eprintid: 10037991
rev_number: 20
eprint_status: archive
userid: 608
dir: disk0/10/03/79/91
datestamp: 2017-11-21 14:48:57
lastmod: 2021-10-15 23:10:03
status_changed: 2017-11-21 14:48:57
type: article
metadata_visibility: show
creators_name: Khorsandi, SE
creators_name: Taanman, JW
creators_name: Heaton, N
title: Subunit composition of respiratory chain complex 1 and its responses to oxygen in mitochondria from human donor livers
ispublished: pub
divisions: UCL
divisions: B02
divisions: C07
divisions: D07
divisions: F84
keywords: Complex 1, Donor after cardiac death, Liver transplantation, Mitochondria, Oxygen
note: Copyright © The Author(s) 2017. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License
(http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium,
provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license,
and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/
publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
abstract: OBJECTIVE: Donor liver function in transplantation is defined by mitochondrial function and the ability of mitochondria to recover from the sequence of warm and/or cold ischemia. Mitochondrial resilience maybe related to assembly and- subunit composition of Complex 1. The aim of this study was to determine if Complex 1 subunit composition was different in donor livers of varying quality and whether oxygen exposure had any effect. RESULTS: Five human livers not suitable for transplant were split. One half placed in cold static storage and the other half exposed to 40% oxygen for 2 h. Protein was extracted for western blot. Membranes were probed with antibodies against β-actin and the following subunits of Complex 1: MTND1, NDUFA10, NDUFB6 and NDUFV2. No difference in steady state Complex 1 subunit composition was demonstrated between donor livers of varying quality, in terms of steatosis or mode of donation. Neither did exposure to oxygen influence Complex 1 subunit composition. This small observational study on subunit levels suggest that Complex 1 is fully assembled as no degradation of subunits associated with the different parts of the enzyme was seen.
date: 2017-11-02
date_type: published
official_url: http://doi.org/10.1186/s13104-017-2863-7
oa_status: green
full_text_type: pub
pmcid: PMC5667463
language: eng
primo: open
primo_central: open_green
verified: verified_manual
elements_id: 1509664
doi: 10.1186/s13104-017-2863-7
pii: 10.1186/s13104-017-2863-7
lyricists_name: Taanman, Jan
lyricists_id: JWTAA74
actors_name: Taanman, Jan
actors_id: JWTAA74
actors_role: owner
full_text_status: public
publication: BMC Research Notes
volume: 10
article_number: 547
event_location: England
issn: 1756-0500
citation:        Khorsandi, SE;    Taanman, JW;    Heaton, N;      (2017)    Subunit composition of respiratory chain complex 1 and its responses to oxygen in mitochondria from human donor livers.                   BMC Research Notes , 10     , Article 547.  10.1186/s13104-017-2863-7 <https://doi.org/10.1186/s13104-017-2863-7>.       Green open access   
 
document_url: https://discovery.ucl.ac.uk/id/eprint/10037991/1/Subunit%20composition%20of%20respiratory%20chain%20complex%201%20and%20its%20responses%20to%20oxygen%20in%20mitochondria%20from%20human%20donor%20livers.pdf