UCL logo

UCL Discovery

UCL home » Library Services » Electronic resources » UCL Discovery

The WD-repeats of Net2p interact with Dnm1p and Fis1p to regulate division of mitochondria

Cerveny, KL; Jensen, RE; (2003) The WD-repeats of Net2p interact with Dnm1p and Fis1p to regulate division of mitochondria. MOL BIOL CELL , 14 (10) 4126 - 4139. 10.1091/mbc.E03-02-0092.

Full text not available from this repository.

Abstract

The Net2, Fis1, and Dnm1 proteins are required for the division of mitochondria in the yeast Saccharomyces cerevisiae. Net2p has an amino-terminal region that contains predicted coiled-coil motifs and a carboxyl-terminal domain composed of WD-40 repeats. We found that the amino-terminal part of Net2p interacts with Fis1p, whereas the carboxyl-terminal region interacts with both Dnm1p and Fis1p. Overproduction of either domain of Net2p in yeast cells poisons mitochondrial fission, and the dominant-negative effect caused by the WD-repeats of Net2p is suppressed by increased levels of Dnm1p. Point mutations in the WD-region of Net2p or in the GTPase region of Dnm1p disrupt the normal Net2p-Dnm1p interaction, causing Net2p to lose its normal punctate distribution. Our results suggest that Dnm1p interacts with the WD-repeats of Net2p and in a GTP-dependent manner recruits Net2p to sites of mitochondrial division. Furthermore, our results indicate that Net2p is required for proper assembly of the mitochondrial fission components to regulate organelle division.

Type: Article
Title: The WD-repeats of Net2p interact with Dnm1p and Fis1p to regulate division of mitochondria
DOI: 10.1091/mbc.E03-02-0092
Keywords: DYNAMIN-RELATED GTPASE, RATE-LIMITING STEP, SACCHAROMYCES-CEREVISIAE, BETA-GAMMA, OUTER-MEMBRANE, MEDIATED ENDOCYTOSIS, VESICLE FORMATION, CRYSTAL-STRUCTURE, PROTEIN IMPORT, INNER MEMBRANE
UCL classification: UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences > Cell and Developmental Biology
URI: http://discovery.ucl.ac.uk/id/eprint/91456
Downloads since deposit
0Downloads
Download activity - last month
Download activity - last 12 months
Downloads by country - last 12 months

Archive Staff Only

View Item View Item