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/F10 1 Tf
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[(mi)19.5(ni)20.7(ma)18.4(l)-329.5(e)0(s)19.9(s)0(e)19.9(n)0(t)20.7(i)0(a)13.8(l)-329.5(me)18.4(di)20.7(um)-320.2(an)19.7(d)-329.4(o)0(n)20.8(e)]TJ
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(m)Tj
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[(L)-226.9(p)0(e)19.7(r)-225.7(we)18.8(ll)]TJ
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()Tj
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[(C)-307.4(f)0(o)15.1(r)-312.1(15)-146.2(mi)19.5(n.)]TJ
/F11 1 Tf
8.9663 0 0 8.9663 310.8472 692.277 Tm
[(Proliferation)-338.4(assay)]TJ
/F4 1 Tf
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[(P)6.3(roli)6(f).4(era)5(tio)6.1(n)-477.7(o).4(f)-477.6(S).5(ch)6.1(wan)6.1(n)-477.7(cell)6(s)-477.5(on)-477.7(br)6.2(i).3(n)-477.7(a)-.8(n)6.1(d)-477.7(coll)6(age)5(n)]TJ
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[(\(BrdU\))-501.6(u)5.2(ptak)5.2(e)-502.8(a)4.1(ssay)-495.8(\()-.5(Cell)-496(Pro)5.2(liferat)5.1(ion)-495.8(E)-1.7(n)5.2(z)-1.7(yme-Li)5.1(nked)]TJ
T*
.0125 Tc
[(Immu)5.6(noso)5.6(rben)5.6(t)-484.1(Assay)-478.2([)-.1(ELISA])-478.1(Ki)5.5(t;)-484.1(Roch)5.6(e)-485.1(D)4.8(iagn)5.6(osti)5.5(cs,)]TJ
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[(Basel,)-415.6(Switzerland\))-419(ac)-5.5(cording)-419(t)1.3(o)-419(m).2(anufacturers)-418.8(instructions.)]TJ
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T*
-.0214 Tc
[(subs)-5(trate)-6.4(s)-316(\(7.5)]TJ
/F10 1 Tf
5.7194 0 TD
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()Tj
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(10)Tj
7.3823 0 0 6.5614 383.4141 614.4376 Tm
0 Tc
(3)Tj
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[(cells)-316.2(pe)-6.6(r)-316.4(w)-.5(ell)-316.6(o).3(f)-316.4(a)-317.6(96-w)-6.3(e)-.8(ll)-316.6(plat)-5.6(e\))-316.4(or)-316.4(poly-)]TJ
-8.0463 -1.1174 TD
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[(L-lysine)-518.3(c)-5.7(o)1.2(ated)-522.9(TCPS,)-519.5(respectively,)-519.5(100)]TJ
/F9 1 Tf
16.7204 0 TD
0 Tc
(m)Tj
/F4 1 Tf
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-.02 Tc
[(mol/L)-517.6(B).9(rdU)-517.3(w)1.1(as)]TJ
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[(added,)-237(and)-240.5(cells)-240.3(were)-241.6(incubate)-5.5(d)-234.7(f)-4.3(or)-240.4(24)-165.6(h)-240.5(a).3(t)-240.6(s)1.6(tandard)-240.5(cell)-234.8(c)-5.5(ulture)]TJ
T*
-.0218 Tc
[(condi)-5.8(tions)-443.2(\(3)-5.6(7)]TJ
/F10 1 Tf
5.6791 0 TD
0 Tc
()Tj
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[(C)-444.5(a)-1.1(nd)-443.5(5%)-438.8(C)-6.7(O)]TJ
7.3823 0 0 6.5614 429.392 575.5463 Tm
0 Tc
(2)Tj
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-.0216 Tc
[(\))-5.4(.)-439.7(Subs)-5.2(equen)-5.4(tly)-443.2(cultu)-5.4(re)-444.3(plates)]TJ
-12.3949 -1.1116 TD
