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Injection of a boar sperm factor causes calcium oscillations in oocytes of the marsupial opossum, Monodelphis domestica

Witton, CJ; Swann, K; Carroll, J; Moore, HDM; (1999) Injection of a boar sperm factor causes calcium oscillations in oocytes of the marsupial opossum, Monodelphis domestica. ZYGOTE , 7 (4) 271 - 277.

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Abstract

At fertilisation, the sperm triggers an abrupt and transient increase in intracellular calcium ([Ca2+](i)) in the oocyte cytoplasm. In eutherian mammals, oocytes exhibit multiple [Ca2+](i) transients which are necessary for egg activation. We investigated [Ca2+](i) in the marsupial opossum, Monodelphis domestica. Embryo development in this therian mammal is quite distinct from that in most Eutheria. Oestrus was induced in an adult female opossum by introduction of a male into her cage. Injection of a boar sperm extract induced repetitive increases in [Ca2+](i). Each oscillation travelled across and around the periphery of the egg in a wave-like manner. A control injection of KCl elicited no change in [Ca2+](i). This is the first observation of [Ca2+](i) oscillations in the oocyte of a marsupial. The repetitive nature of the [Ca2+](i) changes were more similar to those in oocytes of Eutheria than those in oocytes of non-mammalian vertebrates.

Type:Article
Title:Injection of a boar sperm factor causes calcium oscillations in oocytes of the marsupial opossum, Monodelphis domestica
Keywords:calcium, marsupial, Monodelphis domestica, oocyte, sperm factor, SHORT-TAILED OPOSSUM, SEA-URCHIN EGGS, CA2+ OSCILLATIONS, MOUSE OOCYTES, ASCIDIAN EGG, INOSITOL TRISPHOSPHATE, EMBRYONIC-DEVELOPMENT, MEIOTIC MATURATION, HAMSTER EGGS, BOVINE EGGS
UCL classification:UCL > School of Life and Medical Sciences > Faculty of Life Sciences > Biosciences (Division of) > Cell and Developmental Biology

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