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Characterization of CD3(+)CD4(-)CD8(-) (double negative) T cells in patients with systemic lupus erythematosus: activation markers

Anand, A; Dean, GS; Quereshi, K; Isenberg, DA; Lydyard, PM; (2002) Characterization of CD3(+)CD4(-)CD8(-) (double negative) T cells in patients with systemic lupus erythematosus: activation markers. LUPUS , 11 (8) 493 - 500. 10.1191/0961203302lu235oa.

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Abstract

Systemic lupus erythematosus (SLE) is characterized by B cell hyperactivity and the production of autoantibodies, some of which (antibodies to dsDNA) are thought to be pathogenic. T helper cells drive the production of autoantibodies and the aim of this study is to characterize phenotypically a subpopulation of T cells (the CD3(+)CD4(-)CD8(-), double negative (DN) T cells) previously identified as helping to enhance anti-DNA antibodies in patients with SLE. Data were obtained using FACS staining of DN T cells that had been purified from PBMCs by magnetic bead separation.The percentage of TCR alphabeta(+) DN T cells was found to be significantly higher in patients with SLE as compared with controls (P=0.02), although there was no significant increase in total percentage of DN T cells, which includes TCR gammadelta(+) cells. Activation markers HLA-DR and CD69, the costimulatory molecule CD28 and CTLA-4 were all expressed on the surface of a higher percentage of DN T cells in patients with SLE than in patients with rheumatoid arthritis (RA) or healthy controls (HC). More DN T cells from patients with SLE were of CD45RA phenotype than was found in controls, while CD45RO-expressing cells were reduced. In addition, DN T cells from patients with SLE expressed significantly higher levels of HLA-DR (P=0.006), CD28 (P=0.05), CTLA4 (P=0.03) and CD45RA (P=0.05) on the cell surface than those from the CD4/8 population. Correlation of expression of the markers measured with various parameters of disease activity and severity showed that high levels of HLA-DR expression correlated with high circulating serum C3 ( > 0.9 IU/ml), indicating that an activated phenotype is consistent with severe disease.

Type: Article
Title: Characterization of CD3(+)CD4(-)CD8(-) (double negative) T cells in patients with systemic lupus erythematosus: activation markers
DOI: 10.1191/0961203302lu235oa
Keywords: activation markers, double negative, SLE, T cells, ANTIGEN-PRESENTING MOLECULES, DISEASE-ACTIVITY, HELPER-CELLS, RECEPTOR, LYMPHOCYTES, EXPRESSION, CTLA-4, CD69, PROLIFERATION, ABNORMALITIES
UCL classification: UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Medicine
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Medicine > Inflammation
URI: http://discovery.ucl.ac.uk/id/eprint/99267
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