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[(were)-455(xed)-453.9(with)-453.9(FixDenat)-454(solution)-453.9(and)-453.9(i)1(ncubated)-453.9(with)-453.9(anti-)]TJ
0 -1.1174 TD
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[(BrdU)-328.9(pero)-5.5(xidas)-5.3(e)-323.5(\(P)-5.4(OD\))-322.3(a)-6.7(n).2(tibo)-5.5(d).2(y)-322.3(s)-5.3(olutio)-5.5(n)-322.3(f).3(o)-5.5(r)-322.3(60)-172.5(min)-322.3(a)-.9(t)-328.2(r).3(oo)-5.5(m)]TJ
0 -1.1116 TD
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[(temper)-5.2(ature)-6.4(.)-439.5(Af)-5.2(ter)-442.9(w)-.3(as)-5(hing)-443(t)-5.3(h).5(e)-444.1(p).5(lat)-5.4(e)-444.1(with)-443(P)-5.1(B)-.5(S)-442.8(t).4(wic)-6.4(e)-.6(,)-439.5(t)-5.3(etra-)]TJ
T*
[(methyl)-5.5(benzid)-5.3(ine)-334.8(w)-.4(as)-339.2(added)-333.6(f)-5.3(or)-333.6(30)-166.6(mi)-5.4(n)-333.6(a)-.7(s)-333.4(a)-334.8(su)-5.3(bstra)-6.5(t).3(e.)-336(The)-334.8(r).5(e-)]TJ
0 -1.1174 TD
-.0205 Tc
[(action)-372.9(w)-5.2(as)-378.5(stopped)-378.7(with)-378.7(1)-165.6(m).2(ol/L)-374.1(H)]TJ
7.3823 0 0 6.5614 449.3479 520.7243 Tm
0 Tc
(2)Tj
9.843 0 0 9.843 452.8629 522.3683 Tm
-.0221 Tc
(SO)Tj
7.3823 0 0 6.5614 464.9384 520.7243 Tm
0 Tc
(4)Tj
9.843 0 0 9.843 468.4534 522.3683 Tm
-.0212 Tc
[(,)-376(a)-6.2(nd)-379.4(absorption)-379.4(w)-.1(as)]TJ
-16.012 -1.1116 TD
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[(measured)-367.6(at)-367.7(450)-367.6(with)-367.6(690)-166(nm)-368.8(as)-367.4(refere)-5.9(nce)-368.7(w).2(avelength)-367.6(o)1(n)-367.6(a)-.1(n)]TJ
T*
[(automa)-6(tic)-334.3(microplate)-334.3(r)-4.8(eader)-333.1(\(T)-6(ecan)-333.1(Sunrise\).)]TJ
/F11 1 Tf
8.9663 0 0 8.9663 310.8472 478.5447 Tm
0 Tc
[(Fabrication)-336.7(of)-330.4(stabilized)-333.6(collagen-brin)-334.9(blend)-337.1(gels)]TJ
/F4 1 Tf
9.843 0 0 9.843 320.8251 462.1038 Tm
[(Collagen-brin)-623.7(blend)-623.1(gels)-627.6(were)-623.9(prepared)-628.5(as)-625.2(follows:)]TJ
-1.0137 -1.1174 TD
(10)Tj
/F10 1 Tf
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()Tj
/F4 1 Tf
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[(MEM)-346.1(and)-350(Collagen)-346.5(I)-346.6(\(rat)-351.2(tail;)-347(First)-345.2(Link\))-346.6(were)-347.4(mixed)]TJ
-1.8949 -1.1116 TD
[(on)-267.1(ice)-270.7(in)-267.3(a)-273(ratio)-266.1(of)-272.9(1:8.)-266(Fibrinogen)-271.4(reconstituted)-277.3(in)-267.3(aprotinin)]TJ
T*
[(was)-281.6(added)-278.5(to)-278.8(the)-281.1(solution)-279.7(v/v)-274.1(ratios)-284.3(of)-278.6(10%,)-278.5(20%,)-278.5(30%,)-278.5(and)]TJ
T*
[(40%.)-468.6(Subsequently)-470.5(mixture)-465.4(was)-471.7(neutralized)-472.1(with)-466.3(7%)-469.8(v/v)]TJ
0 -1.1174 TD
[(sodium)-243.9(hydroxide.)-239.2(One)-247.2(unit)-240.8(thrombin)-240.7(in)-244.2(CaCl)]TJ
7.3823 0 0 6.5614 492.5479 405.6377 Tm
(2)Tj
9.843 0 0 9.843 498.614 407.2818 Tm
[(was)-247.1(added)-244(to)]TJ
-19.0761 -1.1116 TD
[(polymerize)-332.7(brinogen.)-331.2(Two)-333.7(hundred)-331.4(forty)-328.1(microliter)-337.6(of)-330.5(the)]TJ
