Howell, PGT; Boyde, A; (2003) Volumes from which calcium and phosphorus X-rays arise in electron probe emission microanalysis of bone: Monte Carlo simulation. CALCIFIED TISSUE INT , 72 (6) 745 - 749. 10.1007/s00223-002-2010-9.
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Monte Carlo simulations of trajectories for electrons with initial energy of 10 keV through 30 keV were used to map the 3D location of characteristic x-ray photon production for the elements C, P, and Ca until the electrons either escaped as backscattered electrons (BSE) or had insignificant energy. The x-ray production volumes for phosphorus slightly exceed those for calcium, but both greatly exceed the volume through which BSE travel prior to leaving the sample. The x-ray volumes are roughly hemispherical in shape, and the oblate spheroid from which BSE derive occupies only the upper third to half the volume of x-ray generation. Energy-dispersive x-ray emission microanalysis (EDX) may not be secure as a method for the quantitation of BSE images of bone in the scanning electron microscope (SEM). Ca:P elemental ratios from EDX analyses may also be imperfect.
|Title:||Volumes from which calcium and phosphorus X-rays arise in electron probe emission microanalysis of bone: Monte Carlo simulation|
|Keywords:||Ca : P ratio, EDX, BSE SEM, bone, excitation volume, emission volume, microanalysis, MEAN ATOMIC-NUMBER, BACKSCATTERING, COEFFICIENT, MICROSCOPY, RADIATION|
|UCL classification:||UCL > School of Life and Medical Sciences > Faculty of Life Sciences > Biosciences (Division of)|
UCL > School of Life and Medical Sciences > Faculty of Medical Sciences > Eastman Dental Institute
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