Characterization of DNA-Binding Proteins Using Multiplexed Competitor EMSA.
J MOL BIOL
714 - 717.
We describe a low-cost high-throughput technique to characterize nuclear protein DNA-binding interactions. This technique, known as Multiplexed Competitor Electrophoretic Mobility Shift Assay, uses a series of multiplexed oligonucleotide DNA consensus competitors, in combination with a standard electrophoretic mobility shift assay procedure, to efficiently characterize DNA-binding proteins. We show utility for the method to identify a previously unreported hepatocyte nuclear factor-3 site created in intron 8 of the lipoprotein lipase gene by a con-anon single-nucleotide polymorphism (rs327). (C) 2008 Elsevier Ltd. All rights reserved.
|Title:||Characterization of DNA-Binding Proteins Using Multiplexed Competitor EMSA|
|Keywords:||electrophoretic mobility shift assay, transcription factor, DNA competitor, HUMAN GENOME, IDENTIFICATION, SITES|
|UCL classification:||UCL > School of Life and Medical Sciences > Faculty of Population Health Sciences > Institute of Cardiovascular Science|
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