Hayashi, R;
Wainwright, SM;
Liddell, SJ;
Pinchin, SM;
Horswell, S;
Ish-Horowicz, D;
(2014)
A genetic screen based on in vivo RNA imaging reveals centrosome-independent mechanisms for localizing gurken transcripts in Drosophila.
G3
, 4
(4)
pp. 749-760.
10.1534/g3.114.010462.
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A genetic screen based on in vivo RNA imaging reveals centrosome-independent mechanisms for localizing gurken transcripts in Drosophila.pdf - Published Version Download (2MB) | Preview |
Abstract
We have screened chromosome arm 3L for ethyl methanesulfonate-induced mutations that disrupt localization of fluorescently labeled gurken (grk) messenger (m)RNA, whose transport along microtubules establishes both major body axes of the developing Drosophila oocyte. Rapid identification of causative mutations by single-nucleotide polymorphism recombinational mapping and whole-genomic sequencing allowed us to define nine complementation groups affecting grk mRNA localization and other aspects of oogenesis, including alleles of elg1, scaf6, quemao, nudE, Tsc2/gigas, rasp, and Chd5/Wrb, and several null alleles of the armitage Piwi-pathway gene. Analysis of a newly induced kinesin light chain allele shows that kinesin motor activity is required for both efficient grk mRNA localization and oocyte centrosome integrity. We also show that initiation of the dorsoanterior localization of grk mRNA precedes centrosome localization, suggesting that microtubule self-organization contributes to breaking axial symmetry to generate a unique dorsoventral axis.
| Type: | Article |
|---|---|
| Title: | A genetic screen based on in vivo RNA imaging reveals centrosome-independent mechanisms for localizing gurken transcripts in Drosophila |
| Location: | United States |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1534/g3.114.010462 |
| Publisher version: | http://dx.doi.org/10.1534/g3.114.010462 |
| Language: | English |
| Additional information: | Copyright © 2014 Hayashi et al. This is an open-access article distributed under the terms of the Creative Commons Attribution Unported License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
| Keywords: | RNA localization, microtubules, mutant mapping, oogenesis, whole-genome sequencing, Animals, Centrosome, Chromosome Mapping, Drosophila, Drosophila Proteins, Female, Genetic Testing, High-Throughput Nucleotide Sequencing, Kinesin, Male, Oocytes, Oogenesis, Polymorphism, Single Nucleotide, RNA, RNA, Messenger, RNA, Small Interfering, Sequence Analysis, DNA, Transforming Growth Factor alpha |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences > Cell and Developmental Biology |
| URI: | https://discovery.ucl.ac.uk/id/eprint/1474833 |
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