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[(an)19.7(d)-243(p)0(r)15.1(e)0(s)19.9(e)0(r)19.7(v)0(a)19.7(t)0(i)20.6(o)0(n)-232.6(o)0(f)-238.3(t)0(h)20.7(e)-244.2(ar)19.7(ch)19.7(it)20.6(ec)12.8(tu)20.7(re)-233.7(of)-232.6(En)19.6(gN)20(T.)-236.1(St)20.9(or)15.1(ag)19.7(e)-244.2(m)0(e)18.4(d)0(i)20.7(a)]TJ
0 -1.1116 TD
[(an)19.7(d)-392.8(p)0(r)20.9(o)0(t)14.9(o)0(c)19.7(o)0(l)20.7(s)-392.6(we)18.8(re)-383.5(se)14.1(le)19.6(ct)19.5(ed)-383.5(an)19.7(d)-392.8(c)0(o)19.7(m)0(p)19.6(a)0(r)14(e)0(d)-383.5(u)0(s)21(i)0(n)20.7(g)-392.8(si)20.9(mp)19.6(li)14.8(e)19.8(d)]TJ
T*
[(ti)20.6(ss)15.5(ue)-441.1(co)19.7(ns)15.3(tr)20.7(uc)19.7(ts)-445.6(to)-440.1(in)15(ve)19.7(st)20.9(ig)20.7(at)19.5(e)-457.3(c)0(e)18.5(l)0(l)-446(v)0(i)20.7(a)0(b)19.7(i)0(l)20.6(i)0(t)20.6(y)-456.1(af)19.7(te)19.6(r)-456.1(d)0(i)20.7(f)0(f)20.9(e)0(r)14(e)0(n)19.7(t)]TJ
0 -1.1174 TD
[(st)20.9(or)15.1(ag)19.7(e)-319(p)0(e)19.7(r)0(i)15(o)0(d)20.8(s)0(,)-309.6(t)0(h)15(e)0(n)-308.6(t)0(h)20.7(e)-324.8(ab)19.7(il)20.6(it)14.8(y)-317.9(o)0(f)-313.2(p)0(r)20.9(o)0(m)19.6(i)0(s)20.9(i)0(n)15(g)-317.9(ca)18.5(nd)15.1(id)20.7(at)19.5(e)-319(c)0(o)13.9(n)0(-)]TJ
0 -1.1116 TD
[(di)20.7(ti)20.6(on)15.1(s)-173.7(t)0(o)-163.6(p)0(r)20.9(e)0(s)14.1(e)0(r)19.7(v)0(e)-164.6(E)0(n)19.6(g)0(N)20(T)-175.1(st)20.9(ru)15.1(ct)19.5(ur)20.9(e)-175(a)0(n)19.7(d)-173.9(fu)20.9(nc)13.9(ti)20.6(on)-163.5(wa)18.8(s)-173.7(a)0(s)19.9(s)0(e)14.1(s)0(s)21.2(e)0(d)19.7(.)]TJ
/F7 1 Tf
8.9663 0 0 8.9663 62.022 319.5779 Tm
[(Materials)-332.7(and)-338.2(Methods)]TJ
/F10 1 Tf
0 -1.8336 TD
[(Production)-338.3(of)-330.4(cellular)-334.6(collagen)-338(gels)]TJ
/F4 1 Tf
9.843 0 0 9.843 72 286.696 Tm
[(Col)12.8(la)13.8(gen)-269.4(hyd)14(rog)14(el)13.8(s)-277.4(con)12.8(tai)12.6(nin)13.8(g)-271.8(Sch)13(wan)11.9(n)-271.8(cel)11.5(ls)-272.8(wer)12(e)-273(man)11.6(u-)]TJ
-1.0137 -1.1116 TD
[(fac)11.7(tur)13.9(ed)-429.6(acc)10.5(ord)14(ing)-423.9(to)-434.3(pre)12.8(vio)13.8(usl)14(y)-433.1(est)12.9(abl)12.7(ish)14(ed)-435.3(pro)19.8(toc)12.7(ols)-429.5(and)]TJ
T*
[(sta)12.9(bil)13.7(ize)11.5(d)-352.4(usi)14(ng)-347.8(pla)12.7(sti)13.9(c)-359.4(c)0(o)13.9(mpr)12.8(ess)13.2(ion)13.9(.)]TJ
7.3823 0 0 6.5614 208.3464 269.1779 Tm
(20)Tj
9.843 0 0 9.843 219.2314 264.8125 Tm
[(Sch)13(wa)13(nn)-353.5(cel)17.3(ls)-353.5(\(SC)13.1(L)]TJ
-15.9717 -1.1174 TD
[(4.1)17.4(/F7)14(;)-450.5(H)0(e)13(alt)12.6(h)-444.6(Pro)14.2(tec)11.5(tio)13.7(n)-444.6(Age)11.9(ncy)12.8(,)-441.2(Uni)13(ted)-436.6(Kin)13(gdo)14(m\))-441.1(wer)12(e)]TJ
0 -1.1116 TD
[(mai)11.5(nta)12.7(in)15(ed)-210.7(in)-209.7(DME)11(M)-215.1(\(Gi)13(bco)]TJ
/F5 1 Tf
7.3823 0 0 6.5614 177.0519 247.2377 Tm
()Tj
/F4 1 Tf
9.843 0 0 9.843 182.8346 242.8724 Tm
[(\))-214.2(sup)14.2(ple)12.7(men)11.6(te)13.8(d)-214.2(wit)12.8(h)-214.2(pen)12.8(ici)12.6(lli)13.6(n)]TJ
-12.2739 -1.1116 TD
[(and)-384.6(str)14.1(ept)12.7(om)13.9(yci)12.7(n)-392.8(\(10)14(0)-162.4(U/m)17.5(L)-394(and)-384.6(100)-153.1(mg)13.9(/mL)11.4(,)-389.3(res)13(pec)11.7(tiv)13.7(ely)12.7(;)]TJ
0 -1.1174 TD
[(Sig)14(ma,)-399.7(Uni)13(ted)-402(Kin)13(gdo)14(m\))-406.6(an)13.9(d)-415.8(1)0(0)15.1(%)-411.1(v/v)-400.8(fet)12.7(al)-406.7(bov)14(ine)-402(ser)13(um)]TJ
0 -1.1116 TD
[(\(FB)13.1(S;)-319(Gib)13(co\))-321.2(in)-319.1(sta)12.9(nda)12.8(rd)-318.9(cel)11.5(l)-323.8(cul)12.7(tur)13.9(e)-330.6()0(a)14.1(sks)-319.7(in)-319.1(a)-330.6(3)0(7)]TJ
/F5 1 Tf
20.8674 0 TD
()Tj
/F4 1 Tf
.3917 0 TD
[(C)-324.7(inc)12.7(u-)]TJ
-21.2591 -1.1116 TD
[(bat)12.7(or)-532.1(wi)14.1(th)-532.2(5%)-533.2(ca)12.8(rbo)14(n)-536.7(dio)13.9(xid)13.8(e)-532.2(\(CO)]TJ
7.3823 0 0 6.5614 211.6346 197.4047 Tm
(2)Tj
9.843 0 0 9.843 215.2629 199.0488 Tm
[(\))-536.7(and)-528.6(pa)13.9(ssa)13.2(ged)-528.6(wit)12.8(h)]TJ
-15.5685 -1.1174 TD
[(try)13.9(psi)14(n/E)12.7(DT)13(A)-422.4(\(Li)12.7(fe)-412.3(Tec)10.5(hno)14(log)13.8(ie)13.8(s,)-419(Uni)13(te)13.8(d)-421.6(Kin)13(gdo)14(m\))-412.3(whe)11.9(n)]TJ
0 -1.1116 TD
[(req)12.8(uir)13.9(ed.)-289(Aci)11.8(d-s)14.2(olu)13.9(bil)13.7(iz)13.8(ed)-297.1(typ)13.8(e)-301.8(I)-300.6(bov)14(in)15(e)-301.8(col)12.7(lag)12.7(en)-297.1(\(3)-151.9(mg/)12.6(mL;)]TJ
T*
[(Kok)13.1(en,)-179.6(Jap)13(an\))-177.2(was)-183.7(dil)13.7(ute)12.7(d)-185.4(wit)12.8(h)-191.2(26.)17.4(8%)-187.6(v/v)-176.2(1)-162.4(m)0(M)-182.9(hyd)14(roc)12.9(hlo)13.8(ric)]TJ
T*
[(aci)11.5(d)-346.7(t)0(o)-336.4(a)-347.8(con)12.8(cen)11.7(tr)15(ati)12.6(on)-342(of)-342(2)-162.4(m)0(g)13.9(/mL)11.4(.)-343.2(Thi)12.6(s)-346.5(sol)14(uti)13.7(on)-336.3(was)-339.2(the)12.7(n)]TJ
0 -1.1174 TD
[(mix)12.6(ed)-181.9(wit)12.8(h)-185.4(10%)-177.2(v/v)-176.2(10)]TJ
/F5 1 Tf
9.2558 0 TD
()Tj
/F4 1 Tf
.7142 0 TD
[(min)12.6(imu)18.4(m)-192.4(ess)13.2(en)13.9(tia)12.6(l)-191.3(m)0(e)12.7(diu)13.8(m)-186.6(\(Si)14(gma)11.6(\))]TJ
-9.9701 -1.1116 TD
[(and)-499.8(neu)12.8(tra)12.7(liz)12.6(ed)-504.5(\()]TJ
/F11 1 Tf
7.2054 0 TD
(*)Tj
/F4 1 Tf
.9964 0 TD
[(0.)12.7(5%)-510.1(v/v)-498.8(neu)12.8(tra)12.7(liz)12.6(ing)-498.8(sol)14(uti)13.7(on;)-498.8(Lon)12.8(za)]TJ
-8.2018 -1.1116 TD
[(Bio)12.8(sci)12.9(enc)17.4(e,)-455(Uni)13(te)13.8(d)-456.1(Kin)13(gdo)14(m\))-452.7(be)13.9(for)14(e)-457.3(add)12.8(iti)13.6(on)-445.7(of)-451.4(10%)-447.9(v/v)]TJ
0 -1.1174 TD
[(cul)12.7(tur)13.9(e)-319(med)11.6(ia)-314.5(co)13.9(nta)12.7(ini)13.7(ng)-313.2(Sch)13(wan)11.9(n)-317.9(cel)11.5(ls.)-305.1(To)-314.4(pro)14(duc)12.8(e)-319(sim)12.8(ple)]TJ
