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SELECTIVE FLOCCULATION OF CELLULAR CONTAMINANTS FROM SOLUBLE-PROTEINS USING POLYETHYLENEIMINE - A STUDY OF SEVERAL ORGANISMS AND POLYMER MOLECULAR-WEIGHTS

SALT, DE; HAY, S; THOMAS, ORT; HOARE, M; DUNNILL, P; (1995) SELECTIVE FLOCCULATION OF CELLULAR CONTAMINANTS FROM SOLUBLE-PROTEINS USING POLYETHYLENEIMINE - A STUDY OF SEVERAL ORGANISMS AND POLYMER MOLECULAR-WEIGHTS. ENZYME MICROB TECH , 17 (2) 107 - 113.

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Abstract

Saccharomyces cerevisiae, Escherichia coli, and Pseudomonas putida homogenates were treated with polyethyleneimine (PEI) of various molecular weights and concentrations, and the removal of cellular contaminants noted. The optimum doses were determined and the effects of ionic strength observed. Although there was little variation between the abilities of the various PEIs to remove cellular contaminants, the possibility of floc redissolution at higher polymer concentrations occurred more with increased molecular weight of the polymer. Increasing the ionic strength decreased the occurrence of floc redissolution. Both E. coli and P. putida required a higher concentration of PEI than S. cerevisiae to achieve significant removal of cellular contaminants. Loss of soluble protein by absorption to flocs with E. coli was influenced strongly by ionic strength.

Type: Article
Title: SELECTIVE FLOCCULATION OF CELLULAR CONTAMINANTS FROM SOLUBLE-PROTEINS USING POLYETHYLENEIMINE - A STUDY OF SEVERAL ORGANISMS AND POLYMER MOLECULAR-WEIGHTS
Keywords: POLYETHYLENEIMINE, FLOCCULATION, LIPIDS, NUCLEIC ACID, COLLOIDAL DEBRIS, OPTIMIZATION, KINETICS, IONIC STRENGTH, NUCLEIC-ACIDS, PRECIPITATION, PARTICLES, SILICA, CELLS
UCL classification: UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > UCL BEAMS
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science > Dept of Biochemical Engineering
URI: http://discovery.ucl.ac.uk/id/eprint/98356
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