eprintid: 10025976
rev_number: 26
eprint_status: archive
userid: 608
dir: disk0/10/02/59/76
datestamp: 2017-10-26 13:31:36
lastmod: 2021-11-07 00:20:18
status_changed: 2017-10-26 13:31:36
type: article
metadata_visibility: show
creators_name: McLeod, F
creators_name: Marzo, A
creators_name: Podpolny, M
creators_name: Galli, S
creators_name: Salinas, P
title: Evaluation of Synapse Density in Hippocampal Rodent Brain Slices.
ispublished: pub
divisions: UCL
divisions: B02
divisions: C08
divisions: D09
divisions: F96
keywords: Neuroscience, Issue 128, Neurons, synapses, brain slices, immunofluorescence, hippocampus, mouse, rat, vGlut1, PSD-95, vGAT,
gephyrin
note: Copyright © 2017 Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported
License
abstract: In the brain, synapses are specialized junctions between neurons, determining the strength and spread of neuronal signaling. The number of synapses is tightly regulated during development and neuronal maturation. Importantly, deficits in synapse number can lead to cognitive dysfunction. Therefore, the evaluation of synapse number is an integral part of neurobiology. However, as synapses are small and highly compact in the intact brain, the assessment of absolute number is challenging. This protocol describes a method to easily identify and evaluate synapses in hippocampal rodent slices using immunofluorescence microscopy. It includes a three-step procedure to evaluate synapses in high-quality confocal microscopy images by analyzing the co-localization of pre- and postsynaptic proteins in hippocampal slices. It also explains how the analysis is performed and gives representative examples from both excitatory and inhibitory synapses. This protocol provides a solid foundation for the analysis of synapses and can be applied to any research investigating the structure and function of the brain.
date: 2017-10
date_type: published
official_url: http://dx.doi.org/10.3791/56153
oa_status: green
full_text_type: pub
language: eng
primo: open
primo_central: open_green
article_type_text: Journal Article
verified: verified_manual
elements_id: 1465261
doi: 10.3791/56153
language_elements: eng
lyricists_name: Marzo, Aude
lyricists_name: McLeod, Faye
lyricists_name: Podpolny, Marina
lyricists_name: Salinas, Patricia
lyricists_id: AMARZ78
lyricists_id: FMCLE94
lyricists_id: MTETE37
lyricists_id: PSALI67
actors_name: Cuccu, Clara
actors_id: CCCUC40
actors_role: owner
full_text_status: public
publication: J Vis Exp
number: 128
article_number: e56153
event_location: United States
issn: 1940-087X
citation:        McLeod, F;    Marzo, A;    Podpolny, M;    Galli, S;    Salinas, P;      (2017)    Evaluation of Synapse Density in Hippocampal Rodent Brain Slices.                   J Vis Exp   (128)    , Article e56153.  10.3791/56153 <https://doi.org/10.3791/56153>.       Green open access   
 
document_url: https://discovery.ucl.ac.uk/id/eprint/10025976/1/Evaluation%20of%20Synapse%20Densit.pdf