0 -1.1116 TD
[(una)12.8(lig)13.7(ned)-338.5(gel)18.4(s)-352.2(for)-337.3(ini)13.7(tia)18.3(l)-352.6(e)0(x)13.9(per)12.8(ime)11.5(nts)14(,)-343.3(1)-162.4(mL)-344.4(of)-342(cel)11.5(lul)13.7(ar)-343.2(col)12.7(-)]TJ
T*
[(lag)12.7(en)-164.6(sol)14(ut)15(ion)-158.9(\(2)]TJ
/F5 1 Tf
6.6755 0 TD
()Tj
/F4 1 Tf
.7142 0 TD
(10)Tj
7.3823 0 0 6.5614 144.5102 93.8268 Tm
(6)Tj
9.843 0 0 9.843 149.8393 89.4614 Tm
[(cel)11.5(ls/)13.9(mL\))-155.4(was)-160.7(cas)11.8(t)-168.3(per)-159.9(wel)11.8(l)-162.5(o)0(f)-163.4(a)-169.3(24-)14(wel)11.8(l)]TJ
-8.9218 -1.1174 TD
[(pla)12.7(te.)-398.6(To)-406.6(man)11.6(ufa)12.8(ctu)12.7(re)-406.5(al)13.8(ign)13.8(ed)-406.5(gel)12.7(s)-409.8(for)-400.7(Eng)12.8(NT)-407.4(pro)14(du)15.1(cti)12.6(on,)]TJ
0 -1.1116 TD
(400)Tj
/F11 1 Tf
1.653 0 TD
(m)Tj
/F4 1 Tf
.5472 0 TD
[(L)-382.4(o)0(f)-376.5(cel)11.5(lul)13.7(ar)-377.7(col)18.4(lag)12.7(en)-377.7(sol)14(uti)13.7(on)-376.6(\(4)]TJ
/F5 1 Tf
13.8924 0 TD
()Tj
/F4 1 Tf
.7142 0 TD
(10)Tj
7.3823 0 0 6.5614 237.2031 71.8866 Tm
(6)Tj
9.843 0 0 9.843 244.6298 67.5212 Tm
[(cel)11.5(ls/)13.9(mL\))-374.3(was)]TJ
-18.552 -1.1116 TD
[(add)12.8(ed)-320.1(to)-319.1(tet)12.6(her)12.8(ing)-314.5(mo)13.8(lds)-320(th)15(at)-326(al)13.8(low)13(ed)-320.1(sel)12.9(f-a)12.9(lig)13.7(nme)11.6(nt)14.9(.)]TJ
7.3823 0 0 6.5614 275.4141 60.9449 Tm
(21)Tj
9.843 0 0 9.843 285.959 56.5795 Tm
(Un-)Tj
2.7531 67.9242 TD
[(ali)12.6(gne)12.8(d)-271.8(c)0(e)12.8(llu)13.7(lar)-263.7(co)13.9(lla)12.6(gen)-263.7(ge)13.9(ls)-267.1(wer)12(e)-267.2(use)13(d)-266(for)-262.4(the)-258(ini)13.7(tia)12.6(l)-266.2(exp)12.8(er-)]TJ
0 -1.1174 TD
[(ime)11.5(nts)-279.7(bec)17.4(aus)13(e)-290.2(a)-284.5(hig)13.9(her)-280.9(thr)13.9(oug)19.7(hpu)14(t)-289.2(cou)12.8(ld)-278.8(be)-285.6(ach)17.4(iev)12.7(ed)-285.6(and)-275.2(a)]TJ
0 -1.1116 TD
[(gre)12.9(ate)11.5(r)-404.2(num)12.8(ber)-396.1(of)-399.6(ide)12.7(nti)13.7(cal)-397.4(pie)12.7(ce)12.8(s)-409.8(cou)18.6(ld)-405.5(be)-400.8(obt)13.8(ai)13.8(ned)-396.1(fro)14(m)]TJ
T*
[(sim)12.8(ple)-292.6(una)12.8(lig)13.7(ned)-292.4(ge)13.9(ls)-295.9(com)11.6(par)12.8(ed)-297.1(wit)12.8(h)-300.6(E)0(n)13.9(gNT)11.9(.)-302.9(G)0(e)13(l)0(s)-295.9(wer)12(e)-301.8(in-)]TJ
T*
[(cub)12.8(ate)11.5(d)-369.7(a)0(t)-366.4(3)0(7)]TJ
/F5 1 Tf
5.5524 0 TD
()Tj
/F4 1 Tf
.3917 0 TD
[(C)-370.7(f)0(o)15.1(r)-369.7(15)-157.7(min)-361.8(to)-365.2(set)12.9(.)-366.3(Una)11.9(lig)13.7(ned)-361.6(col)12.7(la)13.8(gen)-361.6(gel)12.7(s)]TJ
-5.944 -1.1174 TD
[(wer)12(e)-209.6(sta)12.9(bil)13.7(ize)11.5(d)-208.4(for)-199.1(15)-157.7(mi)13.7(n)-208.4(usi)14(ng)-203.8(the)-200.4(RAF)12.2(T)]TJ
/F5 1 Tf
16.7607 0 TD
()Tj
/F4 1 Tf
1.0943 0 TD
[(abs)13(orb)14(er)-204.9(\(L)13.9(onz)12.8(a)]TJ
-17.8551 -1.1116 TD
[(Bio)12.8(sci)12.9(enc)11.7(e\).)-329.3(Tet)11.5(her)12.8(ed)-337.4(gel)12.7(s)-340.7(wer)12(e)-342.1(i)0(m)13.7(mer)11.6(sed)-332.6(in)-336.4(cul)12.7(tur)13.9(e)-342.1(m)0(e)12.7(dia)]TJ
T*
[(and)-263.7(in)15(cub)12.8(ate)11.5(d)-266(for)-256.7(24)-157.7(h)-266(t)0(o)-261.5(all)12.6(ow)-262.3(cel)11.5(lul)13.7(ar)-262.5(sel)12.9(f-a)12.9(lig)13.7(nm)13.9(ent)12.7(,)]TJ
7.3823 0 0 6.5614 529.0015 652.8187 Tm
(1)Tj
9.843 0 0 9.843 535.2943 648.4534 Tm
[(the)12.7(n)]TJ
-22.578 -1.1174 TD
[(med)11.6(ia)-303(rem)11.6(ove)12.8(d)-306.4(b)0(e)13.9(for)14(e)-307.5(sta)12.9(bil)13.7(iza)11.5(tio)13.7(n)-306.4(usi)14(ng)-301.7(RAF)12.2(T)-307.6(t)0(o)-301.8(gen)18.6(era)11.7(te)]TJ
0 -1.1116 TD
[(Eng)12.8(NT.)]TJ
/F10 1 Tf
8.9663 0 0 8.9663 313.0582 604.5731 Tm
[(Preservation)-339.9(conditions)]TJ
/F4 1 Tf
9.843 0 0 9.843 323.0361 588.1321 Tm
[(Unaligned)-364.7(cellular)-358.2(collagen)-363.9(gels)-356.8(were)-364.7(used)-356.7(in)-359.4(initial)-355.1(ex-)]TJ
-1.0137 -1.1174 TD
[(periments)-376.4(to)-365.2(determine)-372(the)-367.5(appropriate)-372.9(preservation)-373.8(condi-)]TJ
0 -1.1116 TD
[(tions)-253.2(for)-250.9(cell)-254.7(survival.)-255.4(Each)-249.8(circular)-260.2(gel)-252.3(was)-252.8(cut)-252.3(radially)-253.4(into)]TJ
T*
[(si)15.1(x)-289.1(e)0(q)19.7(u)0(a)19.7(l)-295(se)19.9(ct)19.5(or)15.1(s)-288.9(t)0(h)20.7(a)0(t)-285.7(w)0(e)18.8(r)0(e)-279.8(t)0(r)15(a)0(n)19.7(s)0(f)21.1(e)0(r)19.7(r)0(e)14(d)-289.1(to)-278.8(si)15.1(x)-289.1(d)0(i)20.7(f)0(f)15.1(e)0(r)19.7(e)0(n)19.7(t)-289.2(pr)15.1(es)19.9(er)19.7(-)]TJ
T*
[(va)13.9(ti)20.6(on)-295.9(co)13.9(nd)20.8(it)20.6(io)20.7(ns)-301.5(fo)20.9(r)-312.1(2)-306.4(da)19.7(ys)21(.)-308.7(T)0(h)13.9(r)0(e)19.7(e)-307.5(pi)20.7(ec)18.5(es)-302.7(we)18.8(re)-302.8(pr)20.9(es)19.9(er)19.7(ve)13.9(d)-306.4(i)0(n)]TJ
0 -1.1174 TD
[(hy)15.1(po)20.8(th)20.7(er)19.7(mi)19.5(c)-238.4(c)0(o)19.7(n)0(d)20.8(i)0(t)20.6(i)0(o)15(n)0(s)-226.6(\()0(r)20.9(e)0(f)19.7(r)0(i)20.7(g)0(e)19.7(r)0(a)14(t)0(e)19.5(d)-237.2(at)-222.4(4)]TJ
/F5 1 Tf
16.1157 0 TD
()Tj
/F4 1 Tf
.3859 0 TD
[(C\))14.1(:)-231.6(\()0(1)15.1(\))-231.5(Hi)19.8(be)13.9(rn)20.9(at)19.5(e-)19.7(A)]TJ
-16.5016 -1.1116 TD
[(\(G)14.2(ib)20.7(co)19.7(\),)-373.1(\(2)15.1(\))-381.2(D)0(M)19.1(E)0(M)-378.7(1)0(0)20.8(%)-382.3(FB)14.2(S,)-373(an)19.7(d)-387(\()0(3)20.9(\))-381.2(HT)13(S-)21(FR)20(S)-386.8(\()0(B)19.8(i)0(o)20.7(L)0(i)19.5(f)0(e)]TJ
T*
[(So)15.2(lu)20.7(ti)20.6(on)20.8(s\))15.3(.)-337.5(T)0(h)19.6(r)0(e)19.7(e)-336.3(pi)20.7(ec)12.8(es)-325.7(we)18.8(re)-331.6(pr)20.9(es)19.9(er)19.7(ve)13.9(d)-335.2(i)0(n)-330.6(c)0(r)19.7(y)0(o)20.8(g)0(e)19.7(n)0(i)20.7(c)-342.1(co)19.7(nd)20.8(i-)]TJ
