activates human hepatic stellate cells, but if it can promote liver fibrosis in vivo is unknown. We aimed to evaluate if patients carrying X4 or R5 strains have a different liver fibrosis (LF) progression over time. METHODS: A total of 1,137 HIV-infected patients in ICONA cohort (21% females, 7% HCV co-infected) with an available determination of HIV-1 co-receptor tropism (CRT), a Fibrosis-4 Index for Liver Fibrosis (FIB-4) <3.25 and at least one-year follow-up were included. CRT was assessed by gp120 sequencing on plasma RNA and geno2pheno algorithm (10% false positive rate) or by Trofile. LF was assessed by means of FIB-4. LF progression was defined as an absolute score increase or a transition to higher fibrosis stratum and/or occurrence of liver-related clinical events. RESULTS: A total of 249 (22%) patients carried X4 strains, which were associated with older age, lower CD4 count, lower nadir CD4, and intravenous drug use. Overall, X4 and R5 patients had similar baseline FIB-4 scores and similar mean FIB-4 slope after a median follow-up of 35 months. There was no difference between X4 and R5 for time to LF progression (p = 0.925). Estimated risk of LF at 24 months (95% CI) after baseline in X4 and R5 was 10.6% (8.3-12.9) and 9.9% (5.9-14.0), respectively. Age, HCV co-infection, diabetes, HIV-duration, HIV-RNA>100.000 cp/mL, antiretroviral therapy exposure were associated with LF progression at multivariate analysis. CONCLUSIONS: A slight LF progression over time was observed in HIV-infected patients. No difference was demonstrated for X4 and R5 HIV-1 strains in accelerating LF evolution. date: 2018 date_type: published official_url: http://dx.doi.org/10.1371/journal.pone.0190302 oa_status: green full_text_type: pub pmcid: PMC5764264 language: eng primo: open primo_central: open_green article_type_text: Journal Article verified: verified_manual elements_id: 1528132 doi: 10.1371/journal.pone.0190302 pii: PONE-D-17-29533 lyricists_name: Cozzi-Lepri, Alessandro lyricists_id: ACOZZ70 actors_name: Laslett, David actors_id: DLASL34 actors_role: owner full_text_status: public publication: PLoS One volume: 13 number: 1 article_number: e0190302 event_location: United States issn: 1932-6203 citation: Saracino, A; Cozzi-Lepri, A; Shanyinde, M; Ceccherini Silberstein, F; Nozza, S; Di Biagio, A; Cassola, G; ... ICONA Foundation Study; + view all <#> Saracino, A; Cozzi-Lepri, A; Shanyinde, M; Ceccherini Silberstein, F; Nozza, S; Di Biagio, A; Cassola, G; Bruno, G; Capobianchi, M; Puoti, M; Monno, L; d'Arminio Monforte, A; ICONA Foundation Study; - view fewer <#> (2018) HIV-1 co-receptor tropism and liver fibrosis in HIV-infected patients. PLoS One , 13 (1) , Article e0190302. 10.1371/journal.pone.0190302 <https://doi.org/10.1371/journal.pone.0190302>. Green open access document_url: https://discovery.ucl.ac.uk/id/eprint/10042361/1/journal.pone.0190302.pdf