eprintid: 10024857
rev_number: 25
eprint_status: archive
userid: 608
dir: disk0/10/02/48/57
datestamp: 2017-10-17 13:13:55
lastmod: 2021-09-20 22:26:14
status_changed: 2017-10-17 13:13:55
type: article
metadata_visibility: show
creators_name: Williams, GR
title: Insulin-loaded PLGA microspheres for glucose-responsive release
ispublished: pub
divisions: UCL
divisions: B02
divisions: C08
divisions: D10
divisions: G08
keywords: Porous microspheres, layer-by-layer, glucose sensitive, sustained release, smart drug delivery system
note: © 2017 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use,
distribution, and reproduction in any medium, provided the original work is properly cited.
abstract: Porous poly(lactic-co-glycolic acid) (PLGA) microspheres were prepared, loaded with insulin, and then coated in poly(vinyl alcohol) (PVA) and a novel boronic acid-containing copolymer [poly(acrylamide phenyl boronic acid-co-N–vinylcaprolactam); p(AAPBA-co-NVCL)]. Multilayer microspheres were generated using a layer-by-layer approach depositing alternating coats of PVA and p(AAPBA-co-NVCL) on the PLGA surface, with the optimal system found to be that with eight alternating layers of each coating. The resultant material comprised spherical particles with a porous PLGA core and the pores covered in the coating layers. Insulin could successfully be loaded into the particles, with loading capacity and encapsulation efficiencies reaching 2.83 ± 0.15 and 82.6 ± 5.1% respectively, and was found to be present in the amorphous form. The insulin-loaded microspheres could regulate drug release in response to a changing concentration of glucose. In vitro and in vivo toxicology tests demonstrated that they are safe and have high biocompatibility. Using the multilayer microspheres to treat diabetic mice, we found they can effectively control blood sugar levels over at least 18 days, retaining their glucose-sensitive properties during this time. Therefore, the novel multilayer microspheres developed in this work have significant potential as smart drug-delivery systems for the treatment of diabetes.
date: 2017-10-04
date_type: published
publisher: Informa Healthcare
official_url: http://dx.doi.org/10.1080/10717544.2017.1381200
oa_status: green
full_text_type: pub
language: eng
primo: open
primo_central: open_green
article_type_text: Article
verified: verified_manual
elements_id: 1441227
doi: 10.1080/10717544.2017.1381200
lyricists_name: Williams, Gareth
lyricists_id: GWILL02
actors_name: Williams, Gareth
actors_id: GWILL02
actors_role: owner
full_text_status: public
publication: Drug Delivery
volume: 24
number: 1
pagerange: 1513-1525
issn: 1071-7544
citation:        Williams, GR;      (2017)    Insulin-loaded PLGA microspheres for glucose-responsive release.                   Drug Delivery , 24  (1)   pp. 1513-1525.    10.1080/10717544.2017.1381200 <https://doi.org/10.1080/10717544.2017.1381200>.       Green open access   
 
document_url: https://discovery.ucl.ac.uk/id/eprint/10024857/7/Williams_Insulin%20loaded%20PLGA%20microspheres%20for%20glucose%20responsive%20release.pdf