0 -1.1174 TD
[(ti)14.8(on)20.8(s)-340.7(t)0(h)15(r)0(o)20.9(u)0(g)20.8(h)-346.7(co)19.7(nt)20.7(ro)20.9(ll)20.6(ed)-337.4(ra)19.7(te)-337.6(co)19.7(ol)20.7(in)20.7(g)-346.7(u)0(s)21(i)0(n)20.7(g)-346.7(\(4)20.9(\))-346.6(8)0(0)20.8(%)-342(DM)19.1(EM)]TJ
/F5 1 Tf
0 -1.1116 TD
(+)Tj
/F4 1 Tf
.5472 0 TD
[(10)20.8(%)-249.8(F)0(B)20(S)]TJ
/F5 1 Tf
4.0606 0 TD
(+)Tj
/F4 1 Tf
.5414 0 TD
[(10)20.8(%)-255.6(D)0(M)19.1(S)0(O)20.1(,)-251.1(\(5)20.9(\))-248.7(6)0(0)20.8(%)-249.8(DM)19.1(EM)]TJ
/F5 1 Tf
12.1818 0 TD
(+)Tj
/F4 1 Tf
.5414 0 TD
[(30)15.1(%)-249.8(F)0(B)20(S)]TJ
/F5 1 Tf
4.0664 0 TD
(+)Tj
/F4 1 Tf
.5414 0 TD
[(10)20.8(%)]TJ
-22.4801 -1.1116 TD
[(DM)13.3(SO)20.1(,)-302.9(a)0(n)13.9(d)-306.4(\(6)20.9(\))-306.3(9)0(0)20.8(%)-307.4(HT)18.8(S-)21(FR)14.2(S)]TJ
/F5 1 Tf
12.9421 0 TD
(+)Tj
/F4 1 Tf
.5414 0 TD
[(10)20.8(%)-307.4(D)0(M)19.1(S)0(O)-296.7(\()-75.9(Mr)20(.)-302.9(F)0(r)15.3(o)0(s)21(t)0(y)]TJ
/F5 1 Tf
9.924 0 TD
()Tj
/F4 1 Tf
-23.4074 -1.1174 TD
[(fr)15.1(ee)18.5(zi)19.5(ng)-295.9(co)13.9(nt)20.7(ai)19.5(ne)19.7(r)-312.1(f)0(o)20.9(r)-306.3(1)-312.1(d)0(a)19.7(y)-306.4(in)-301.8(a)]TJ
/F5 1 Tf
13.3395 0 TD
(-)Tj
/F4 1 Tf
.5414 0 TD
(80)Tj
/F5 1 Tf
.9792 0 TD
()Tj
/F4 1 Tf
.3859 0 TD
-.018 Tc
[(C)-325.4(f)-18(re)-18(ez)-18(er)-18(,)-316.2(f)-18(ol)-18(lo)-18(we)-18(d)-315.1(b)-18(y)]TJ
-15.2459 -1.1116 TD
0 Tc
[(2)-335.2(d)0(a)13.9(y)0(s)-324.6(i)0(n)-324.9(l)0(i)20.6(q)0(u)20.8(i)0(d)-324.9(n)0(i)20.7(t)0(r)20.7(o)0(g)15.1(e)0(n)19.7(\))0(.)-321.3(F)0(r)15.3(e)0(s)19.9(h)-335.2(ge)19.7(ls)-324.7(no)20.8(t)-335.3(e)0(x)19.7(p)0(o)20.8(s)0(e)14.1(d)-329.4(to)-324.9(pr)15.1(es)19.9(er)19.7(-)]TJ
T*
[(va)13.9(ti)20.6(on)-244.1(co)19.7(nd)20.8(it)20.6(io)20.7(ns)-243.9(we)18.8(re)-245.2(us)21(ed)-245.3(as)-245.1(co)19.7(nt)20.7(ro)20.9(ls)15.1(.)-251.1(T)0(h)19.6(e)0(s)19.9(e)-255.7(we)18.8(re)-245.2(an)19.7(al)19.6(yz)19.7(ed)]TJ
T*
[(im)13.7(m)15(e)0(d)13.9(i)0(a)19.5(t)0(e)19.6(l)0(y)-497.7(b)0(y)-497.5(l)0(i)14.8(v)0(e)19.7(/)0(d)20.7(e)0(a)18.5(d)-507.9(as)14.1(sa)19.9(y)-507.9(u)0(s)21(i)0(n)20.7(g)-513.7(Sy)21(to)20.7(-9)-497.5(\(5)]TJ
/F11 1 Tf
20.4412 0 TD
(m)Tj
/F4 1 Tf
.5414 0 TD
[(M;)-498.6(Li)19.5(fe)]TJ
-20.9826 -1.1174 TD
[(Te)12.7(ch)19.7(no)20.8(lo)20.7(gi)20.7(es)19.9(\))-686.5(a)0(n)19.7(d)-680.7(pr)20.9(op)20.8(id)20.7(iu)15(m)-682(i)0(o)20.7(d)0(i)20.7(d)0(e)-671.5(\()0(2)20.9(0)]TJ
/F11 1 Tf
17.8032 0 TD
(m)Tj
/F4 1 Tf
.5472 0 TD
[(g/)20.7(mL)18.4(;)-680.9(S)0(i)20.9(g)0(m)19.6(a)0(-)]TJ
-18.3504 -1.1116 TD
[(Al)14.1(dr)20.9(ic)19.6(h,)-194.6(Un)20(it)20.6(ed)-199.2(Ki)19.8(ng)20.8(do)20.8(m\))13.9(,)-205(r)0(e)19.7(s)0(p)21(e)0(c)18.5(t)0(i)20.6(v)0(e)19.7(l)0(y)15(,)-205(di)20.7(lu)20.7(te)19.5(d)-208.4(i)0(n)-192.4(c)0(e)18.5(l)0(l)-198.3(c)0(u)19.7(l)0(t)20.6(u)0(r)20.9(e)]TJ
T*
[(me)18.4(di)14.9(a,)-362.8(an)13.9(d)-375.5(i)0(n)20.7(c)0(u)19.7(b)0(a)19.7(t)0(e)19.5(d)-381.2(at)-366.4(ro)20.9(om)-366.3(te)19.5(mp)19.6(er)19.7(at)13.8(ur)20.9(e)-376.6(f)0(o)20.9(r)-375.4(1)-156.6(m)0(i)13.7(n)-375.5(be)19.7(fo)20.9(re)]TJ
0 -1.1174 TD
[(vi)14.9(ew)18.8(in)20.7(g.)-327.1(Pr)15.3(es)19.9(er)19.7(ve)19.7(d)-340.9(s)0(a)19.9(m)0(p)19.6(l)0(e)19.6(s)-340.7(we)18.8(re)-331.6(ra)19.7(pi)20.7(dl)20.7(y)-340.9(w)0(a)18.8(r)0(m)19.6(e)0(d)-331.7(i)0(n)-324.9(a)-342.1(37)]TJ
/F5 1 Tf
23.2404 0 TD
()Tj
/F4 1 Tf
.3859 0 TD
(C)Tj
-23.6263 -1.1116 TD
[(wa)13(te)19.5(r)-410(b)0(a)19.7(t)0(h)-405.5(a)0(f)19.7(t)0(e)19.6(r)-410(th)20.7(e)-417(p)0(r)20.9(e)0(s)19.9(e)0(r)19.7(v)0(a)19.7(t)0(i)14.8(o)0(n)-399.6(p)0(e)19.7(r)0(i)20.7(o)0(d)-405.4(a)0(n)19.7(d)-410(th)20.7(en)-406.5(li)20.6(ve)19.7(/d)20.7(ea)18.5(d)]TJ
T*
[(as)14.1(sa)19.9(y)-179.6(p)0(e)19.7(r)0(f)15.1(o)0(r)20.9(m)0(e)18.4(d)-179.6(as)-176(de)19.7(sc)19.9(ri)20.7(be)19.7(d)-185.4(a)0(b)19.7(o)0(v)20.8(e)0(.)-172.7(F)0(u)21(r)0(t)20.7(h)0(e)13.9(r)-179.6(ex)19.7(pe)19.7(ri)20.7(me)18.5(nt)14.9(s)-179.4(w)0(e)18.8(r)0(e)]TJ
0 -1.1174 TD
[(co)13.9(nd)20.8(uc)19.7(te)19.6(d)-289.1(o)0(n)-278.7(u)0(n)20.8(a)0(l)19.6(i)0(g)20.7(n)0(e)19.7(d)-289.1(ge)19.7(ls)-278.6(an)19.7(d)-289.1(E)0(n)19.6(g)0(N)20(T)-290.3(us)21(in)14.9(g)-283.3(t)0(h)15(e)-284.5(fo)15.1(ll)20.6(ow)20(in)20.7(g)]TJ
0 -1.1116 TD
[(pr)15.1(es)19.9(er)19.7(va)19.7(ti)20.6(on)-169.2(co)19.7(nd)20.8(it)20.6(io)20.7(ns)15.3(:)-174(H)0(i)19.8(b)0(e)19.7(r)0(n)20.9(a)0(t)13.8(e)0(-)19.7(A)0(,)-166.7(H)0(T)18.8(S)0(-)21(F)0(R)20(S)0(,)-165.6(6)0(0)20.8(%)-174.9(DM)19.1(EM)]TJ
/F5 1 Tf
T*
(+)Tj
/F4 1 Tf
.5472 0 TD
[(30)20.8(%)-295.9(F)0(B)20(S)]TJ
/F5 1 Tf
4.1527 0 TD
(+)Tj
/F4 1 Tf
.5472 0 TD
[(10)20.8(%)-295.9(D)0(M)19.1(S)0(O)20.1(,)-297.2(an)19.7(d)-294.8(9)0(0)15.1(%)-295.9(HT)18.8(S-)21(FR)20(S)]TJ
/F5 1 Tf
13.5526 0 TD
(+)Tj
/F4 1 Tf
.5414 0 TD
[(10)20.8(%)-295.9(D)0(M)19.1(S)0(O)]TJ
-19.3411 -1.1174 TD
[(\(u)15.1(na)19.7(li)20.6(gn)20.8(ed)-302.9(ge)19.7(ls)-301.6(on)20.8(ly)15(\))-312.1(u)0(s)21(i)0(n)20.7(g)-312.1(th)20.7(e)-313.3(m)0(e)18.4(t)0(h)20.7(o)0(d)20.8(s)-311.9(de)19.7(sc)14.1(ri)20.7(be)19.7(d)-312.1(a)0(b)19.7(o)0(v)20.8(e)-313.3(fo)20.9(r)]TJ
0 -1.1116 TD
[(du)15.1(ra)19.7(ti)20.6(on)20.8(s)-185.2(o)0(f)-180.7(2)0(,)-177.3(\()0(E)19.7(n)0(g)20.8(N)0(T)-177.1(o)0(n)20.8(l)0(y)20.7(\))0(,)-177.3(7)0(,)-177.3(a)0(n)19.7(d)-185.4(14)-175(da)13.9(ys)-174.8(at)-176.3(4)]TJ
