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Stargazin attenuates intracellular polyamine block of calcium-permeable AMPA receptors

Soto, D; Coombs, ID; Kelly, L; Farrant, M; Cull-Candy, SG; (2007) Stargazin attenuates intracellular polyamine block of calcium-permeable AMPA receptors. NAT NEUROSCI , 10 (10) 1260 - 1267. 10.1038/nn1966. Gold open access

Abstract

Endogenous polyamines profoundly affect the activity of various ion channels, including that of calcium-permeable AMPA-type glutamate receptors (CP-AMPARs). Here we show that stargazin, a transmembrane AMPAR regulatory protein ( TARP) known to influence transport, gating and desensitization of AMPARs, greatly reduces block of CP-AMPARs by intracellular polyamines. By decreasing CP-AMPAR affinity for cytoplasmic polyamines, stargazin enhances the charge transfer following single glutamate applications and eliminates the frequency-dependent facilitation seen with repeated applications. In cerebellar stellate cells, which express both synaptic CP-AMPARs and stargazin, we found that the rectification and unitary conductance of channels underlying excitatory postsynaptic currents were matched by those of recombinant AMPARs only when the latter were associated with stargazin. Taken together, our observations establish modulatory actions of stargazin that are specific to CP-AMPARs, and suggest that during synaptic transmission the activity of such receptors, and thus calcium influx, is fundamentally changed by TARPs.

Type: Article
Title: Stargazin attenuates intracellular polyamine block of calcium-permeable AMPA receptors
Open access status: An open access publication
DOI: 10.1038/nn1966
Publisher version: http://ukpmc.ac.uk/abstract/MED/17873873
Keywords: FIBER-INTERNEURON SYNAPSES, LONG-TERM POTENTIATION, 2 DISTINCT MECHANISMS, GLUTAMATE RECEPTORS, KAINATE RECEPTORS, CA2+ PERMEABILITY, SUBUNIT, NEURONS, CHANNELS, TRAFFICKING
UCL classification: UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences > Neuro, Physiology and Pharmacology
URI: http://discovery.ucl.ac.uk/id/eprint/9508
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