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Characterization of DNA-Binding Proteins Using Multiplexed Competitor EMSA

Smith, AJP; Humphries, SE; (2009) Characterization of DNA-Binding Proteins Using Multiplexed Competitor EMSA. J MOL BIOL , 385 (3) 714 - 717. 10.1016/j.jmb.2008.11.035.

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Abstract

We describe a low-cost high-throughput technique to characterize nuclear protein DNA-binding interactions. This technique, known as Multiplexed Competitor Electrophoretic Mobility Shift Assay, uses a series of multiplexed oligonucleotide DNA consensus competitors, in combination with a standard electrophoretic mobility shift assay procedure, to efficiently characterize DNA-binding proteins. We show utility for the method to identify a previously unreported hepatocyte nuclear factor-3 site created in intron 8 of the lipoprotein lipase gene by a con-anon single-nucleotide polymorphism (rs327). (C) 2008 Elsevier Ltd. All rights reserved.

Type:Article
Title:Characterization of DNA-Binding Proteins Using Multiplexed Competitor EMSA
DOI:10.1016/j.jmb.2008.11.035
Keywords:electrophoretic mobility shift assay, transcription factor, DNA competitor, HUMAN GENOME, IDENTIFICATION, SITES
UCL classification:UCL > School of Life and Medical Sciences > Faculty of Population Health Sciences > Institute of Cardiovascular Science

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