/F5 1 Tf
18.7939 0 TD
()Tj
/F4 1 Tf
.3859 0 TD
.1864 Tc
[(Co)186.4(r)5.7(i)186.4(n)11.3(l)186.4(i)207(q)186.4(u)207.2(i)186.4(d)]TJ
-19.1798 -1.1116 TD
0 Tc
[(ni)14.9(tr)20.7(og)20.8(en)19.7(.)-210.8(S)0(a)19.8(m)0(p)19.6(l)0(e)19.6(s)-208.3(st)20.9(or)20.9(ed)-199.2(in)-198.2(li)20.6(qu)20.8(id)-198.2(ni)20.7(tr)15(og)20.8(en)-199.2(we)18.8(re)-199.2(r)21(st)-198(co)19.7(ol)20.7(ed)-199.2(to)]TJ
/F5 1 Tf
T*
(-)Tj
/F4 1 Tf
.5472 0 TD
(80)Tj
/F5 1 Tf
.9792 0 TD
()Tj
/F4 1 Tf
.3859 0 TD
[(C)-318.9(\()-70.2(Mr)20(.)-320.2(F)0(r)21(o)0(s)21(t)0(y)-307.6(f)0(r)15.1(e)0(e)18.5(z)0(i)19.5(n)0(g)-307.5(c)0(o)19.7(n)0(t)20.7(a)0(i)19.5(n)0(e)13.9(r)-312.1(fo)15.1(r)-317.8(1)-317.9(da)19.7(y\))20.9(.)-314.4(F)0(o)15.2(r)-317.8(te)19.5(st)20.9(in)20.7(g)]TJ
-1.9122 -1.1174 TD
[(al)13.8(ig)20.7(ne)19.7(d)-208.4(c)0(e)18.5(l)0(l)20.6(u)0(l)14.9(a)0(r)-199.2(g)0(e)19.7(l)0(s)20.9(,)-210.8(En)19.6(gN)20(T)-209.6(c)0(o)13.9(n)0(s)21(t)0(r)20.7(u)0(c)19.7(t)0(s)-203.7(w)0(e)18.8(r)0(e)-199.2(c)0(u)19.7(t)-208.6(in)20.7(to)-203.9(qu)20.8(ar)19.7(te)19.6(rs)15.3(,)]TJ
0 -1.1116 TD
[(un)15.1(le)19.6(ss)-249.5(ot)20.7(he)19.7(rw)14.2(is)20.9(e)-261.4(s)0(t)20.9(a)0(t)19.5(e)0(d)19.7(.)]TJ
/F10 1 Tf
8.9663 0 0 8.9663 313.0582 248.3716 Tm
[(Metabolic)-338.8(assessment)-330.7(of)-336.7(EngNT)]TJ
/F4 1 Tf
9.843 0 0 9.843 323.0361 231.9306 Tm
[(Ce)18.6(ll)20.6(Ti)13.8(te)19.5(r-)20.9(Gl)19.8(o)]TJ
/F5 1 Tf
7.3823 0 0 6.5614 375.5905 236.296 Tm
()Tj
/F4 1 Tf
9.843 0 0 9.843 385.455 231.9306 Tm
[(\(P)15.3(ro)20.9(me)18.4(ga)19.7(,)-406.6(U)0(n)20(i)0(t)14.8(e)0(d)-395(K)0(i)19.8(n)0(g)15.1(d)0(o)20.8(m)0(\))-395.1(w)0(a)18.8(s)-409.8(us)21(ed)-400.8(to)]TJ
-7.3551 -1.1174 TD
[(qu)15.1(an)19.7(ti)20.6(fy)-163.4(th)20.7(e)-169.3(p)0(r)20.9(e)0(s)19.9(e)0(n)13.9(c)0(e)-160(o)0(f)-157.7(v)0(i)15(a)0(b)19.7(l)0(e)-159(S)0(c)19.8(h)0(w)14.2(a)0(n)19.7(n)-168.1(ce)18.5(ll)20.6(s)-173.7(i)0(n)-157.8(E)0(n)19.6(g)0(N)20(T)-175.1(be)19.7(fo)20.9(re)]TJ
0 -1.1116 TD
[(an)13.9(d)-214.2(a)0(f)19.7(t)0(e)19.6(r)-219.9(pr)20.9(es)19.9(er)19.7(va)13.9(ti)20.6(on)-203.8(in)-209.7(Hi)19.8(be)19.7(rn)20.9(at)13.8(e-)19.7(A)-215.1(f)0(o)20.9(r)-219.9(3)-214.2(d)0(a)19.7(y)0(s)15.3(.)-216.5(Ea)18.5(ch)-204.9(En)19.6(gN)20(T)]TJ
T*
[(sa)14.1(mp)19.6(le)-280(wa)18.8(s)-288.9(c)0(u)19.7(t)-289.2(tr)15(an)19.7(sv)21(er)19.7(se)19.9(ly)-284.5(in)20.7(to)-278.8(tw)19.8(o)-289.1(e)0(q)19.7(u)0(a)19.7(l)-295(pi)20.7(ec)18.5(es)19.9(,)-291.4(e)0(a)18.5(c)0(h)-279.8(c)0(o)19.7(n)0(-)]TJ
0 -1.1174 TD
[(ta)13.8(in)20.7(in)20.7(g)-329.4(0)0(.)18.5(8)]TJ
/F5 1 Tf
4.4523 0 TD
()Tj
/F4 1 Tf
.7084 0 TD
(10)Tj
7.3823 0 0 6.5614 373.4928 192.4724 Tm
(6)Tj
9.843 0 0 9.843 380.466 188.0503 Tm
[(ce)18.5(ll)20.6(s,)-321.1(on)20.8(e)-336.3(o)0(f)-319(w)0(h)20(i)0(c)19.5(h)-329.4(wa)13(s)-329.2(t)0(e)19.6(s)0(t)20.9(e)0(d)-325.9(i)0(m)19.5(m)0(e)18.4(d)0(i)20.7(a)0(t)19.5(e)0(l)19.5(y)]TJ
-6.8483 -1.1116 TD
[(\(b)15.1(as)19.9(el)19.5(in)20.7(e)-313.3(c)0(o)19.7(n)0(t)14.9(r)0(o)20.9(l)0(\))-301.8(a)0(n)19.7(d)-312.1(th)20.7(e)-313.3(o)0(t)14.9(h)0(e)19.7(r)-312.1(su)21(bj)20.7(ec)18.5(te)13.8(d)-312.1(t)0(o)-301.8(p)0(r)20.9(e)0(s)19.9(e)0(r)19.7(v)0(a)13.9(t)0(i)20.6(o)0(n)-301.7(f)0(o)20.9(r)]TJ
T*
.3236 Tc
[(3d)323.6(a)343.3(y)323.6(s)10.6(a)323.6(t)9.1(4)]TJ
/F5 1 Tf
4.4811 0 TD
0 Tc
()Tj
/F4 1 Tf
.3859 0 TD
[(C)-324.7(i)0(n)-313.4(H)0(i)19.8(b)0(e)19.7(r)0(n)15.1(a)0(t)19.5(e)0(-)19.7(A)-324.5(or)-313.2(DM)19.1(EM)-309.6(10)15.1(%)-318.9(F)0(B)20(S)0(,)-315.4(t)0(h)20.7(e)0(n)-314.4(r)0(e)19.7(-)]TJ
-4.8669 -1.1116 TD
[(tu)15(rn)20.9(ed)-170.4(to)-163.6(st)20.9(an)13.9(da)19.7(rd)-163.4(cu)13.9(lt)20.6(ur)20.9(e)-180.8(c)0(o)19.7(n)0(d)20.8(i)0(t)20.6(i)0(o)20.7(n)0(s)-169(\()0(3)20.9(7)]TJ
/F5 1 Tf
15.6203 0 TD
()Tj
/F4 1 Tf
.3859 0 TD
.1807 Tc
[(C5)180.7(%)16.2(C)180.7(O)]TJ
7.3823 0 0 6.5614 507.1747 153.5811 Tm
0 Tc
(2)Tj
9.843 0 0 9.843 512.6172 155.2252 Tm
[(in)-163.6(DM)19.1(EM)]TJ
-20.2742 -1.1174 TD
[(10)15.1(%)-411.1(F)0(B)20(S)0(\))-399.4(f)0(o)20.9(r)-415.8(1)-410(o)0(r)-399.6(3)-410(da)19.7(ys)21(,)-412.4(t)0(h)20.7(e)0(n)-406.5(t)0(e)19.6(s)0(t)20.9(e)0(d)19.7(.)-412.4(Eq)19.6(ui)20.7(va)19.7(le)19.6(nt)-405.5(co)19.7(nt)20.7(ro)20.9(l)]TJ
0 -1.1116 TD
[(sa)14.1(mp)19.6(le)19.6(s)-185.2(w)0(e)18.8(r)0(e)-176.1(m)0(a)18.4(i)0(n)20.7(t)0(a)19.6(i)0(n)20.7(e)0(d)-176.2(u)0(n)20.8(d)0(e)19.7(r)-185.4(st)15.1(an)19.7(da)19.7(rd)-175(cu)19.7(lt)20.6(ur)20.9(e)-186.6(c)0(o)19.7(n)0(d)15.1(i)0(t)20.6(i)0(o)20.7(n)0(s)-174.8(f)0(o)20.9(r)]TJ
T*
[(th)15(e)-307.5(e)0(n)19.7(t)0(i)14.8(r)0(e)-302.8(d)0(u)20.8(r)0(a)19.7(t)0(i)20.6(o)0(n)-301.7(\()0(4)-301.7(o)0(r)-301.7(6)-306.4(da)13.9(ys)21(\).)-298.2(On)20(e)-313.3(h)0(u)20.8(n)0(d)20.8(r)0(e)14(d)-306.4(mi)19.5(cr)14(ol)20.7(it)20.6(er)19.7(s)-311.9(o)0(f)]TJ
0 -1.1174 TD
[(Ce)12.9(ll)20.6(Ti)19.5(te)19.6(r-)20.9(Gl)19.8(o)-168.1(a)0(n)19.7(d)-168.1(10)20.8(0)]TJ
/F11 1 Tf
8.749 0 TD
(m)Tj
/F4 1 Tf
.5472 0 TD
[(L)-163.6(o)0(f)-157.7(D)0(M)19.1(E)0(M)-159.8(1)0(0)20.8(%)-169.2(FB)20(S)-168(a)0(l)19.5(o)0(n)20.8(g)-168.1(wi)19.8(th)-157.8(ea)18.5(ch)]TJ
-9.2962 -1.1116 TD
[(sa)14.1(mp)19.6(le)-170.5(we)18.8(re)-170.4(ad)19.7(de)19.7(d)-185.4(t)0(o)-169.4(e)0(a)18.5(c)0(h)-170.4(w)0(e)18.8(l)0(l)-169.5(o)0(f)-169.2(a)0(n)-176.2(o)0(p)20.8(a)0(q)19.7(u)0(e)-170.4(9)0(6)20.8(-)0(w)20(e)0(l)13.8(l)-179.8(pl)20.7(at)19.5(e)-180.8(a)0(n)19.7(d)]TJ
