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Role of Fc gamma RI (CD64) in erythrocyte elimination and its up-regulation in thalassaemia

Wiener, E; Allen, D; Porter, RJ; Wickramasinghe, SN; Porter, JB; Chinprasertsuk, S; Siripanyaphinyo, U; ... Wanachiwanawin, W; + view all (1999) Role of Fc gamma RI (CD64) in erythrocyte elimination and its up-regulation in thalassaemia. BRIT J HAEMATOL , 106 (4) 923 - 930.

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Abstract

To examine any role of the high affinity Fc gamma class I receptor (Fc gamma RI) (CD64) in erythrocyte elimination by mononuclear phagocytes (MP) in thalassaemia (thal), we investigated the in vitro interaction of beta-thalassaemic erythrocytes with monocytes (Mo) whose Fc gamma R expression had been modulated by cytokines.Treatment of Mo with interferon (IFN)-gamma or interleukin (IL)-10 which up-regulate Fc gamma RI, caused a dose-dependent increase in binding of beta-thalassaemic erythrocytes, whereas stimulation with IL-4 which down-regulates the receptor, reduced this interaction, in a dose-dependent manner, to that of normal erythrocytes. Binding of thalassaemic erythrocytes by IFN-gamma or IL-10-treated Mo was inhibited by Fc gamma RI-specific reagents. In addition, Mo expression of Fc gamma RI and HLA class II DR was determined by flow cytometry in Thai patients with HbH disease (alpha(1)/alpha(2) or alpha(1)/Hb Constant Spring) (n = 15) or beta degrees-thal/HbE (n = 16). In both groups of patients Fc gamma RI expression was increased as compared to normal controls (n = 14): mean fluorescence intensity (+/-SD) 124.79 +/- 38.77 in HbH disease and 121.86 +/- 18.23 in beta degrees-thal/HbE versus 91.94 +/- 17.36 in normal controls (P < 0.01 and P < 0.001, respectively). In contrast, HLA class II DR expression was similar in patients and controls.The results suggest that, in thalassaemia, up-regulated Fc gamma RI on mononuclear phagocytes plays a role in their interaction with erythrocytes and that this process can be modified by cytokines.

Type: Article
Title: Role of Fc gamma RI (CD64) in erythrocyte elimination and its up-regulation in thalassaemia
Keywords: beta-thalassaemia, erythrocytes, monocytes, Fc gamma RI, cytokines, HOMOZYGOUS BETA-THALASSEMIA, HUMAN MONOCLONAL IGG1, ANTI-D ANTIBODIES, RED-CELLS, HUMAN-MONOCYTES, MONONUCLEAR PHAGOCYTES, HEMICHROME BINDING, CYTOTOXIC ACTIVITY, ALPHA-THALASSEMIA, INTERFERON-GAMMA
UCL classification: UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Cancer Institute
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Cancer Institute > Research Department of Haematology
URI: http://discovery.ucl.ac.uk/id/eprint/91627
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