T*
[(resulting)-374.1(collagen-brin)-376.2(solution)-371.9(was)-373.8(pipetted)-374.4(into)-367.5(96-well)]TJ
0 -1.1174 TD
[(plates)-279.7(and)-275.2(placed)-275.2(at)-280(37)]TJ
/F10 1 Tf
9.2443 0 TD
()Tj
/F4 1 Tf
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[(C.)-276.3(Gels)-277.1(were)-278.3(stabilized)-279.8(using)-276.1(RAFT)]TJ
-9.6417 -1.1116 TD
[(absorbers)-553.2(\(Lonza,)-554.9(Germany\))-548.8(for)-550.4(15)-169.2(min.)-549.5(Original)-552.6(height)]TJ
T*
[(before)-239.3(and)-234.9(after)-238.3(stabilization,)-240.7(as)-239.3(well)-237.1(as)-239.3(potential)-237.4(swelling)-241.4(of)]TJ
0 -1.1174 TD
[(the)-338.7(gels)-333.8(in)-336.4(300)]TJ
/F9 1 Tf
6.3011 0 TD
(m)Tj
/F4 1 Tf
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[(L)-336.4(medium,)-336.3(was)-339.2(evaluated)-343.1(with)-339.6(side)-333.8(eleva-)]TJ
-6.854 -1.1116 TD
[(tion)-333(view)-334.9(imaging)-332.9(\(KSV)-340(CAM)-334.4(200\))-332.7(on)-330.5(day)-338.5(0,)-332.8(1,)-332.8(3,)-332.8(and)-332.8(5.)]TJ
/F11 1 Tf
8.9663 0 0 8.9663 310.8472 308.6361 Tm
[(Mechanical)-332.9(testing)-333(of)-336.7(stabilized)-333.6(collagen)]TJ
0 -1.2203 TD
[(and)-329.4(collagen-brin)-341.3(blend)-330.8(gels)]TJ
/F4 1 Tf
9.843 0 0 9.843 320.8251 281.2535 Tm
[(M)15.3(e)15(ch)25.4(a)15(n)0(i)26.5(c)15(al)-291.5(t)16(e)0(s)25.6(t)0(i)26.3(n)16.2(g)-300.6(w)0(a)24.6(s)-300.4(pe)25.4(rf)26.7(o)16.2(r)0(m)25.4(e)15(d)-300.6(o)0(n)-290.2(a)-301.8(B)15.2(o)0(s)26.8(e)-301.8(E)15(l)0(e)25.3(c)0(t)25.3(r)16.2(oF)26.8(or)26.6(c)15(e)]TJ
-1.0137 -1.1116 TD
[(\()16.2(3)0(2)26.6(0)16.2(0)-461.9(S)16.3(e)15(r)0(i)26.5(e)0(s)-446.7(I)16.2(II)26.7(\))-456.1(u)0(s)26.8(i)0(n)26.5(g)-456.1(Wi)24.2(n)16.2(T)0(e)24.2(s)16.4(t)-462(7)-456.1(S)16.3(o)0(f)26.6(t)0(w)25.6(a)15(re)25.5(.)-458.4(C)15.2(ol)26.5(l)16.1(a)0(g)25.4(e)0(n)-446.9(a)15(n)16.2(d)]TJ
0 -1.1174 TD
[(c)15(o)0(l)26.5(l)16.1(ag)25.4(e)15(n)0(-)26.6()0(b)26.8(r)0(i)26.5(n)-168.1(b)16.2(l)0(e)25.3(n)16.2(d)-168.1(\()0(1)26.6(0)16.2(%,)-155.3(2)16.2(0)0(%)25.5(\))-168.1(t)16.1(e)0(s)25.6(t)-168.3(s)16.4(p)0(e)25.4(c)0(i)25.3(m)15(en)25.4(s)-167.9(w)15.3(er)25.5(e)-169.3(p)16.2(re)25.5(p)16.2(a)0(r)25.5(e)15(d)]TJ
0 -1.1116 TD
[(t)16.1(o)-185.4(b)16.2(e)-180.8(20)-140.4(m)15(m)-186.6(i)16(n)-179.6(le)25.3(n)16.2(g)0(t)26.5(h)0(,)-171.6(3)]TJ
/F10 1 Tf
9.3941 0 TD
()Tj
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[(0.)24.3(3)-150.8(m)15(m)-186.6(a)15(t)-179.8(t)0(h)26.5(e)-180.8(en)25.4(ds)-169(a)15(n)0(d)-169.2(2)]TJ
/F10 1 Tf
9.6187 0 TD
()Tj
/F4 1 Tf
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[(0.3)-171.7(m)-.2(m)-196(i)-15.1(n)]TJ
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0 Tc
[(t)16.1(h)0(e)-204.9(m)15(i)16(d)0(d)26.6(l)0(e)25.3(,)-216.5(a)15(n)16.2(d)-220(w)15.3(e)15(r)0(e)-204.9(p)16.2(la)25.3(ce)24.3(d)-214.2(b)0(e)25.4(t)16(we)24.6(e)15(n)-220(t)16.1(h)16.2(e)-221.1(i)16.1(n)16.2(s)0(t)26.7(r)0(u)26.6(m)15(en)25.4(t)-214.3(g)0(r)26.6(i)0(p)26.5(s)-214(wi)25.6(t)16(h)]TJ