T*
[(pl)14.9(ac)18.5(ed)-377.7(on)-370.8(a)-388.2(s)0(h)21(a)0(k)13.9(e)0(r)-377.7(a)0(t)-372.1(1)0(0)15.1(0)-156.6(rp)20.9(m.)-374.4(Af)20(te)19.5(r)-387(1)0(0)-146.2(m)0(i)19.5(n)0(,)-378.9(t)0(h)20.7(e)-388.2(pl)20.7(at)19.5(e)-388.2(w)0(a)18.8(s)]TJ
0 -1.1174 TD
[(in)15(cu)19.7(ba)19.7(te)19.6(d)-306.4(s)0(t)15.1(a)0(t)19.6(i)0(c)19.5(a)0(l)19.5(l)0(y)-296.1(f)0(o)20.9(r)-312.1(a)-307.5(f)0(u)20.9(r)0(t)20.7(h)0(e)19.7(r)-312.1(20)-146.2(mi)19.5(n)-306.4(a)0(t)-297.2(r)0(o)20.9(o)0(m)-297.2(t)0(e)13.8(m)0(p)19.6(e)0(r)19.7(a)0(t)19.5(u)0(r)20.9(e)]TJ
0 -1.1116 TD
[(\(2)15.1(1)]TJ
/F5 1 Tf
1.3075 0 TD
()Tj
/F4 1 Tf
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-.0197 Tc
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/F4 1 Tf
9.843 0 0 9.843 69.7323 261.1842 Tm
[(Scanning)-333.7(electron)-335.1(microscopy)-331.3(\(SEM\))-329.9(was)-333.4(used)-333.7(to)-330.6(assess)]TJ
-1.0137 -1.1174 TD
[(whether)-350.8(EngNT)-342.8(preserved)-347.2(in)-347.9(Hibernate-A)-345.9(or)-342(60%)-344.2(DMEM,)]TJ
0 -1.1116 TD
[(30%)-459.4(FBS,)-462.5(and)-459.5(10%)-459.4(DMSO)-461.9(for)-464(3)-456.1(days)-460.4(affected)-462.8(collagen)]TJ
T*
[(alignment)-584.1(compared)-589.5(with)-581.5(EngNT)-584.7(maintained)-586.3(in)-578.3(normal)]TJ
0 -1.1174 TD
[(culture)-511.4(conditions)-513.5(for)-504.3(3)-507.9(days)-506.5(\(no)-510.1(preservation\).)-510.8(Samples)]TJ
0 -1.1116 TD
[(were)-238(xed)-235.8(in)-238.5(3%)-233.6(glutaraldehyde)-239.3(for)-233.6(at)-239.6(least)-238.3(24)-163.5(h)-237.2(a)0(t)-233.9(4)]TJ
/F5 1 Tf
21.0172 0 TD
()Tj
/F4 1 Tf
.3974 0 TD
[(C,)-236(then)]TJ
-21.4146 -1.1116 TD
[(dehydrated)-377.5(through)-370.7(an)-372(increasing)-371.6(graded)-371.8(series)-377.3(of)-370.8(ethanol.)]TJ
0 -1.1174 TD
[(Finally,)-369.6(samples)-368.1(were)-364.7(fully)-368.6(immersed)-364.8(in)-365.2(hexamethyldisila-)]TJ
0 -1.1116 TD
[(zane)-301.6(for)-302.7(2)-162.4(min,)-301.8(which)-301.4(was)-298.9(then)-299.4(removed,)-302.7(and)-298.2(samples)-304.8(were)]TJ
T*
[(left)-483.8(to)-474.6(dry.)-479(Samples)-483.3(were)-479.9(mounted)-476.8(onto)-476.8(aluminum)-485(SEM)]TJ
T*
[(stubs)-258.7(using)-253.1(double-sided)-257.5(carbon)-256.6(sticky)-255.5(tabs)-258.9(\(Agar)-255.1(Scientic,)]TJ
0 -1.1174 TD
[(United)-371.8(Kingdom\),)-365.8(sputter)-371.8(coated)-367.3(with)-368.4(gold)-361.6(palladium,)-368.6(and)]TJ
0 -1.1116 TD
[(imaged)-338.6(using)-333.7(a)-336.3(Philips)-330.3(XL30)-334.8(FEGSEM)-338.8(at)-331.8(5)-168.1(kV.)]TJ
/F10 1 Tf
8.9663 0 0 8.9663 59.7543 105.9023 Tm
[(Microscopy)-334.5(and)-335.7(image)-334.3(analysis)]TJ
/F4 1 Tf
9.843 0 0 9.843 69.7323 89.4614 Tm
[(Samples)-322.1(were)-318.6(placed)-321.2(onto)-315.6(microscope)-320.9(slides)-318.7(and)-315.5(imaged)]TJ
-1.0137 -1.1174 TD
[(immediately)-398.7(after)-399.6(live/dead)-393.9(staini)-8(ng)-393.9(using)-391.3(a)-393.9(2)0(0)]TJ
/F5 1 Tf
19.8883 0 TD
()Tj
/F4 1 Tf
.72 0 TD
(objective)Tj
-20.6082 -1.1116 TD
[(on)-376.6(a)-370.9(Zeiss)-376.2(Axioskop)-378.2(uorescence)-380.4(microscope.)-376.2(Five)-374.2(equiv-)]TJ
T*
[(alent)-405.5(predetermi)-10.2(ned)-401.9(elds)-403.9(were)-405(assesse)-10.4(d)-398.5(o)0(n)-405.4(each)-405.3(piece)-406.5(of)]TJ
25.5097 67.9242 TD
[(un)20.8(al)19.5(ig)20.7(ne)19.7(d)-340.9(g)0(e)19.7(l)-341(to)-330.6(n)15.2(d)-340.9(t)0(h)20.7(e)-342.1(me)18.4(an)-331.7(ce)18.5(ll)-330.8(de)19.7(at)19.6(h)-340.9(p)0(e)19.7(r)0(c)19.7(e)0(n)19.7(t)0(a)19.5(g)0(e)13.9(.)-337.5(Se)19.8(ve)19.7(n)]TJ
0 -1.1174 TD
[(pr)20.9(ed)19.7(et)19.5(er)19.7(mi)13.7(ne)19.7(d)-237.2()0(e)19.8(l)0(d)20.7(s)-237(we)13(re)-228(as)19.9(se)19.9(ss)15.5(ed)-228(fo)20.9(r)-237.2(e)0(a)18.5(c)0(h)-228(q)0(u)15.1(a)0(r)19.7(t)0(e)19.5(r)0(e)19.7(d)-237.2(En)19.6(gN)14.2(T)]TJ
0 -1.1116 TD
[(sa)19.9(mp)19.6(le)19.6(.)]TJ
1.0137 -1.1116 TD
[(Schwann)-523.4(cell)-525.4(and)-522.8(neurite)-528.6(alignment)-526.5(and)-522.8(growth)-525.9(were)]TJ
-1.0137 -1.1116 TD
[(assessed)-374.4(using)-374.1(confocal)-381(microscopy)-371.6(\(LSM710;)-379.5(Zeiss,)-373.9(Ger-)]TJ
0 -1.1174 TD
[(many\))-434.1(by)-428.4(tile)-432.2(scanning)-437.3(the)-430.8(entire)-435.4(quarter)-436.3(of)-428.4(each)-434.1(EngNT)]TJ
0 -1.1116 TD
[(sheet.)-241.6(Images)-240.3(were)-243.7(captured)-242.8(using)-241.6(a)-238.4(2)0(0)]TJ
/F5 1 Tf
15.8334 0 TD
()Tj
/F4 1 Tf
.7142 0 TD
[(lens,)-245.1(using)-241.6(z-stacks)]TJ
-16.5476 -1.1116 TD
[(of)-376.5(18)]TJ
/F11 1 Tf
2.373 0 TD
(m)Tj
/F4 1 Tf
.5529 0 TD
[(m)-376.7(for)-377.6(neurites)-379.8(and)-373.1(25)]TJ
/F11 1 Tf
9.2443 0 TD
(m)Tj
/F4 1 Tf
.5529 0 TD
[(m)-376.7(for)-377.6(Schwann)-379.4(cells)-376.5(with)-374.2(a)]TJ
-12.7232 -1.1174 TD
[(step)-333.8(size)-335(of)-336.2(2)]TJ
/F11 1 Tf
5.6733 0 TD
(m)Tj
/F4 1 Tf
.5529 0 TD
[(m.)-334.1(Alignment)-336.1(analysis)-339.3(was)-333.5(conducted)-336.1(using)]TJ
-6.2262 -1.1116 TD
(Volocity)Tj
/F5 1 Tf
3.5077 0 TD
()Tj
/F4 1 Tf
1.1865 0 TD
[(software)-305.6(\(Perkin)-302.5(Elmer,)-300.6(Waltham,)-305.2(MA\))-298.8(running)]TJ
-4.6942 -1.1116 TD
[(automated)-245.3(3D)-239.2(image)-245.3(analysis)-247.2(protocols)-247.3(to)-238.5(measure)-248.3(the)-240.7(angle)]TJ
0 -1.1174 TD
[(of)-330.5(Schwann)-339.1(cell)-335.3(and)-332.8(neurite)-338.6(alignment)-336.4(in)-330.6(each)-336.2(eld.)]TJ
7.3823 0 0 6.5614 522.8786 608.9951 Tm
