Crowley, C;
Butler, C;
Camilli, C;
Hynds, R;
Kolluri, KK;
Janes, SM;
De Coppi, P;
(2019)
Non-invasive longitudinal bioluminescence imaging of human mesoangioblasts in bioengineered esophagi.
Tissue Engineering Part C: Methods
, 25
(2)
pp. 103-113.
10.1089/ten.TEC.2018.0351.
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Abstract
Esophageal engineering aims to create replacement solutions by generating hollow organs using a combination of cells, scaffolds and regeneration-stimulating factors. Currently, the fate of cells on tissue-engineered grafts is generally determined retrospectively by histological analyses. Unfortunately, quality-controlled cell seeding protocols for application in human patients are not standard practice. As such, the field requires simple, fast and reliable techniques for non-invasive, highly specific cell tracking. Here, we show that bioluminescence imaging is a suitable method to track human mesoangioblast seeding of an esophageal tubular construct at every stage of the pre-clinical bioengineering pipeline. In particular, validation of bioluminescence imaging as longitudinal quantitative assessment of cell density, proliferation, seeding efficiency, bioreactor culture and cell survival upon implantation in vivo was performed against standard methods in 2D cultures and in 3D decellularized esophageal scaffolds. The technique is simple, non-invasive and provides information on mesangioblast distribution over entire scaffolds. Bioluminescence is an invaluable tool in the development of complex bioartificial organs and can assist in the development of standardized cell seeding protocols, with the ability to track cells from bioreactor through to implantation.
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