@prefix epid: .
@prefix dc: .
@prefix geo: .
@prefix bibo: .
@prefix owl: .
@prefix void: .
@prefix event: .
@prefix skos: .
@prefix xsd: .
@prefix cc: .
@prefix ep: .
@prefix foaf: .
@prefix rdf: .
@prefix rdfs: .
@prefix eprel: .
@prefix dct: .
<>
foaf:primaryTopic ;
rdfs:comment "The repository administrator has not yet configured an RDF license."^^xsd:string .
dc:format "text/html";
dc:title "HTML Summary of #1553215 \n\nFunctional characterisation of tumour-specific T cell responses in pancreatic cancer\n\n";
foaf:primaryTopic .
rdf:type bibo:Document,
ep:Document;
rdfs:label "Functional characterisation of tumour-specific T cell responses in pancreatic cancer (Text)"^^xsd:string .
eprel:isVersionOf ;
eprel:isVolatileVersionOf ;
eprel:islightboxThumbnailVersionOf ;
rdf:type ep:Document;
rdfs:label "Functional characterisation of tumour-specific T cell responses in pancreatic cancer (Other)"^^xsd:string .
eprel:isVersionOf ;
eprel:isVolatileVersionOf ;
eprel:ispreviewThumbnailVersionOf ;
rdf:type ep:Document;
rdfs:label "Functional characterisation of tumour-specific T cell responses in pancreatic cancer (Other)"^^xsd:string .
eprel:isVersionOf ;
eprel:isVolatileVersionOf ;
eprel:ismediumThumbnailVersionOf ;
rdf:type ep:Document;
rdfs:label "Functional characterisation of tumour-specific T cell responses in pancreatic cancer (Other)"^^xsd:string .
eprel:isVersionOf ;
eprel:isVolatileVersionOf ;
eprel:issmallThumbnailVersionOf ;
rdf:type ep:Document;
rdfs:label "Functional characterisation of tumour-specific T cell responses in pancreatic cancer (Other)"^^xsd:string .
eprel:isIndexCodesVersionOf ;
eprel:isVersionOf ;
eprel:isVolatileVersionOf ;
rdf:type ep:Document;
rdfs:label "Functional characterisation of tumour-specific T cell responses in pancreatic cancer (Other)"^^xsd:string .
rdf:_1 .
bibo:abstract "BACKGROUND AND AIMS: Pancreatic cancer (PC) has a poor prognosis and effective diagnostic tools and therapies are currently unavailable. This project has explored the role of T cell responses in PC patients as a potential therapy. METHODS: Serum mesothelin (MSLN) levels were tested in patients with pancreatic diseases. CD4+ and CD8+ T cell responses against full-length overlapping MSLN peptide pools were identified. CEA691, together with pre-chosen MSLN547 and WT1-126 were used to stimulate T cell lines from PC patients. Antigen-specific function and phenotype were characterised in the ex-vivo expanded T cell lines after repeated Ag exposure. Further experiments used inhibitory receptor blockade to augment the function of T cells isolated from cancer patients. RESULTS: Soluble MSLN was elevated in PC patients compared to normal controls, but could not distinguish the malignant from benign pancreatic disease. MSLN-specific CD4+ T cell responses were significantly increased in PC patients compared to controls, indicating an expansion of MSLN-specific CD4+ T cell in PC patients, and new epitopes were identified. It was possible to generate CEA691, WT126 and MSLN547-specific HLA-A*02 restricted T cell lines from PC patients. The ex vivo expanded CEA691-specific T cells demonstrated Ag-specific cytotoxicity and were able to recognize and kill HLA-A2+ CEA+ pancreatic cell lines in vitro. Blockade of PD-1 and TIM-3 further enhanced T cell function in vitro. CONCLUSION: We found that tumour associated antigen (TAA)-specific T cells were identifiable in the peripheral repertoire of patients with pancreatic cancer, and the function of these cells were preserved in some cases. Such antigen-specific function and cytotoxicity was more common in relatively early stages of the disease than the terminal stage, and PD-L1 blockade further improved the responses, suggesting that TAA-specific T cells together with checkpoint inhibition could be potential strategies for the treatment of pancreatic cancer."^^xsd:string;
bibo:authorList ;
bibo:presentedAt ;
bibo:status ;
dct:creator ;
dct:date "2017-04-28";
dct:isPartOf ;
dct:issuer ,
;
dct:title "Functional characterisation of tumour-specific T cell responses in pancreatic cancer"^^xsd:string;
ep:hasDocument ,
,
,
,
,
;
rdf:type bibo:Article,
bibo:Thesis,
ep:EPrint,
ep:ThesisEPrint;
rdfs:seeAlso .
dct:title "UCL"^^xsd:string;
rdf:type bibo:Conference .
dct:isPartOf ;
foaf:name "Immunity and Transplantation, UCL (University College London)"^^xsd:string;
rdf:type foaf:Organization .
dct:hasPart ;
foaf:name "UCL (University College London)"^^xsd:string;
rdf:type foaf:Organization .
foaf:familyName "Chen"^^xsd:string;
foaf:givenName "Y"^^xsd:string;
foaf:name "Y Chen"^^xsd:string;
rdf:type foaf:Person .