(21)Tj
9.843 0 0 9.843 320.8251 593.6313 Tm
[(St)20.9(at)19.6(is)20.9(ti)20.6(ca)18.5(l)-346.8(a)0(n)19.7(a)0(l)19.5(y)0(s)15.3(i)0(s)-336.2(w)0(a)18.8(s)-346.5(ca)18.5(rr)20.9(ie)19.6(d)-346.7(o)0(u)20.8(t)-346.8(us)21(in)20.7(g)-346.7(a)0(n)19.7(a)0(l)19.5(y)0(s)21(i)0(s)-336.2(o)0(f)-336.2(v)0(a)19.7(r)0(i)20.7(-)]TJ
-1.0137 -1.1116 TD
[(an)19.7(ce)-281(wi)19.8(th)-278.8(T)15(u)0(k)15.1(e)0(y)19.7()0(s)-278.4(o)0(r)-278.6(D)0(u)20(n)0(n)20.8(e)0(t)19.6(t)0()20.7(s)-288.9(po)20.8(st)20.9(-t)15(es)19.9(ts)-278.6(\(G)20(ra)19.7(ph)20.8(Pa)14.1(d)-283.3(P)0(r)15.3(i)0(s)20.9(m)0(\))19.6(.)]TJ
/F7 1 Tf
8.9663 0 0 8.9663 310.8472 552.529 Tm
(Results)Tj
/F10 1 Tf
0 -1.8336 TD
[(Survival)-337(of)-330.4(Schwann)-334.1(cells)-332.5(in)-336.5(preserved)-335.2(collagen)-331.7(gels)]TJ
/F4 1 Tf
9.843 0 0 9.843 320.8251 519.6471 Tm
[(An)-360.2(initial)-360.9(panel)-365.1(of)-359.3(preservation)-362.3(conditions)-363.7(was)-362.2(tested)-366.1(to)]TJ
-1.0137 -1.1174 TD
[(determine)-539(what)-536.5(medium)-540.3(formulation)-539(and)-534.4(method)-539(\(h)20.9(yp)20.8(o-)]TJ
0 -1.1116 TD
[(th)20.7(er)19.7(mi)19.5(c)-417(o)0(r)-399.6(c)0(r)19.7(y)0(o)20.8(g)0(e)13.9(n)0(i)20.7(c)0(\))-400.7(m)0(i)19.5(n)0(i)20.7(m)0(i)19.5(z)0(e)12.8(d)-410(ce)18.5(ll)-399.9(de)13.9(at)19.6(h)-410(o)0(p)20.8(t)0(i)14.8(m)15(al)13.8(ly)-399.7(af)19.7(te)19.5(r)]TJ
T*
[(2)-225.7(d)0(a)19.7(y)0(s)-215.1(w)0(i)14.1(t)0(h)20.7(i)0(n)-215.4(u)0(n)20.8(a)0(l)19.5(i)0(g)14.9(n)0(e)19.7(d)-225.7(co)19.7(ll)20.6(ag)19.7(en)-216.5(ge)13.9(ls)-215.2(co)19.7(mp)19.6(ar)19.7(ed)-216.5(wi)14.1(th)-215.4(ba)19.7(se)19.9(li)20.6(ne)]TJ
0 -1.1174 TD
[(co)19.7(nt)20.7(ro)20.9(l)-271.9(g)0(e)19.7(l)0(s)-261.3(\()0(F)21(i)0(g)20.7(.)-268.4(1A)20(\).)-263.7(Ni)19.8(ne)19.7(ty)-261.5(pe)19.7(rc)19.7(en)19.7(t)-266.2(H)0(T)13(S)-265.9(FR)20(S)-271.6(1)0(0)20.8(%)-267.1(DM)19.1(SO)]TJ
0 -1.1116 TD
[(wa)18.8(s)-346.5(i)0(n)20.7(c)0(l)19.5(u)0(d)20.8(e)0(d)-337.4(b)0(e)13.9(c)0(a)18.5(u)0(s)21(e)-347.8(of)-336.2(it)20.6(s)-346.5(s)0(i)20.9(m)0(i)19.5(l)0(a)13.8(r)-340.9(fo)15.1(rm)19.6(ul)20.7(at)19.5(io)20.7(n)-346.7(t)0(o)-336.4(t)0(h)20.7(e)-347.8(co)19.7(m-)]TJ
T*
[(me)18.4(rc)19.7(ia)19.6(l)-450.5(p)0(r)20.9(o)0(d)20.8(u)0(c)19.7(t)-450.5(Cr)19.8(yo)15.1(st)20.9(or)]TJ
/F5 1 Tf
7.3823 0 0 6.5614 412.044 458.2487 Tm
()Tj
/F4 1 Tf
9.843 0 0 9.843 417.8267 453.8834 Tm
[(.)-446.9(P)0(r)21(e)0(l)13.8(i)0(m)19.5(i)0(n)20.7(a)0(r)19.7(y)-450.4(da)19.7(ta)-441.2(in)20.7(di)20.7(ca)18.5(te)19.5(d)-450.4(t)0(h)20.7(a)0(t)]TJ
-10.8686 -1.1174 TD
[(in)20.7(cr)19.7(ea)18.5(si)15.1(ng)-445.7(th)20.7(e)-463(c)0(o)19.7(n)0(c)19.7(e)0(n)19.7(t)0(r)15(a)0(t)19.5(i)0(o)20.7(n)-461.9(of)-445.7(FB)14.2(S)-455.9(i)0(n)-451.6(t)0(h)20.7(e)-463(fo)20.9(rm)19.6(ul)20.7(at)19.5(io)15(n)-456.1(o)0(f)]TJ
0 -1.1116 TD
[(cr)19.7(yo)20.8(ge)19.7(ni)20.7(c)-249.9(m)0(e)18.4(d)0(i)20.7(a)-244.2(wa)13(s)-242.8(n)0(o)20.8(t)-248.9(be)19.7(ne)19.7(c)19.8(ia)19.6(l)-248.9(t)0(o)-232.7(c)0(e)18.5(l)0(l)-238.6(s)0(u)21(r)0(v)20.9(i)0(v)15(a)0(l)19.5(,)-245.3(wi)19.8(th)-232.7(25)15.1(%)]TJ
T*
[(DM)19.1(EM)18.7(,)-285.7(6)0(5)20.8(%)-284.4(FB)20(S,)-275.1(an)19.7(d)-289.1(1)0(0)20.8(%)-284.4(DM)19.1(SO)-279.4(an)19.7(d)-283.3(9)0(0)20.8(%)-284.4(FB)14.2(S)-283.2(a)0(n)19.7(d)-283.3(10)15.1(%)]TJ
T*
[(DM)19.1(SO)-498.2(re)14(su)21(lt)20.6(in)20.7(g)-513.7(i)0(n)-497.7(3)0(8)15.1(.)13.9(8%)-504.4(an)19.7(d)-507.9(3)0(7)15.1(.)13.9(5%)-504.4(ce)18.5(ll)-497.8(de)13.9(at)19.5(h,)-499.9(re)19.7(sp)21(ec)18.5(-)]TJ
0 -1.1174 TD
[(ti)20.6(ve)19.7(ly)20.7(,)-302.9(a)0(f)19.7(t)0(e)19.6(r)-306.3(2)-300.6(d)0(a)19.7(y)0(s)21(.)-302.9(Sa)19.8(mp)19.6(le)13.8(s)-300.4(p)0(r)20.9(e)0(s)19.9(e)0(r)14(v)0(e)19.7(d)-300.6(hy)20.8(po)20.8(th)20.7(er)14(mi)19.5(ca)18.5(ll)20.6(y)-300.6(w)0(i)19.8(t)0(h)]TJ
0 -1.1116 TD
[(Hi)19.8(be)19.7(rn)20.9(at)19.5(e-)14(A)-330.3(a)0(n)19.7(d)-329.4(HT)18.8(S-)21(FR)20(S)-329.2(r)0(e)14(s)0(u)21(l)0(t)20.6(e)0(d)-320.1(i)0(n)-319.1(l)0(o)20.7(w)0(e)13(r)-329.4(ce)18.5(ll)-319.2(de)19.7(at)19.5(h)-329.4(t)0(h)20.7(a)0(n)]TJ
T*
[(al)19.5(l)-415.9(o)0(t)20.7(h)0(e)19.7(r)-421.5(co)19.7(nd)20.8(it)20.6(io)20.7(ns)15.3(.)-412.4(H)0(i)14.1(b)0(e)19.7(r)0(n)20.9(a)0(t)19.5(e)0(-)19.7(A)-416.7(sa)14.1(mp)19.6(le)19.6(s)-415.6(w)0(e)18.8(r)0(e)-406.5(n)0(o)15.1(t)-415.9(si)20.9(gn)20.8(i)20.9(-)]TJ
0 -1.1174 TD
[(ca)18.5(nt)20.7(ly)-296.1(di)20.7(ff)20.9(er)19.7(en)19.7(t)-306.5(i)0(n)-290.3(t)0(e)13.8(r)0(m)19.6(s)-300.4(of)-295.9(ce)18.5(ll)-296.2(de)19.7(at)19.5(h)-300.6(c)0(o)19.7(m)0(p)19.6(a)0(r)14(e)0(d)-291.3(w)0(i)19.8(t)0(h)-296.1(c)0(o)19.7(n)0(t)20.7(r)0(o)20.9(l)]TJ
0 -1.1116 TD
[(sa)19.9(mp)19.6(le)19.6(s,)-361.4(bu)20.8(t)-369.8(a)-370.9(si)20.9(gn)15.1(i)20.9(ca)18.5(nt)-359.4(di)20.7(ff)15.1(er)19.7(en)19.7(ce)-361.6(wa)18.8(s)-369.5(f)0(o)20.9(u)0(n)15.1(d)-369.7(us)21(in)20.7(g)-369.7(H)0(T)18.8(S)0(-)]TJ
T*
[(FR)20(S.)-355.7(Of)-354.4(th)20.7(e)-365.1(c)0(r)19.7(y)0(o)15.1(g)0(e)19.7(n)0(i)20.7(c)0(a)18.5(l)0(l)20.6(y)-364(pr)15.1(es)19.9(er)19.7(ve)19.7(d)-364(s)0(a)19.9(m)0(p)19.6(l)0(e)13.8(s)0(,)-349.9(t)0(h)15(e)-365.1(co)19.7(nd)20.8(it)20.6(io)20.7(n)]TJ
0 -1.1174 TD
[(60)20.8(%)-347.7(D)0(M)13.3(E)0(M)18.7(,)-349(30)20.8(%)-347.7(F)0(B)14.2(S)0(,)-338.4(a)0(n)19.7(d)-352.4(10)20.8(%)-347.7(D)0(M)19.1(S)0(O)-342.7(r)0(e)19.7(s)0(u)21(l)0(t)14.8(e)0(d)-337.4(i)0(n)-342.1(l)0(o)20.7(w)0(e)18.8(r)]TJ