0 -1.1174 TD
[(a)-422.7(g)0(a)25.4(u)0(g)26.6(e)-428.5(l)16.1(e)15(ng)26.6(th)-411.3(of)-411.1(12)-140.4(m)15(m)0(.)-414.7(T)15(es)25.6(ti)26.3(n)16.2(g)-427.3(w)15.3(a)0(s)-417.9(p)16.2(e)15(r)0(f)26.7(o)0(r)26.6(m)15(ed)-412.3(at)-412.4(23)]TJ
/F10 1 Tf
23.0043 0 TD
()Tj
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(C,)Tj
-23.3902 -1.1116 TD
0 Tc
[(a)15(p)0(p)26.6(l)16(yi)26.5(ng)-365.1(a)-376.6(l)16.1(oa)25.4(d)-375.5(o)0(f)-365(0)16.2(.)13.9(2)-156.6(g)16.2(/)16(s)-381(u)16.2(nt)26.5(i)16.1(l)-381.4(s)16.4(p)0(e)25.4(c)15(im)25.2(e)15(n)-375.5(fa)25.5(il)26.3(u)16.2(r)0(e)25.5(.)-377.8(D)15.3(a)0(t)25.3(a)-376.6(we)24.6(re)]TJ
T*
[(p)16.2(l)0(o)26.5(t)16.1(te)25.3(d)-484.9(a)15(s)-479(s)0(t)26.7(r)0(e)25.5(s)0(s)27(/)0(s)26.7(t)16.1(ra)25.5(in)-468.9(c)15(u)0(r)26.6(v)0(e)25.4(s)0(,)-470.9(a)15(n)16.2(d)-484.9(y)16.2(i)0(e)25.3(l)16.1(d)-484.9(s)16.4(tr)26.5(e)15(s)0(s)27(,)-487.2(a)15(s)-479(we)24.6(ll)-469(a)15(s)]TJ
0 -1.1174 TD
[(Y)15.3(o)0(u)26.6(n)16.2(g)26.6(s)-265.8(m)0(o)25.4(d)16.2(ul)26.5(us)-255.4(\()16.2(l)16(in)26.5(ea)24.3(r)-266(r)16.2(eg)25.4(io)26.5(n)16.2(\))0(,)-252.2(w)0(a)24.6(s)-265.9(ca)24.3(l)16.1(c)0(u)25.4(l)0(a)25.3(t)16(ed)25.4(.)]TJ
/F11 1 Tf
8.9663 0 0 8.9663 310.8472 171.6094 Tm
[(Viability)-332.8(of)-336.7(Schwann)-334.1(cells)-332.5(in)-336.5(stabilized)]TJ
0 -1.2203 TD
[(collagen-brin)-334.9(gels)]TJ
/F4 1 Tf
9.843 0 0 9.843 320.8251 144.2267 Tm
[(Su)15.2(rvi)13.9(val)-407.8(of)-411.1(SCL)11.9(4.)12.7(1/F)14(7)-415.8(cel)11.5(ls)-411.1(\(He)12(alt)12.6(h)-415.8(Pro)14.2(te)13.8(cti)12.6(on)-411.1(Age)11.9(ncy)12.8(,)]TJ
-1.0137 -1.1116 TD
[(UK\))-569.5(see)11.8(ded)-568.9(in)-572.5(sta)12.9(bil)13.7(ize)11.5(d)-577.1(col)12.7(la)13.8(gen)12.8(-b)14.2(rin)-567.8(ble)12.7(nd)-572.4(gel)12.7(s)-576.9(was)]TJ
T*
[(eva)11.7(lu)15(ate)11.5(d)-329.4(usi)14(ng)-330.5(MT)13(T)-330.6(ass)13.2(ay)-331.7(on)-324.7(day)-321.2(0,)-327.1(1,)-321.3(and)-321.2(3)-329.4(aft)12.7(er)-331.6(sta)12.9(bi)14.9(li-)]TJ
0 -1.1174 TD
[(zat)11.5(ion)19.6(.)-418.1(F)0(7)-411(SCL)11.9(4)-415.8(cel)11.5(ls)-411.1(on)-411.1(TCP)11.9(S)-415.6(act)11.5(ed)-412.3(as)-412.1(via)12.7(bil)13.7(ity)-406.7(con)12.8(tro)13.9(l.)]TJ
0 -1.1116 TD
[(Sta)12.9(bil)13.7(iz)13.8(ed)-475.7(gel)12.7(s)-479(wer)12(e)-480.3(inc)12.7(uba)12.8(ted)-471.1(wit)18.6(h)-479.2(cul)12.7(tur)13.9(e)-480.3(med)11.6(ium)-471.2(co)13.9(n-)]TJ
T*
[(tai)12.6(nin)13.8(g)-404.3(650)]TJ
/F9 1 Tf
4.8209 0 TD
(m)Tj
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T*
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T*
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T*
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T*
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