UCL Discovery
UCL home » Library Services » Electronic resources » UCL Discovery

Mechanisms of leading edge protrusion in interstitial migration.

Wilson, K; Lewalle, A; Fritzsche, M; Thorogate, R; Duke, T; Charras, G; (2013) Mechanisms of leading edge protrusion in interstitial migration. Nat Commun , 4 , Article 2896. 10.1038/ncomms3896. Green open access

[thumbnail of ncomms3896.pdf]
Preview
PDF
ncomms3896.pdf

Download (2MB)

Abstract

While the molecular and biophysical mechanisms underlying cell protrusion on two-dimensional substrates are well understood, our knowledge of the actin structures driving protrusion in three-dimensional environments is poor, despite relevance to inflammation, development and cancer. Here we report that, during chemotactic migration through microchannels with 5 μm × 5 μm cross-sections, HL60 neutrophil-like cells assemble an actin-rich slab filling the whole channel cross-section at their front. This leading edge comprises two distinct F-actin networks: an adherent network that polymerizes perpendicular to cell-wall interfaces and a 'free' network that grows from the free membrane at the cell front. Each network is polymerized by a distinct nucleator and, due to their geometrical arrangement, the networks interact mechanically. On the basis of our experimental data, we propose that, during interstitial migration, medial growth of the adherent network compresses the free network preventing its retrograde movement and enabling new polymerization to be converted into forward protrusion.

Type: Article
Title: Mechanisms of leading edge protrusion in interstitial migration.
Location: England
Open access status: An open access version is available from UCL Discovery
DOI: 10.1038/ncomms3896
Publisher version: http://dx.doi.org/10.1038/ncomms3896
Language: English
Additional information: © 2013 Macmillan Publishers Limited. All Rights Reserved. This article is licensed under a Creative Commons Attribution 3.0 Unported Licence. To view a copy of this licence visit http:// creativecommons.org/licenses/by/3.0/.
UCL classification: UCL
UCL > Provost and Vice Provost Offices > UCL BEAMS
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Maths and Physical Sciences
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Maths and Physical Sciences > London Centre for Nanotechnology
URI: https://discovery.ucl.ac.uk/id/eprint/1416432
Downloads since deposit
157Downloads
Download activity - last month
Download activity - last 12 months
Downloads by country - last 12 months

Archive Staff Only

View Item View Item