0 -1.1116 TD
[(ce)18.5(ll)-422.9(de)19.7(at)19.5(h)-433.1(t)0(h)20.7(a)0(n)-418.1(o)0(t)14.9(h)0(e)19.7(r)-427.3(cr)14(yo)20.8(ge)19.7(ni)20.7(c)-434.2(c)0(o)19.7(n)0(d)20.8(i)0(t)20.6(i)0(o)20.7(n)0(s)15.3(,)-429.6(bu)20.8(t)-433.2(w)0(a)18.8(s)-432.9(si)20.9(gn)20.8(if)20.7(-)]TJ
T*
[(ic)19.6(an)19.7(tl)20.6(y)-496.4(d)0(i)20.7(f)0(f)20.9(e)0(r)13.9(e)0(n)19.7(t)-490.8(fr)15.1(om)-481.5(th)20.7(e)-497.6(c)0(o)19.7(n)0(t)20.7(r)0(o)20.9(l)-496.6(sa)19.9(mp)19.6(le)19.6(s.)-488.2(Fo)21(ll)20.6(ow)20(in)20.7(g)-496.4(t)0(h)20.7(i)0(s)]TJ
T*
[(in)20.7(it)20.6(ia)19.5(l)-369.8(s)0(t)20.9(u)0(d)20.8(y)0(,)-361.6(t)0(h)20.7(e)-365.1(tw)14.1(o)-364(h)0(y)20.8(p)0(o)15.1(t)0(h)20.7(e)0(r)19.7(m)0(i)19.5(c)-370.9(an)19.7(d)-364(c)0(r)19.7(y)0(o)15.1(g)0(e)19.7(n)0(i)20.7(c)-370.9(co)19.7(nd)20.8(it)20.6(io)20.7(ns)]TJ
0 -1.1174 TD
[(as)19.9(so)21(ci)19.6(at)13.8(ed)-366.2(wi)19.8(th)-365.2(th)20.7(e)-376.6(l)0(o)15(w)0(e)18.8(s)0(t)-365(l)0(e)19.5(v)0(e)19.7(l)0(s)-370.8(o)0(f)-365(c)0(e)18.5(l)0(l)-365.3(d)0(e)19.7(a)0(t)13.8(h)-375.5(in)-365.2(ea)18.5(ch)-366.2(ca)18.5(se)]TJ
0 -1.1116 TD
[(we)18.8(re)-400.7(us)21(ed)-400.8(in)-399.7(st)20.9(ud)20.8(ie)19.5(s)-409.8(l)0(a)19.6(s)0(t)15.1(i)0(n)20.7(g)-404.3(7)-410(a)0(n)19.7(d)-410(14)-399.6(da)19.7(ys)-399.4(\(F)21(ig)20.7(.)-412.4(1)0(B)19.8(\))0(.)-396.2(C)0(e)18.6(l)0(l)]TJ
T*
[(de)19.7(at)19.5(h)-421.6(i)0(n)20.7(c)0(r)19.7(e)0(a)18.5(s)0(e)14.1(d)-415.8(wi)14.1(th)-411.3(st)20.9(or)20.9(ag)19.7(e)-422.7(t)0(i)20.6(m)0(e)-413.5(r)0(e)19.7(g)0(a)19.7(r)0(d)20.9(l)0(e)13.8(s)0(s)-410.8(o)0(f)-411.1(t)0(h)20.7(e)-422.7(pr)20.9(es)19.9(er)19.7(-)]TJ
0 -1.1174 TD
[(va)19.7(ti)20.6(on)-434.2(co)19.7(nd)20.8(it)20.6(io)20.7(n)-444.6(u)0(s)21(e)0(d)19.7(.)-446.9(At)-429.4(da)19.7(y)-444.6(7)0(,)-430.7(h)0(y)20.8(p)0(o)20.8(t)0(h)15(e)0(r)19.7(m)0(i)19.5(c)-440(pr)15.1(es)19.9(er)19.7(va)19.7(ti)20.6(on)]TJ
0 -1.1116 TD
[(us)21(in)20.7(g)-323.6(H)0(i)19.8(b)0(e)19.7(r)0(n)15.1(a)0(t)19.5(e)0(-)19.7(A)-324.5(on)20.8(ly)-313.4(ra)19.7(is)15.1(ed)-308.6(ce)12.8(ll)-313.5(de)19.7(at)19.5(h)-323.6(c)0(o)19.7(m)0(p)19.6(a)0(r)19.7(e)0(d)-314.4(w)0(i)19.8(t)0(h)-313.3(i)0(n)20.7(i)0(-)]TJ
T*
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0 -1.1116 TD
[(By)-314.3(da)19.7(y)-323.6(1)0(4)20.8(,)-326(ce)18.5(ll)-313.5(de)19.7(at)13.8(h)-323.6(i)0(n)-313.4(H)0(i)19.8(b)0(e)19.7(r)0(n)20.9(a)0(t)13.8(e)0(-)19.7(A)-324.5(sa)19.9(mp)19.6(le)19.6(s)-329.2(h)0(a)19.7(d)-323.6(in)20.7(cr)19.7(ea)18.5(se)19.9(d)]TJ
T*
[(to)-284.5(45)20.8(%)-295.9(a)0(n)19.7(d)-294.8(th)20.7(e)-296(o)0(t)20.7(h)0(e)19.7(r)-294.8(hy)20.8(po)15.1(th)20.7(er)19.7(mi)19.5(c)-296(c)0(o)19.7(n)0(d)20.8(i)0(t)20.6(i)0(o)15(n)-294.8(\(H)20(TS)19.8(-F)21(RS)20(\))-294.8(h)0(a)19.7(d)]TJ
0 -1.1174 TD
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/F10 1 Tf
8.9663 0 0 8.9663 310.8472 160.6676 Tm
[(Survival)-337(of)-330.4(Schwann)-334.1(cells)-332.5(in)-336.5(preserved)-335.2(EngNT)]TJ
/F4 1 Tf
9.843 0 0 9.843 320.8251 144.2267 Tm
[(Following)-569.7(initial)-574(experiments)-575.6(using)-569.9(simple,)-571.2(unaligned)]TJ
-1.0137 -1.1116 TD
[(collagen)-565.5(gels,)-561.9(the)-563.3(survival)-562.9(of)-560.9(Schwann)-563.7(cells)-560.8(in)-561(EngNT)]TJ
T*
[(constructs)-364.5(was)-368(measured)-364.6(after)-365(hypothermic)-367.2(preservation)-368.1(in)]TJ
0 -1.1174 TD
[(Hibernate-A)-253.7(or)-244.1(cryogenic)-249.7(preservation)-252.9(in)-250(60%)-246.3(DMEM,)-247.9(30%)]TJ
0 -1.1116 TD
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T*
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0 -1.1174 TD
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0 -1.1116 TD
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T*
[(on)20.8(ly)-250(in)20.7(cr)19.7(ea)18.5(se)14.1(d)-260.3(b)0(y)]TJ
/F11 1 Tf
7.2342 0 TD
(*)Tj
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[(10)20.8(%)-261.3(b)0(y)-249.9(d)0(a)19.7(y)-260.3(14)20.8(.)]TJ
/F7 1 Tf
8.9663 0 0 8.9663 59.7543 749.7069 Tm
[(PRESERVATION)-341.8(OF)-331.2(ENGINEERED)-338.3(NEURAL)-337.3(TISSUE)-28028.9(577)]TJ
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-1.0137 -1.1116 TD
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T*
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0 -1.1174 TD
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25.504 4.4523 TD
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8.9663 0 0 8.9663 313.0582 356.7117 Tm
[(Metabolic)-338.8(activity)-332.8(of)-336.7(Schwann)-334.1(cells)]TJ
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[(within)-337.9(EngNT)-337.7(postpreservation)]TJ
/F4 1 Tf
9.843 0 0 9.843 323.0361 329.2724 Tm
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T*
[(se)14.1(rv)20.9(ed)-343.2(sa)19.9(mp)19.6(le)19.5(s)-352.2(w)0(e)18.8(r)0(e)-337.4(r)0(e)19.7(t)0(u)15(r)0(n)20.9(e)0(d)-337.4(t)0(o)-342.1(s)0(t)20.9(a)0(n)19.7(d)0(a)13.9(r)0(d)-336.2(c)0(u)19.7(l)0(t)14.8(u)0(r)20.9(e)-347.8(co)19.7(nd)15.1(it)20.6(io)20.7(ns)]TJ
T*
[(an)13.9(d)-312.1(m)0(a)18.4(i)0(n)20.7(t)0(a)19.5(i)0(n)20.7(e)0(d)-302.9(f)0(o)15.1(r)-312.1(1)-312.1(a)0(n)19.7(d)-312.1(3)-312.1(d)0(a)19.7(y)0(s)-307.3(\()0(F)21(i)0(g)20.7(.)-314.4(3\))20.9(.)-314.5(P)0(r)21(e)0(s)19.9(e)0(r)19.7(v)0(e)13.9(d)-312.1(sa)19.9(mp)19.6(le)19.6(s)]TJ
