Splenic dendritic cell subsets prime and boost CD8 T cells and are involved in the generation of effector CD8 T cells.
(pp. 15 - 19).
ACADEMIC PRESS INC ELSEVIER SCIENCE
The ability of the dendritic cell (DC) subsets, CD8alpha(+) and CD8alpha(-) DCs, to initiate a CD8 T cell response or to activate memory CD8 T cells and generate effector CD8 T cells has been controversial. In this study, we analyse the capacity of splenic DC subsets to induce CD8 T cell responses to a CD8 T cell epitope (pb9) of a malaria antigen. The administration of peptide-pulsed CD8alpha(-) or CD8alpha(+) DCs primes and boosts a. primed CD8 T cell response against the malaria epitope. In vitro, depletion of CD11c(+) DCs from mouse splenocytes, immunised with recombinant vaccinia virus Ankara (MVA) expressing pb9 epitope, significantly reduced the generation of pb9-specific IFNgamma producing effector CD8 T cells, indicating that splenic DCs are involved in the development of pb9-specific IFNgamma producing effector cells. Taken together, this result shows that both DC subsets have the ability to prime and boost CD8 T cell responses and are involved in the activation of memory CD8 T cells. (C) 2004 Elsevier Inc. All rights reserved.
|Title:||Splenic dendritic cell subsets prime and boost CD8 T cells and are involved in the generation of effector CD8 T cells|
|Keywords:||dendritic cell subsets, CD8 T cell, IFN gamma releasing cells, IN-VIVO, IMMUNE-RESPONSE, CD8-ALPHA(+), EXPRESSION, INDUCTION, TOLERANCE|
|UCL classification:||UCL > School of Life and Medical Sciences
UCL > School of Life and Medical Sciences > Faculty of Medical Sciences
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