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Thermodynamic study of the binding of the tandem-SH2 domain of the Syk kinase to a dually phosphorylated ITAM peptide: Evidence for two conformers

Grucza, RA; Futterer, K; Chan, AC; Waksman, G; (1999) Thermodynamic study of the binding of the tandem-SH2 domain of the Syk kinase to a dually phosphorylated ITAM peptide: Evidence for two conformers. BIOCHEMISTRY-US , 38 (16) 5024 - 5033.

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Abstract

The cytosolic tyrosine kinase Syk is recruited to immune cell receptors via interactions of its tandem-SH2 domain with tyrosine-phosphorylated sequences called immune receptor tyrosine activation motifs (ITAMs). We have characterized the binding of the tandem-SH2 domain of Syk (Syk-tSH2) to a dually phosphorylated peptide derived from the ITAM of the T cell receptor CD3-epsilon subunit, The CD3-epsilon peptide binds with an affinity of LX-sl nM at 150 mM NaCl over the 4.5-30 degrees C temperature range that was studied. The enthalpy of binding, Delta H degrees(obs),, shows an unusual nonlinear dependence on temperature, suggesting the possibility of a temperature-dependent conformational equilibrium coupled to binding. This hypothesis was tested and confirmed by examining the temperature dependence of (I) the on-rate constant for binding and (2) the fluorescence of Syk-tSH2 and its CD3-epsilon peptide complex. The Delta H degrees(obs), K-obs, fluorescence, and kinetic data are all well described by a model incorporating the hypothesized conformational equilibrium. Circular dichroism spectra at various temperatures indicate that the conformational change is not due to a partial unfolding of the protein. We suggest that the conformational equilibrium enables Syk-tSH2 to exhibit flexibility in its binding modality, which may be necessitated by Syk's involvement in a wide variety of signal tranduction pathways.

Type: Article
Title: Thermodynamic study of the binding of the tandem-SH2 domain of the Syk kinase to a dually phosphorylated ITAM peptide: Evidence for two conformers
Keywords: PROTEIN-TYROSINE KINASE, CELL ANTIGEN RECEPTOR, SRC SH2 DOMAIN, AFFINITY IGE RECEPTOR, FC-GAMMA RECEPTOR, ACTIVATION MOTIF, PHOSPHOPEPTIDE BINDING, SIGNAL-TRANSDUCTION, HUMAN PLATELETS, HEAT-CAPACITY
UCL classification: UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences > Structural and Molecular Biology
URI: http://discovery.ucl.ac.uk/id/eprint/97112
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