0 -1.1174 TD
[(an)13.9(d)-323.6(t)0(h)20.7(o)0(s)21(e)-324.8(th)20.7(at)-320.3(ha)19.7(d)-323.6(b)0(e)19.7(e)0(n)-314.4(m)0(a)18.4(i)0(n)20.7(t)0(a)13.8(i)0(n)20.7(e)0(d)-314.4(i)0(n)-313.4(c)0(u)19.7(l)0(t)20.6(u)0(r)15.1(e)-324.8(\(D)20(ME)18.7(M)-324.5(1)0(0)20.8(%)]TJ
0 -1.1116 TD
[(FB)14.2(S\))-272.7(fo)20.9(r)-283.3(t)0(h)15(e)-284.5(du)20.8(ra)19.7(ti)20.6(on)-272.9(ha)19.7(d)-289.1(s)0(i)20.9(g)0(n)20.8(i)0()20.9(c)0(a)18.5(n)0(t)14.9(l)0(y)-273(d)0(e)19.7(c)0(r)19.7(e)0(a)18.5(s)0(e)19.9(d)-289.1(AT)18.8(P)-283.2(l)0(e)19.5(v)0(e)19.7(l)0(s)]TJ
T*
[(co)13.9(mp)19.6(ar)19.7(ed)-539(wi)19.8(th)-538(fr)20.9(es)19.9(h)-548.3(s)0(a)19.9(m)0(p)19.6(l)0(e)19.6(s)-548.1(an)19.7(al)13.8(yz)19.7(ed)-533.2(im)13.7(m)15(e)0(d)13.9(i)0(a)19.5(t)0(e)19.6(l)0(y)-538(a)0(f)19.7(t)0(e)19.6(r)]TJ
0 -1.1174 TD
[(co)13.9(ns)21(tr)20.7(uc)19.7(ti)20.6(on)15.1(.)-331.7(T)0(h)19.6(e)-330.6(sa)19.9(mp)19.6(le)19.6(s)-335(p)0(r)20.9(e)0(s)19.9(e)0(r)14(v)0(e)19.7(d)-329.4(in)-319.1(Hi)14.1(be)19.7(rn)20.9(at)19.6(e-)19.7(A)-336(s)0(h)21(o)0(w)20(e)0(d)]TJ
0 -1.1116 TD
[(a)-641.6(s)0(i)15.1(g)0(n)20.8(i)0()20.9(c)0(a)18.5(n)0(t)20.7(l)0(y)-635.9(h)0(i)20.7(g)0(h)20.8(e)0(r)-631.1(m)0(e)18.4(t)0(a)19.5(b)0(o)20.8(l)0(i)14.8(c)-641.6(ac)18.5(ti)20.6(vi)20.7(ty)-630.1(th)20.7(an)-631.2(eq)13.9(ui)20.7(va)19.7(le)19.6(nt)]TJ
T*
[(sa)14.1(mp)19.6(le)19.6(s)-237.1(m)0(a)18.4(i)0(n)20.7(t)0(a)19.6(i)0(n)20.7(e)0(d)-228(i)0(n)-227(c)0(u)19.7(l)0(t)14.8(u)0(r)20.9(e)-238.4(at)-228.1(th)20.7(e)-238.4(4)0(-)20.9(d)0(a)19.7(y)-237.2(ti)20.6(me)-229.2(po)20.8(in)20.7(t,)-229.3(bu)20.8(t)-237.4(t)0(h)20.7(i)0(s)]TJ
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/F10 1 Tf
8.9663 0 0 8.9663 313.0582 193.5496 Tm
[(Schwann)-340.4(cell)-333(and)-329.4(collagen)-338(alignment)-331(in)-336.5(EngNT)]TJ
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[(and)-335.7(guidance)-332.8(of)-336.7(neurite)-330.1(growth)-337.9(following)-337.4(preservation)]TJ
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9.843 0 0 9.843 323.0361 166.1669 Tm
[(Coculture)-462.8(experiments)-460.4(using)-454.7(primary)-459.4(rat)-459.6(DRG)-455.3(neurons)]TJ
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[(were)-595.1(used)-592.9(to)-589.8(investigate)-597.7(whether)-592.7(EngNT)-590.5(preserved)-594.9(for)]TJ
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[(3)-323.6(days)-322.2(in)-324.9(Hibernate-A)-322.8(\(hypothermic\))-329.1(or)-319(60%)-321.1(DMEM,)-328.5(30%)]TJ
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[(FB)14.2(S,)-321.2(an)19.7(d)-335.2(1)0(0)20.8(%)-336.2(DM)19.1(SO)-325.5(\(c)19.7(ry)20.9(og)20.8(en)19.7(ic)13.8(\))-329.4(w)0(o)14.2(u)0(l)20.7(d)-329.4(gu)15.1(id)20.7(e)-336.3(a)0(n)19.7(d)-329.4(su)15.3(pp)20.8(or)20.9(t)]TJ
T*
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T*
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T*
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ET
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1 i
96.831 732.245 m
517.323 732.245 l
517.323 532.29 l
96.831 532.29 l
W n
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420.87 0 0 199.956 96.831 532.29 cm
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BT
/F6 1 Tf
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[(FIG.)-256.1(1.)]TJ
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27.428 0 TD
[(do)21.1(tt)14.9(ed)-239.3(li)20.7(ne)]TJ
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[(:1)248.8(9)269.9(.)248.8(5)267.5(%)]TJ
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[(0.)24.4(05)15.3(,)]TJ
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ET
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238.044 0 0 169.795 62.759 163.389 cm
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[(FIG.)-284.9(2.)]TJ
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[(Comparison)-283.9(of)-284.2(Schwann)-280.6(cell)-283.1(death)-283.9(\(%\))-280.4(in)-284.3(EngNT)]TJ
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[(preserved)-449.9(using)-448.4(Hibernate-A)-454(\(hypothermic)-447.6(condition\))-449.2(and)]TJ
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[(60%)-378.3(DMEM,)-379.5(30%)-378.3(FBS,)-375.5(and)-378.4(10%)-378.3(DMSO)-380.6(\(cryogenic)-376.4(con-)]TJ
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T*
[(EngNT)-347.9(preserved)-346.2(using)-344.7(Hibernate-A)-344.6(was)-344.5(carried)-350.4(out)-342.9(to)-341.9(in-)]TJ
T*
[(clude)-404.8(days)-402.3(3)-398.3(and)-401.5(4.)-401.7(Day)-402.3(0)-404.1(represents)-404.8(unpreserved)-405.8(control)]TJ
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[(samples.)-468.6(ANOVA)-469.7(with)-471.6(Dunnetts)-466(post-tests)-471.8(revealed)-467.7(sig-)]TJ
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T*
[(day)-384.2(0)-381.1(\(***)]TJ
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[(6,)-378.7(data)-386.5(are)-385.4(mean)]TJ
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[(SEM.)-382.5(EngNT,)]TJ
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[(engineered)-337.2(neural)-336.7(tissue;)-336.5(FBS,)-335.2(fetal)-335.9(bovine)-329.8(serum.)]TJ
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49.0026 0 TD
[(DAY)-332.3(ET)-338.7(AL.)]TJ
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